PINK1 Antibody (BC100-494)
PINK1 Antibody
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Distributor Data...
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anti-Serine/threonine protein kinase PINK1 mitochondrial antibody, anti-PTEN induced putative kinase protein 1 antibody, anti-PARK 6 antibody, anti-Serine/threonine kinase PINK1 mitochondrial antibody, anti-PTEN induced putative kinase 1 antibody, anti-PINK 1 antibody, anti-Phosphatase and tensin homolog antibody, anti-PARK6 antibody, anti-FLJ27236 antibody, anti-BRPK antibody, |
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PINK1 Antibody Summary: | | Gene: | PINK1 |
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| Purity: | IgG purified |
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| Host: | Rabbit |
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| | Specificity: | Pink1 |
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| Publications: | Antibody has been mentioned in at least 8 Publications. |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional PINK1 Products PINK1 Antibody Details: | Immunogen: | A synthetic peptide made to the human Pink1 protein sequence (between residues 175-250). |
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| Localization: | Mitochondrial |
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Species reactivity with these antibodies has not been determined.
Cat# BC 100-494 - the immunogen used for this antibody production has 100% homology with human protein. |
| Uses: | The theoretical molecular weight of human PNK1 is ~60 kDa. Optimal working dilutions should be determined by the investigator. -Immunoprecipitation: Not tested; Projected working range is 5-20 ug/mg. This antibody is also useful for immunocytochemistry/immunofluorescence. |
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| Dilutions: | Immunocytochemistry 1:50-1:200, Immunofluorescence 1:50-1:200, Peptide ELISA, Western Blot 1:500-1:1000 |
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| Unit Size: | 0.1 ml (also available: 0.025 ml (sample size)) |
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| Concentration: | 0.2 mg/ml |
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Notes: This antibody is considered to be Innovators Grade. Innovators Grade antibodies have been validated for limited uses and require additional characterization for most new species/applications. Packaging: | Storage: | Store at 4 °C. Do not freeze. |
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| Buffer: | TBS with 0.1% BSA |
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| Preservative: | Sodium Azide |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: General Background: Phosphatase and Tensin Homolog (PTEN) dephosphorylates lipids. Malfunctions in this contribute to many human cancers. PTEN induced putative kinase 1 (Pink1) was first discovered when researching the PTEN signaling pathway, thus Pink1 is also involved in many human cancers. It is found in the mitochondria and its homozygous C-terminus mutation has been implicated in the early development of Parkinson's disease.
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Images (1) Reviews (3) Related Products (35) Exploring PINK1 Working with PINK1
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Jump to: Publications, Images, Reviews, Ask a Question
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Working with PINK1
PINK1 Antibody Images (1) Immunocytochemistry: PINK1 Antibody [BC100-494] - Detection of PINK1 (Green) in HepG2 cells using BC100-494. Nuclei (Blue) are counterstained with Hoechst 33258. |
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Reviewed by:
Anonymous 7/5/2011
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Application:
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Western Blot
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Sample Tested:
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HeLa whole cell, mito extract
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Sample Loaded:
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20ug
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Species:
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Human
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Sample Pretreated:
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CCCP
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Results:
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Recognizes at least 3 bands = different cleavage products before and after stabilization.
Cross-reacting band in my HeLa cells just above the top PINK1 band.
