PCNA Antibody (PC10) 0.1 ml Proliferation Marker

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Western Blot: PCNA Antibody (PC10) [NB500-106] - Analysis of human (HeLa lysate), murine (SV-T2 lysate), bovine (BAEC lysate), and porcine (PAE lysate) cell extracts.
Immunohistochemistry: PCNA Antibody (PC10) [NB500-106] - Analysis of PCNA in mouse cornea. Image courtesy of product review by Bo-Yie Chen.
Western Blot: PCNA Antibody (PC10) [NB500-106] - Hela cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-PCNA antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
Immunohistochemistry-Frozen: PCNA Antibody (PC10) [NB500-106] - IHC analysis of a paraformaldehyde fixed frozen section of brain from murine embryo using PCNA antibody clone PC10 (green), an IB4 antibody (red) and DAPI. Image provided by Dr Siegenthaler via product review.
Immunohistochemistry-Paraffin: PCNA Antibody (PC10) [NB500-106] - Analysis of paraffin-embedded human colon carcinoma, showing nuclear localization.
Simple Western: PCNA Antibody (PC10) [NB500-106] - Simple Western lane view shows a specific band for PCNA in 0.5 mg/ml of HeLa lysate.  This experiment was performed under reducing conditions using the 12-230 kDa separation system.
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Ordering Information: NB500-106

  • Catalog Number
    NB500-106
  • Displayed Format
    Unconjugated
  • Other Formats
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PCNA Antibody (PC10) Summary

Species Human, Mouse, Rat, Porcine, Chicken, Drosophila, Fish, Primate, Rabbit, Yeast, Zebrafish
Tested Applications WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, ChIP
Clonality
Monoclonal
Host
Mouse
Gene
PCNA
Purity
Protein A purified
Specificity
Specific for PCNA p36 protein expressed at high levels in proliferating cells.
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PCNA Antibody (PC10) Details

Clone
PC10
Isotype
IgG2a Kappa
Marker
Proliferation Marker
Immunogen
Protein A-rat PCNA fusion obtained from pC2T

Species Reactivity

Human, Yeast (S. pombe and S. cerevisiae), Drosophila melanogaster, Zebrafish, Rat, Mouse, Monkey, Chicken, Rabbit and Fish.
Reviewed Species Read 4 rated 4.8
using NB500-106 in the following species:

Publications Read Publications using NB500-106 in the following species:


Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Simple Western 1:200
  • ELISA
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:1000-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:500-1:1000
  • Immunoprecipitation 1:100
  • Chromatin Immunoprecipitation (ChIP)
Application Notes
This PCNA (PC10) antibody is useful for Immunohistochemistry on frozen and paraffin-embedded sections, Immunoprecipitation, Immunocytochemistry/Immunofluorescence (PMID: 22068968) Flow Cytometry and Western blot. It detects a band at 30 kDa by Western blot. For IHC on paraffin sections heat-mediated citrate buffer antigen retrieval is recommended.ELISA was reported in scientific literature.In Simple Western only 10-15 uL of the recommended dilution is used per data point. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in chromatin immunoprecipitation reported in scientific literature (PMID 26883631).
Molecular Weight
30 kDa
Positive Controls
Other Available
Formats
Alexa Fluor (R) 405 Labeled NB500-106AF405
Alexa Fluor (R) 488 Labeled NB500-106AF488
Alexa Fluor (R) 647 Labeled NB500-106AF647
Alexa Fluor (R) 700 Labeled NB500-106AF700
Allophycocyanin Labeled NB500-106APC
Biotin Labeled NB500-106B
DyLight 350 Labeled NB500-106UV
DyLight 405 Labeled NB500-106V
DyLight 405LS Labeled NB500-106V3
DyLight 488 Labeled NB500-106G
DyLight 550 Labeled NB500-106R
DyLight 650 Labeled NB500-106C
DyLight 680 Labeled NB500-106FR
DyLight 755 Labeled NB500-106IR
FITC Labeled NB500-106F
HRP Labeled NB500-106H
PE Labeled NB500-106PE
PerCP Labeled NB500-106PCP
Reviewed Applications
Read 4 Review rated 4.8
using
NB500-106 in the following applications:

Publications
Read Publications using
NB500-106 in the following applications:

Contact Information

Product PDFs

Frequently Purchased Together

Reviews

Average Rating: 4.8
Recent Reviews
Immunofluorescence
Reviewed On: 02/24/2014
Rated: 4

PCNA NB500-106
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Publications

PCNA Antibody (NB500-106) has been mentioned in at least 19 publications.
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Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-glycine, 150 mM NaCl
Unit Size
0.1 ml
Concentration
1 mg/ml
Preservative
0.05% Sodium Azide
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Gene Symbol PCNA
Entrez
Uniprot

Alternate Names for PCNA Antibody (PC10)

