PDI Antibody (NB300-517)
PDI Antibody (RL90)
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Distributor Data...
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anti-Disulphide Isomerase antibody, anti-Thyroid Hormone Binding Protein p55 antibody, anti-Protein Disulphide Isomerase antibody, anti-Procollagen Proline 2 Oxoglutarate 4 Dioxygenase beta antibody, anti-Glutathione Insulin Transhydrogenase antibody, anti-GIT antibody, |
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PDI Antibody (RL90) Summary: | | Gene: | PDIA2 |
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| Purity: | Protein A purified |
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| Host: | Mouse |
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| | Specificity: | PDI (RL90) |
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| Publications: | Antibody has been mentioned in at least 7 Publications. |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional PDI Products PDI Antibody (RL90) Details: | Clone: | RL90 |
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| Isotype: | IgG2a |
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| Immunogen: | Purified rat PDI. |
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| Marker: | Endoplasmic Reticulum Marker |
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Cross-reacts with Human, Rat, Mouse, Hamster and Porcine. |
| Uses: | This antibody works in Flow cytometry / FACS analysis, Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation and Western Blot. |
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| Dilutions: | Flow Cytometry, Immunofluorescence 1:100, Immunohistochemistry-Frozen 1:100, Immunohistochemistry-Paraffin 1:100, Immunoprecipitation, Western Blot 1:1000 |
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| Unit Size: | 0.1 ml |
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| Concentration: | 1.0 mg/ml |
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Packaging: | Storage: | Store at -20 °C. Avoid freeze-thaw cycles. |
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| Buffer: | PBS with 1 mg/ml BSA and 0.05% sodium azide. |
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| Preservative: | 0.05% Sodium Azide |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: MA3-019 detects protein disulphide-isomerase (PDI) from human, rat, porcine and mouse tissues as well as hamster cells.
MA3-019 has been successfully used in Western blot, immunofluorescence, immunohistochemical, flow cytometry, and immunoprecipitation procedures. By Western blot, this antibody detects a protein at 59 kDa representing PDI from rat liver extract or a slightly higher protein at 61 kDa representing PDI from human liver extract. Immunohistochemical staining of PDI in rat intestine with MA3-019 yields a pattern consistent with cytoplasmic staining. In immunoprecipitation procedures MA3-019 has been shown to inhibit the activity of PDI in vitro. MA3-019 has also been found to inhibit disulfide bond reduction of the HIV protein, gp120, at the cell surface of CHO cells and human lymphoid cells.
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Images (4) Reviews (3) Related Products (9) Exploring PDI Working with PDI
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Jump to: Publications, Images, Reviews, Ask a Question
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Working with PDI
PDI Antibody (RL90) Images (4)-Immunofluorescence-NB300-517-img0001.jpg "Immunofluorescence: PDI Antibody (RL90) [NB300-517] - Immunofluorescence with anti-PDI Monoclonal Antibody [RL90].") Immunofluorescence: PDI Antibody (RL90) [NB300-517] - Immunofluorescence with anti-PDI Monoclonal Antibody [RL90]. | -Immunofluorescence-NB300-517-img0002.jpg "Immunofluorescence: PDI Antibody (RL90) [NB300-517] - Immunofluorescence with anti-PDI Monoclonal Antibody [RL90].") Immunofluorescence: PDI Antibody (RL90) [NB300-517] - Immunofluorescence with anti-PDI Monoclonal Antibody [RL90]. | -Immunofluorescence-NB300-517-img0003.jpg "Immunofluorescence: PDI Antibody (RL90) [NB300-517] - Immunofluorescence with anti-PDI Monoclonal Antibody [RL90].") Immunofluorescence: PDI Antibody (RL90) [NB300-517] - Immunofluorescence with anti-PDI Monoclonal Antibody [RL90]. | -Immunofluorescence-NB300-517-img0004.jpg "Immunofluorescence: PDI Antibody (RL90) [NB300-517] - Immunofluorescence with anti-PDI Monoclonal Antibody [RL90].") Immunofluorescence: PDI Antibody (RL90) [NB300-517] - Immunofluorescence with anti-PDI Monoclonal Antibody [RL90]. |
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Reviewed by:
Anonymous 6/20/2011
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Application:
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Western Blot
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Sample Tested:
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HL60 cells transduced with a lentiviral control or PDI shRNA vector.