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Blocking Buffer:
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5% milk in TBST
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Concentration:
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5%
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Blocking Time:
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1hr
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Blocking Temperature:
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RT
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Dilution Ratio:
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1:1000
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Incubation Time:
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1hr-ON
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Incubation Temperature:
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RT-4C Celsius
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Incubation Dilution Buffer:
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5%milk in TBST
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Description:
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anti-rabbit HRP
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Dilution Ratio:
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1:10000
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Method:
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ECL
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Exposure Time:
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brief
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Positive Control:
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no
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Negative Control:
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siRNA to PINK1
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Did you observe bands?:
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Yes
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Specific Bands:
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3 at correct sizes kDa
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Non-specific Bands:
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1 just above pink1 ~65-70 kDa
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Reviewed by:
Anonymous 11/10/2010
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Application:
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Western Blot
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Sample Tested:
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HeLa whole cell lysate
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Sample Loaded:
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20ug
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Species:
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Human
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Sample Pretreated:
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10 uM CCCP
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Dilution Ratio:
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1:1000
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Incubation Time:
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overnight
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Incubation Temperature:
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4 Celsius
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Description:
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anti-Rabbit IgG HRP-conjugated
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Dilution Ratio:
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1:2000
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Method:
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Amersham ECL
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Negative Control:
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HeLa K/O PINK1
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Did you observe bands?:
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Yes
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Specific Bands:
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expected kDa
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Reviewed by:
Anonymous 5/17/2010
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Application:
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Western Blot
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Sample Tested:
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Hela whole cell lysate
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Sample Loaded:
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60ug
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Species:
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Human
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Sample Pretreated:
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siRNA, proteasome inhibition, mitochondrial potential dissipator
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Lot:
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A32
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Results:
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this is one of the better PINK1 antibodies available, although there are a lot of background bands and the PINK1 signal is not strong.
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Pub Med ID:
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18397367
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PubMedPDF:
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pub_doc_8616_296_1274110477.pdf
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Blocking Buffer:
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Li-COR blocking buffer
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Blocking Time:
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2hrs
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Blocking Temperature:
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room temp
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Dilution Ratio:
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1:1000
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Incubation Time:
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overnight
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Incubation Temperature:
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4 Celsius
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Incubation Dilution Buffer:
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Li-COR blocking buffer
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Description:
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Li-COR IRdye 800CW
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Manufacturer Catalog #:
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926-32211
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Dilution Ratio:
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1:10000
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Method:
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Li-COR Odyssey Imager
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Positive Control:
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chemical treatment
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Negative Control:
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PINK1 siRNA
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Did you observe bands?:
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Yes
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Specific Bands:
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67, 55, 45 kDa
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Non-specific Bands:
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116, 70, 29, kDa
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Additional Comments:
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it is easier to observe the PINK1 bands if the non-specific bands are cut-off before antibody incubation. There is a strong band around 29kd.
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Publications (8)PINK1 Antibody Product Specific:- Meissner C, Lorenz H, Weihofen A, et al. The mitochondrial intramembrane protease PARL cleaves human Pink1 to regulate Pink1 trafficking. J. Neurochem. 2011 Jun.[PMID 21426348]
- Liu W, Vives-Bauza C, Acín-Peréz- R, et al. PINK1 defect causes mitochondrial dysfunction, proteasomal deficit and alpha-synuclein aggregation in cell culture models of Parkinson's disease. PLoS One. 2009;4(2):e4597.[PMID: 19242547]
more... - Cui J, Yao Q, Li S, et al. Glutamine deamidation and dysfunction of ubiquitin/NEDD8 induced by a bacterial effector family.Science. 2010 Sep 3;329(5996):1215-8. [PMID: 20688984]
- Lin W, Kang UJ. Characterization of PINK1 processing, stability, and subcellular localization.J Neurochem. 2008 Jul;106(1):464-74.[PMID: 1839736]
- M. Cui, X. Tang, W. V. Christian, et al. Perturbations in Mitochondrial Dynamics Induced by Human Mutant PINK1 Can Be Rescued by the Mitochondrial Division Inhibitor mdivi-1. J. Biol. Chem. 2010; 285 (15): 11740-11752. [PMID: 20164189]
- Zhou C, Huang Y, Shao Y, et al. The kinase domain of mitochondrial PINK1 faces the cytoplasm. Proc Natl Acad Sci U S A. 2008 Aug 19;105(33):12022-7. Epub 2008 Aug 7.
- Weihofen, A., B. Ostaszewski, et al. Pink1 Parkinson mutations, the Cdc37/Hsp90 chaperones and Parkin all influence the maturation or subcellular distribution of Pink1. Hum Mol Genet. 2008 Feb 15;17(4):602-16. Epub 2007 Nov 14.
- Exner, N., et al. Loss-of-function of human PINK1 results in mitochondrial pathology and can be rescued by parkin. J Neurosci. 2007 Nov 7;27(45):12413-8.
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Jump to: Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies
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