  • Cyclin
  • DNA polymerase delta auxiliary protein
  • MGC8367
  • proliferating cell nuclear antigen
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Related Products by Gene

Background

PCNA (Proliferating Cell Nuclear Antigen), plays a central role in coordinating the association of replication factors during DNA replication as well as during recognition and repair of DNA damage. PCNA forms a toroidal, ring-shaped structure of 90 kD by the symmetric association of three identical monomers. The ring encircles the DNA, acts as a platform upon which polymerases and other proteins dock to perform various DNA metabolic processes, and functions as a DNA polymerase-delta co-factor. The association of proteins with the replication fork through interactions with PCNA is regulated by several mechanisms. PCNA interacts with numerous proteins to facilitate DNA replication and repair, epigenetic processes and sister chromatid cohesion. PCNA has its highest expression during G1and S-phases, and its expression decreases in G2 and M-phases. This marker is also present in the early G0 phase because of its long half-live of 8-20 h. PCNA is a nuclear nonhistone protein that is necessary for DNA synthesis and is an accessory protein for DNA polymerase alpha, which is elevated during the G1/S phase of the cell cycle. Several studies have been performed to evaluate cell proliferation using PCNA and Ki-67 in different tumors of various origins; compared with PCNA, Ki-67 has been shown to be more sensitive and specific in the various tumors.

Reviews for PCNA Antibody (NB500-106) (4) 4.84

Read what people are saying who have used PCNA Antibody (NB500-106).

We have 3 reviews tested in 3 species: Human, Mouse, Other.
We have 4 reviews tested in 4: Western Blot, Immunohistochemistry-Paraffin, Immunofluorescence, Immunohistochemistry.
Average Rating: 4.8
(Based on 4 reviews)

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Sort 4 reviews for PCNA Antibody (NB500-106) by:
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(3)
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(1)
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Images Ratings Applications Species Reviewed By Date Details
  Excellent
WB Human Anonymous 08/23/2015
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Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell whole cell lysate
SpeciesHuman

Blocking

Blocking DetailsBlocking with 5% non-fat milk for 30 min

Primary Anitbody

Dilution Ratio1:2000, overnight

Secondary Antibody

Secondary Manufacturer Cat#GE Healthcare, NA931V
Secondary Concentration1:3000

Details

Detection NotesRegular Western blot Protocol
  Excellent
IHC-P Other simon hardy 03/28/2014
Show

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedChicken intestine
Sample Pretreatmentmicrowaved antigen retrieval ph6 in citrate
SpeciesOther
Other Specieschicken
CommentsVery strong signal at 1:1000. will try 1:2000 next time.

Primary Anitbody

Dilution Ratio1:1000

Details

Fixation Detailsformalin
Immunofluorescence PCNA NB500-106
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Very Good
IF Anonymous 02/24/2014
Show

Summary

ApplicationImmunofluorescence
LotNB500-106SS Lot A
Immunohistochemistry PCNA NB500-106
Click to Enlarge
Excellent
IHC Mouse Anonymous 02/06/2012
Show

Summary

ApplicationImmunohistochemistry
Sample TestedMouse cornea
SpeciesMouse

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:100, Primary Ab Incubation Time: 2 hours, Primary Ab Incubation Temp: room temperature

Details

Fixation DetailsSample Preparation: Paraffin Section Method

Publications for PCNA Antibody (NB500-106) (19)

Read what people are writing who have used PCNA Antibody (NB500-106).

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Fish, Porcine, Primate, Yeast.

We have publications tested in 8 applications: WB, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-P, IP.

