Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
A synthetic peptide derived from aa
101-116 of human PDGF B form.
Localization:
Cytoplasmic
Epitope:
101-116aa
Species Reactivity:
Reacts with human and rat.
Applications:
Uses:
Western Blotting, Immunofluorence, Immunohistology (Formalin/paraffin) [Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.] The optimal dilution for a specific application should be determined by the investigator.
Dilutions:
immunohistochemistry, immunofluorescence, Western Blot
Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles.
Buffer:
Prepared in 10mM PBS, pH 7.6, with 0.2% BSA
Preservative:
0.09% Sodium Azide
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Platelet-Derived Growth Factor
(PDGF) is the major serum mitogen for cells of mesenchymal origin in humans. The biologically active protein is a dimer composed of two related polypeptides designated A and B. The PDGF protein has been implicated both directly as well as indirectly in several pathological states including neoplasia, arthritis, arteriosclerosis and bone marrow sclerosis. This antibody is specific for B form and reacts with
PDGF(AB) and PDGF(BB) protein.
Formalin-fixed, paraffin-embedded human angiosarcoma stained with PDGF antibody using peroxidase-conjugate and AEC. Note cytoplasmic staining of tumor cells