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Sample Loaded:
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Lysates of 50,000 HL60 cells
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Species:
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Human
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Lot:
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LB138171 and MA151519
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Results:
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1. A band of protein disulfide isomerase is appeared at 65-kD.
I am very satisfied with the products supplied by NOVUS Biologicals, especially the high quality of many antibodies. In particular, I have been using monoclonal antibodies against protein disulfide isomerase since 2009. These antibodies work extremely well for detection of human protein disulfide isomerase and block its function well. The price of NOVUS Biologicals antibodies is very reasonable in comparison to that of other companies. I strongly recommend NOVUS Biologicals products to my colleagues.
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Blocking Buffer:
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5% BSA in TBS
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Blocking Time:
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1 hr
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Blocking Temperature:
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room temperature (RT)
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Dilution Ratio:
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1:1000
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Incubation Time:
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1 hr
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Incubation Temperature:
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RT
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Incubation Dilution Buffer:
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TBS containing 0.1% BSA and 0.1% Tween-20
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Description:
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HRP-conjugated anti-mouse IgG
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Manufacturer Catalog #:
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Jackson Immunoresearch, 715-035-150
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Dilution Ratio:
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1:10,000
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Method:
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Chemiluminescence
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Exposure Time:
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10 sec- 1min
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Did you observe bands?:
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Yes
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Specific Bands:
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PDI at 65 kDa
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Non-specific Bands:
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none kDa
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Reviewed by:
Jaehyung Cho 6/23/2010
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Application:
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Western Blot
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Sample Tested:
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cell lysate
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Species:
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Human
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Sample Pretreated:
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fMLP
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Results:
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This antibody was pure and worked fine.
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Reviewed by:
Anonymous 3/11/2010
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Application:
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Western Blot
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Sample Tested:
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plasma
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Sample Loaded:
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20ul
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Lot:
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KE132987
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Blocking Buffer:
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5% milk
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Concentration:
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5%
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Blocking Time:
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1 hr
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Blocking Temperature:
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Room temp
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Dilution Ratio:
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1/1000
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Incubation Time:
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overnight
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Incubation Temperature:
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room temp
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Incubation Dilution Buffer:
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1% milk
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Description:
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donkey anti mouse HRP
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Dilution Ratio:
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1/10000
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Method:
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ECL plus
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Exposure Time:
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2 sec
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Did you observe bands?:
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True
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Specific Bands:
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60 kDa
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Publications (7)
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Ask a Scientist
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Jump to: Primary Antibodies, Secondary Antibodies
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Related ProductsPrimary Antibodies (9)| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| PDI Antibody | NB100-1921 | Bv, Hu, Mk, Mu, Rt | ICC, IHC-Fr, IHC-P, IP, WB | (2) | (2) | | | | PDI Antibody (RL77) | NB600-1164 | Ha, Hu, Mu, Po, Rt, Xp | IHC-P, IP, WB | (4) | (3) | | | | PDI Antibody | PAB11963 | Am, Bv, Ca, GP, Ha, Hu, Mk, Mu, Po, Rt, Sh | ICC, IHC-P, IP, WB | | | | | | PDI Antibody | PAB12587 | Bv, Hu, Mu, Rt | ICC, IHC-Fr, IHC-P, IP, WB | | | | | | PDI Antibody | NBP1-49272 | Hu, Mu, Rt | ELISA, FACS, WB | (2) | | | | | ... and 4 more. See All » |
Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Mouse IgG Antibody [Biotin] | NB720-B | Mu | ELISA, IHC-P, WB | | | | | | Mouse IgG Antibody [HRP] | NB720-H | Mu | ELISA, ICC, IHC-P, WB | | | | | | Mouse IgG Antibody [Alkaline Phosphatase] | NB720-AP | Mu | ELISA, ICC, IHC-P, WB | | | | | | Mouse IgG Antibody [FITC] | NB720-F | Mu | ICC, IHC-P | | (2) | | | | Mouse IgG Antibody | NB120-7056 | Bv(-), Ch(-), Eq(-), Gp(-), Gt(-), Ha(-), Hu(-), Mu, Rb(-), Rt(-), Sh(-) | ELISA, IHC-P, IP, WB | | | | | | See All » |
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