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PDGFA Antibody

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Product Details

Summary
Product Discontinued
View other related PDGFA Primary Antibodies

Order Details


    • Catalog Number
      NB200-633
    • Availability
      Product Discontinued

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PDGFA Antibody Summary

Immunogen
Synthetic peptide: TEVFEISRRLIDRTNA, corresponding to amino acids 101-116 of Human PDGF beta.
Localization
Cytoplasmic
Specificity
PDGF
Clonality
Polyclonal
Host
Rabbit
Gene
PDGFA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:100-1:2000
Application Notes
WB: Use at an assay dependent dilution. Predicted molecular weight: 27 kDa. IHC-P: Use at a dilution of 1/100 for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Cross-reacts with Human and Rat. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
10mM PBS (pH 7.4) and BSA
Preservative
Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PDGFA Antibody

  • PDGF-1
  • PDGF1PDGF A-chain
  • PDGF-A
  • Platelet-derived growth factor A chain
  • platelet-derived growth factor alpha chain
  • platelet-derived growth factor alpha isoform 2 preproprotein
  • platelet-derived growth factor alpha polypeptidePDGF subunit A
  • platelet-derived growth factor subunit A

Background

Platelet-Derived Growth Factor (PDGF) is the major serum mitogen for cells of mesenchymal origin in humans. The biologically active protein is a dimer composed of two related polypeptides designated A and B. The PDGF protein has been implicated both directly as well as indirectly in several pathological states including neoplasia, arthritis, arteriosclerosis and bone marrow sclerosis. This antibody is specific for B form and reacts with PDGF(AB) and PDGF(BB) protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional PDGFA Products

Research Areas for PDGFA Antibody (NB200-633)

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Blogs on PDGFA.

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Interleukin 6 is a complex pleiotropic cytokine having both anti and pro-inflammatory effects. Alterations in expression contribute to many human diseases, and the IL6 antibody is widely used in the research areas of innate and adaptive immunity, auto...  Read full blog post.

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Bioinformatics

Gene Symbol PDGFA
Entrez
Uniprot