PCNA Antibody (PC10) 0.1 ml Proliferation Marker

PCNA NB500-106

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Ordering Information: NB500-106

Catalog NumberNB500-106
  • FormUnconjugated
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PCNA Antibody (PC10) Summary

 
Species Human, Mouse, Rat, Chicken, Drosophila, Fish, Primate, Yeast, Zebrafish
Tested Applications WB, ELISA, FLOW, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality Monoclonal
Host Mouse
Gene PCNA
Purity Protein A purified
Specificity Specific for PCNA p36 protein expressed at high levels in proliferating cells.
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PCNA Antibody (PC10) Details

 
Clone PC10
Isotype IgG2a Kappa
Marker Proliferation Marker
Immunogen Protein A-rat PCNA fusion obtained from pC2T

Species Reactivity

 
Human, Yeast (S. pombe and S. cerevisiae), Drosophila melanogaster, Zebrafish, Rat, Mouse, Monkey, Chicken and Fish.
Reviewed Species Read 2 rated 5  using NB500-106 in the following species:
 
Publications Read Publications using NB500-106 in the following species:
 

Applications/Dilutions

 
Dilutions
  • Western Blot 1:2000
  • ELISA
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:1000-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:500-1:1000
  • Immunoprecipitation 1:100
Application Notes

This PCNA (PC10) antibody is useful for Immunohistochemistry on frozen and paraffin-embedded sections, Immunoprecipitation, Immunocytochemistry/Immunofluorescence (PMID: 22068968) Flow Cytometry and Western blot. It detects a band at 30 kDa by Western blot. For IHC on paraffin sections heat-mediated citrate buffer antigen retrieval is recommended.ELISA was reported in scientific literature.

Molecular Weight

30 kDa

Positive Controls

2 Positive Controls

Other Available
Formats
7C Labeled NB500-106V2
Alexa Fluor (R) 405 Labeled NB500-106AF405
Alexa Fluor (R) 488 Labeled NB500-106AF488
Alexa Fluor (R) 647 Labeled NB500-106AF647
Alexa Fluor (R) 700 Labeled NB500-106AF700
Allophycocyanin Labeled NB500-106APC
Biotin Labeled NB500-106B
DyLight 350 Labeled NB500-106UV
DyLight 405 Labeled NB500-106V
DyLight 405LS Labeled NB500-106V3
DyLight 488 Labeled NB500-106G
DyLight 550 Labeled NB500-106R
DyLight 650 Labeled NB500-106C
DyLight 680 Labeled NB500-106FR
DyLight 755 Labeled NB500-106IR
FITC Labeled NB500-106F
HRP Labeled NB500-106H
PE Labeled NB500-106PE
PerCP Labeled NB500-106PCP
Learn about Custom Antibody Labeling
Reviewed Applications
Read 2 reviews rated 5  using
NB500-106 in the following applications:
Publications
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NB500-106 in the following applications:

Product PDFs

Reviews

Average Rating: 4.7 
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Publications

PCNA Antibody (NB500-106) has been mentioned in at least 13 publications.
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Protocols and FAQs

Controls & Support Products

Research Tools

View all PCNA Research Tools.

Packaging, Storage & Formulations

 
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer Tris-glycine, 150 mM NaCl
Unit Size 0.1 ml (also available in 0.025 ml (sample size))
Concentration 1 mg/ml
Preservative 0.05% Sodium Azide
Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

 
Gene Symbol PCNA
Entrez
Uniprot

Alternate Names for PCNA

 
  • Cyclin
  • DNA polymerase delta auxiliary protein
  • cyclin
  • MGC8367
  • proliferating cell nuclear antigen

Related Products by Gene

Background

 
PCNA (Proliferating Cell Nuclear Antigen), plays a central role in coordinating the association of replication factors during DNA replication as well as during recognition and repair of DNA damage. PCNA forms a toroidal, ring-shaped structure of 90 kD by the symmetric association of three identical monomers. The ring encircles the DNA, acts as a platform upon which polymerases and other proteins dock to perform various DNA metabolic processes, and functions as a DNA polymerase-delta co-factor. The association of proteins with the replication fork through interactions with PCNA is regulated by several mechanisms. PCNA interacts with numerous proteins to facilitate DNA replication and repair, epigenetic processes and sister chromatid cohesion. PCNA has its highest expression during G1and S-phases, and its expression decreases in G2 and M-phases. This marker is also present in the early G0 phase because of its long half-live of 8-20 h. PCNA is a nuclear nonhistone protein that is necessary for DNA synthesis and is an accessory protein for DNA polymerase alpha, which is elevated during the G1/S phase of the cell cycle. Several studies have been performed to evaluate cell proliferation using PCNA and Ki-67 in different tumors of various origins; compared with PCNA, Ki-67 has been shown to be more sensitive and specific in the various tumors.