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PI 3 Kinase catalytic subunit gamma Antibody (NB100-1097)

PI 3 Kinase catalytic subunit gamma Antibody

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PI 3 Kinase catalytic subunit gamma Antibody Summary:
Species:Hu
Applications:IHC, PEP-ELISA
Clonality:Polyclonal
Gene:PIK3CG
Purity:Immunogen affinity purified
Host:Goat
 
Publications:Antibody has at least 1 Background Reference.
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
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PI 3 Kinase catalytic subunit gamma Antibody Details:
Isotype:IgG
Immunogen:Synthetic peptide VLGIKQGEKHSA, representing the C-Terminus of the human protein (residues 1091-1102), according to NP_002640.
Species Reactivity:

Human, Mouse, Dog

Applications:
Uses:This antibody is useful for Elisa and Western Blot. Western blot: Approx 110-120kDa band seen in Human Heart lysate (Predicted MW of 121kDa according to NP_002640).
Dilutions:Immunohistochemistry 1:200, Peptide ELISA 1:32000
Unit Size:0.1 mg
Concentration:0.5 mg/ml
Positive Controls:2 Positive Controls
Packaging:
Storage:Aliquot and store at -20 °C or -80 °C. Avoid freeze-thaw cycles.
Buffer:Tris saline containing [pH7.3] with 0.5% BSA
Preservative:0.02% Sodium Azide
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: PIK3CG
Entrez5294 (Human)
30955 (Mouse)
Swiss ProtNP_002640. (Human)
 
Background:

This gene encodes a protein that belongs to the pi3/pi4-kinase family of proteins. The gene product is an enzyme that phosphorylates phosphoinositides on the 3-hydroxyl group of the inositol ring. It is an important modulator of extracellular signals, including those elicited by E-cadherin-mediated cell-cell adhesion, which plays an important role in maintenance of the structural and functional integrity of epithelia. In addition to its role in promoting assembly of adherens junctions, the protein is thought to play a pivotal role in the regulation of cytotoxicity in NK cells. The gene is located in a commonly deleted segment of chromosome 7 previously identified in myeloid leukemias.
Images (1)
  • Western blot: PIK3CG Antibody [NB100-1097] staining (2ug/ml) of Human Heart lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour

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3 Primary Antibodies

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Exploring PI 3 Kinase catalytic subunit gamma

2 Related Research Areas

Working with PI 3 Kinase catalytic subunit gamma

1 Background References
Jump to: Publications, Images, Ask a Question

Working with PI 3 Kinase catalytic subunit gamma

PI 3 Kinase catalytic subunit gamma Antibody Images (1)
Western blot: PIK3CG Antibody [NB100-1097] staining (2ug/ml) of Human Heart lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour
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Publications (1)

PI 3 Kinase catalytic subunit gamma Antibody Background References:

  1. Stoyanov B et al. Cloning characterization of a G protein-activated human phosphoinositide-3 kinase. Science 269:690-3 (1995).
Protocols
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Jump to: Positive Controls, Antibody Pairs, Lysates, Primary Antibodies, RNAi, Secondary Antibodies

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PI 3 Kinase catalytic subunit gamma in Context

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