Have you published using PCNA Antibody (NB500-106)? Submit your review and earn rewards points which can be used for merchandise & discounts.
Filter By Application
WB
(5)
ChIP
(1)
ELISA
(1)
Flow
(1)
ICC/IF
(2)
IHC
(4)
IHC-P
(9)
IP
(1)
All Applications
Filter By Species
Human
(6)
Mouse
(4)
Rat
(4)
Fish
(1)
Porcine
(1)
Primate
(1)
Yeast
(1)
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Publication Applications Species
Waseem NH Lane DP. Monoclonal antibody analysis of the proliferating cell nuclear antigen (PCNA). Structural conservation and the detection of a nucleolar form. J Cell Sci. 1990 May [PMID: 1695635] (WB, IP, ELISA, IHC-P, ICC/IF, Human, Primate) ELISA, ICC/IF, IHC-P, IP, WB Human, Primate
Williams SA, Wilson JB, Clark AP et al. Functional and physical interaction between the mismatch repair and FA-BRCA pathways. Hum Mol Genet. 2011 Aug 31. [PMID: 21865299]
Kuo W-L, Yu M-C, Lee J-F et al. Imatinib Mesylate Improves Liver Regeneration and Attenuates Liver Fibrogenesis in CCL(4)-Treated Mice. Journal of Gastrointestinal Surgery: Official Journal of the Society for Surgery of the Alimentary Tract. 2011 Nov 9. [PMID: 22068968] (IHC-P, ICC/IF, Mouse) ICC/IF, IHC-P Mouse
Woods AL et al. The assessment of proliferating cell nuclear antigen (PCNA) immunostaining in primary gastrointestinal lymphomas and its relationship to histological grade, S + G2 + M phase fraction (flow cytometric analysis) and prognosis. Histopathology;41(3A):165-71. 2002 Sep. [PMID: 12405950] (IHC-P, FLOW, Human) Flow, IHC-P Human
Ishikawa Y, Yamamoto N, Yasuda T et al. Morphogenesis of the medaka cerebellum, with special reference to the mesencephalic sheet, a structure homologous to the rostrolateral part of mammalian anterior medullary velum. Brain Behav Evol;75(2):88-103. 2010 [PMID: 20332601] (IHC-P, Fish) IHC-P Fish
Hasenstein JR, Kasmerchak K, Buehler D et al. Efficacy of tie2 receptor antagonism in angiosarcoma Neoplasia 2012 Feb [PMID: 22431921] (IHC, Human) IHC Human
Montaldi AP, Sakamoto-Hojo ET. Methoxyamine sensitizes the resistant glioblastoma T98G cell line to the alkylating agent temozolomide Clin Exp Med 2012 Jul 25 [PMID: 22828727] (WB, Human) WB Human
Chien YC, Huang GJ, Cheng HC et al. Hispolon Attenuates Balloon-Injured Neointimal Formation and Modulates Vascular Smooth Muscle Cell Migration via AKT and ERK Phosphorylation J Nat Prod 2012 Sep 28 [PMID: 22967007] (IHC, Mouse) IHC Mouse
Sheu MJ, Cheng HC, Chien YC et al. Molecular mechanism of green microalgae, Dunaliella salina, involved in attenuating balloon injury-induced neointimal formation. Br J Nutr 2010 Aug [PMID: 20370940] (IHC, WB, Rat, Human) IHC, WB Rat, Human
Sheu MJ, Lin HY, Yang YH et al. Demethoxycurcumin, a major active curcuminoid from Curcuma longa, suppresses balloon injury induced vascular smooth muscle cell migration and neointima formation: An in vitro and in vivo study Mol Nutr Food Res 2013 Mar 21 [PMID: 23520190] (IHC-P, Rat) IHC-P Rat
Errami Y, Brim H, Oumouna-Benachour K et al. ICAD deficiency in human colon cancer and predisposition to colon tumorigenesis: linkage to apoptosis resistance and genomic instability. PLoS One 2013 [PMID: 23451280] (IHC-P, Mouse) IHC-P Mouse
Zhang M, Wang F, Jiang L et al. Lactobacillus Salivarius REN Inhibits Rat Oral Cancer Induced by 4-Nitroquioline 1-Oxide. Cancer Prev Res (Phila) 2013 Jul [PMID: 23658366] (IHC-P, Rat) IHC-P Rat
Zhu J, Zhu C, Ge S et al. Lactobacillus salivarius Ren prevent the early colorectal carcinogenesis in 1, 2-dimethylhydrazine-induced rat model. J. Appl. Microbiol. 2014 Apr 22 [PMID: 24754742] (IHC-P, Rat) IHC-P Rat
Sawosz E, Jaworski S, Kutwin M et al. Toxicity of pristine graphene in experiments in a chicken embryo model Int J Nanomedicine 2014 Aug 25 [PMID: 25152621] (IHC) IHC
Houlihan SL, Feng Y. The scaffold protein Nde1 safeguards the brain genome during S phase of early neural progenitor differentiation. Elife. 2014 Sep 23 [PMID: 25245017] (IHC-P, Mouse) IHC-P Mouse
Kawamura K, Tachibana M, Sunanaga T. Cell proliferation dynamics of somatic and germline tissues during zooidal life span in the colonial tunicate Botryllus primigenus. Dev Dyn 2008 Jul [PMID: 18570248]
Engler A, Tange C, Frank-Bertoncelj M et al. Regulation and function of SIRT1 in rheumatoid arthritis synovial fibroblasts. J. Mol. Med. 2015 Aug 23 [PMID: 26298564] (WB, Human) WB Human
Ivanova IG, Maringele L. Polymerases epsilon and repair dysfunctional telomeres facilitated by salt. Nucleic Acids Res. 2016 Feb 15 [PMID: 26883631] (ChIP, Yeast) ChIP Yeast
Hu Y, Cong X, Chen L et al. Synergy of TLR3 and 7 ligands significantly enhances function of DCs to present inactivated PRRSV antigen through TRIF/MyD88-NF-kB signaling pathway. Sci Rep. 2016 Apr 06 [PMID: 27046485] (WB, Porcine) WB Porcine

