Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
Synthetic peptide: TEVFEISRRLIDRTNA, corresponding to amino acids 101-116 of Human PDGF beta.
Localization:
Cytoplasmic
Species Reactivity:
Cross-reacts with Human, Mouse and Rat. Not yet tested in other species.
Applications:
Uses:
WB: Use at an assay dependent dilution. Predicted molecular weight: 27 kDa. IHC-P: Use at a dilution of 1/100 for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Dilutions:
Immunohistochemistry-Frozen 1:50-1:100, Immunohistochemistry-Paraffin 1:50-1:100, Western Blot
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Platelet-Derived Growth Factor (PDGF) is the major serum mitogen for cells of mesenchymal origin in humans. The biologically active protein is a dimer composed of two related polypeptides designated A and B. The PDGF protein has been implicated both directly as well as indirectly in several pathological states including neoplasia, arthritis, arteriosclerosis and bone marrow sclerosis. This antibody is specific for B form and reacts with PDGF(AB) and
PDGF(BB) protein.