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Protein Disulfide Isomerase/P4HB Antibody

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Product Details

Summary
Product Discontinued
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    • Catalog Number
      NBP1-49272
    • Availability
      Product Discontinued

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Protein Disulfide Isomerase/P4HB Antibody Summary

Immunogen
KLH-coupled synthetic peptide within residues 100-150 of human PDI (Swiss Prot: P07237).
Marker
Fibroblast Marker
Specificity
Rabbit Anti-PDI Polyclonal Antibody detects endogenous levels of human mouse and rat PDI. Positive Control: K562 HepG2 A431 Hela Ramos mouse brain and rat brain lysates
Clonality
Polyclonal
Host
Rabbit
Gene
P4HB
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • ELISA 0.05-0.2 ug/ml
  • Flow Cytometry 1-3 ug for 1 x 10^6 cells
  • Western Blot 0.5-1 ug/ml

Reactivity Notes

Human, Mouse, Rat

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
0.02% Sodium Azide
Concentration
Lyoph
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.

Alternate Names for Protein Disulfide Isomerase/P4HB Antibody

  • Cellular thyroid hormone-binding protein
  • collagen prolyl 4-hydroxylase beta
  • DSI
  • ERBA2L
  • GIT
  • glutathione-insulin transhydrogenase
  • P4HB
  • P4Hbeta
  • p55
  • PDI
  • PDIA1
  • PDIA1procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide (protein disulfide isomerase-associated 1)
  • PDIEC 5.3.4.1
  • PHDB
  • PO4DB
  • PO4HB
  • procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide
  • PROHB
  • PROHBprocollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide (protein disulfide isomerase; thyroid hormone binding protein p55)
  • Prolyl 4-hydroxylase subunit beta
  • prolyl 4-hydroxylase, beta polypeptide
  • protein disulfide isomerase family A, member 1
  • protein disulfide isomerase/oxidoreductase
  • protein disulfide isomerase-associated 1
  • protein disulfide-isomerase
  • protocollagen hydroxylase
  • Thbp
  • thyroid hormone-binding protein p55

Background

P4HB, the beta subunit of prolyl 4-hydroxylase, a highly abundant member of the PDI family. P4HB is a multifunctional enzyme, involved in hydroxylation of prolyl residues in preprocollagen, as well as continual rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol P4HB
Entrez
Uniprot