Product General Protocols

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FAQs for PCNA (3)

  1. I am looking into preparing an exercise for an undergraduate lab that would immunostain drosophila testes. I think that PCNA Antibody (PC10) (cat # NB500-106) would be used as the primary antibody. What would you suggest to be used for the secondary? We have a fluorescent microscope and have used FITC in the past for some of our labs. Just trying to give the students a chance to visualize stained testes.
    • After looking at the applications of this antibody, I just found that we have not established this antibody for its Immunofluorescence application in our lab yet, however, as it has been tested successfully in IHC-P by our lab and by other researchers; there is a very strong chance that it should work in IHC-P/IF application also. If you would be interested in testing this novel application, please take a look at our Innovator's Reward Program. Regarding your specific query on secondary antibody, our FITC conjugated Mouse IgG antibody with catalog # NB720-F would be a perfect choice for primary PCNA Antibody (PC10) with catalog # NB500-106.
  2. Do you have data/information on the use of this antibody for Drosophila (mentioned on your website as possible target species)? I would like to know if any specific treatment is needed with regard to this type of sample and the use of the antibody in WB, IP in ICC.
    • This particular product is a widely licensed monoclonal clone. We obtained validation of its ability to detect the Drosophila protein from the following publication: PMID 22253867. You should be able to follow our general protocols for WB, IP, and ICC to detect this protein in their samples. Please see the following link for protocols.
  3. Can you please tell me the specific region of immunogen of this product?
    • This antibody was raised against whole rat PCNA protein fused to Protein A. As we have not yet epitope mapped this product, we do not know the exact immunogen recognized by this antibody.

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Sheep Polyclonal
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Western blot shows lysates of MCF‑7 human breast cancer cell line, non-transfected, transfected with plasmid expressing YFP-p27/Kip1 wild type (WT), or transfected with YFP-p27/Kip1 T198A mutant (T198A). PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human Phospho-p27/Kip1 (T198) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3994) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for p27/Kip1 was detected at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Species Human
Applications WB

9 Publications
NB100-56708
Western Blot: Caspase-3 Antibody (31A1067)  - (Pro and Active) [NB100-56708] - Analysis using Azide/BSA FREE version of NB100-56708. Analysis of Caspase-3 in A) RAW and B) NIH 3T3 using 32 ug/ml.Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067)  - (Pro and Active) [NB100-56708] - Analysis using the Azide Free version of NB100-56708. Staining of the human thymus after heat-induced antigen retrieval at 5 ug/ml.Western Blot: Caspase-3 Antibody (31A1067)  - (Pro and Active) [NB100-56708] - Western Blot Image of anti-Caspase 3 (31A1067).  Whole cell protein from Jurkat cells treated with and without 2 uM staurosporine as indicated was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST.  The membrane was probed with 5 ug/ml anti-Caspase 3 in 1% milk, and detected with an anti-mouse HRP secondary antibody using a Femto sensitivity chemiluminescence reagent.  Note the detection of both pro-caspase 3 at 35 kDa and the cleaved active caspase 3 at 15-17 kDa.

Mouse Monoclonal
Species Human, Mouse, Rat...MORE
Applications WB, Simple Western, ICC/IF...MORE

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93 Publications

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Blogs on PCNA

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Caspase 3 - a Reliable Marker for Index of Apoptosis Induction
Caspase-3 is one of the most important players in apoptosis signaling. It is synthesized as an inactive 32 kDa pro-enzyme and upon direct activation by Caspase-8, -9 or -10, it gets processed into its active forms, the p17-20 and p10-12 subunits. T...  Read full blog post.

PCNA (Proliferating cell nuclear antigen, polymerase delta auxiliary protein)
PCNA is a nuclear protein essential for DNA replication as well as DNA excision and mismatch repair pathways. It coordinates the recruitment and association of needed components during both of these processes, both of which are essential for cell cycl...  Read full blog post.

PCNA Antibodies: Marking Cell Proliferation & DNA Replication
Proliferating Cell Nuclear Antigen (PCNA), also known as the polymerase delta auxiliary protein, is a nuclear protein essential for DNA replication as well as DNA excision and mismatch repair pathways. It has a large role in cell cycle regulation and...  Read full blog post.

PCNA is a Universal Marker of Proliferating Cells
Proliferating cell nuclear antigen (PCNA) is an evolutionarily well-conserved protein found in all eukaryotic species as well as in Archaea. PCNA was first shown to be involved in DNA replication. However PCNA functions are associated with other vital...  Read full blog post.

Using PCNA as an Antibody Marker
PCNA antibodies are useful biomarkers in DNA repair studies. PCNA is one of several proteins essential for the completion of nucleotide excision repair, a multi-stage process involving 20 - 30 proteins, and an important factor in repairing damage and...  Read full blog post.