PI 3 Kinase catalytic subunit gamma Antibody Pair (DP0278)

PI 3 Kinase catalytic subunit gamma [p Ser1100] Antibody Pair

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PI 3 Kinase catalytic subunit gamma [p Ser1100] Antibody Pair Summary:
Species:Hu
Applications:PLA
Gene:PIK3CG
Specificity:Reacts with phosphoinositide-3-kinase, catalytic, gamma polypeptide.
 
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
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PI 3 Kinase catalytic subunit gamma [p Ser1100] Antibody Pair Details:
Modification:p Ser1100
Species Reactivity:

Human

Applications:
Uses:This product is useful for In Situ Proximity Ligation Assay (cell).
Dilutions:Proximity Ligation Assay
Unit Size: 1 set
Concentration:Concentration of individual antibodies may be found on the vial label.
Notes:
Antibody pair set content: 1. Phospho-PIK3CG S1100 rabbit MaxPab affinity purified polyclonal antibody (20 ul) In PBS (0.09% (w/v) sodium azide) 2. PIK3CG mouse monoclonal antibody (40 ug) In 1x PBS, pH 7.2 *Reagents are sufficient for at least 30-50 assays using recommended protocols.
Packaging:
Storage:Store at -80 °C. Avoid freeze-thaw cycles.
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: PIK3CG
Entrez5294 (Human)
 
Background:

This dual recognition antibody pair set comes with two antibodies, one against the PIK3CG protein, and the other against the specific S1100 phosphorylated site of PIK3CG for use in situ Proximity Ligation Assay.

Images (1)
  • BlobFinder) download from The Centre for Image Analysis at Uppsala University.">Staining of HeLa cell nucleus with DAPI (A), result of proximity ligation assay using dual recognition antibody pair set, rabbit MaxPab® affinity purified polyclonal antibody 1:1200 and mouse monoclonal antibody 1:50, with Duolink® 100 Detection Kit 613 (B), and composite image (C) of (A) and (B). Each red dot represents one single phosphorylated protein. The images were analyzed using an optimized freeware (<a href=BlobFinder) download from The Centre for Image Analysis at Uppsala University." id="image29991" title="Staining of HeLa cell nucleus with DAPI (A), result of proximity ligation assay using dual recognition antibody pair set, rabbit MaxPab® affinity purified polyclonal antibody 1:1200 and mouse monoclonal antibody 1:50, with Duolink® 100 Detection Kit 613 (B), and composite image (C) of (A) and (B). Each red dot represents one single phosphorylated protein. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University."/>

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PI 3 Kinase catalytic subunit gamma [p Ser1100] Antibody Pair Images (1)
BlobFinder) download from The Centre for Image Analysis at Uppsala University." />Staining of HeLa cell nucleus with DAPI (A), result of proximity ligation assay using dual recognition antibody pair set, rabbit MaxPab® affinity purified polyclonal antibody 1:1200 and mouse monoclonal antibody 1:50, with Duolink® 100 Detection Kit 613 (B), and composite image (C) of (A) and (B). Each red dot represents one single phosphorylated protein. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
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