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PCNA Antibody (PC10) 0.1ml Proliferation Marker

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Catalog Number  NB500-106
Sample Size Available
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Jump to Information on PCNA Antibody (NB500-106)

PCNA Antibody (PC10) Summary
 
Species Human, Mouse, Rat, Chicken, Drosophila, Primate, Yeast, Zebrafish
Tested Applications WB, FLOW, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality Monoclonal
Host Mouse
Gene PCNA
Purity Protein A purified
Specificity Specific for PCNA p36 protein expressed at high levels in proliferating cells.
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PCNA Antibody (PC10) Details
 
Clone PC10
Isotype IgG2a Kappa
Marker Proliferation Marker
Immunogen Protein A-rat PCNA fusion obtained from pC2T
Species Reactivity
 
Human, Yeast (S. pombe and S. cerevisiae), Drosophila melanogaster, Zebrafish, Rat, Mouse, Monkey and Chicken.
Reviewed Species Read 1 review rated 5  using NB500-106 in the following species:
 
Publications Read 5 publications using NB500-106 in the following species:
 
Applications/Dilutions
 
Dilutions
  • Western Blot 1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:1000-1:2000
  • Immunohistochemistry 1:10-1:2000
  • Immunohistochemistry-Frozen 1:10-1:2000
  • Immunohistochemistry-Paraffin 1:500-1:1000
  • Immunoprecipitation 1:100
Application Notes

This PCNA (PC10) antibody is useful for Immunohistochemistry on frozen and paraffin-embedded sections, Immunoprecipitation, Immunocytochemistry/Immunofluorescence (PMID: 22068968) Flow Cytometry and Western blot. It detects a band at 30 kDa by Western blot. For IHC on paraffin sections heat-mediated citrate buffer antigen retrieval is recommended.
Molecular Weight

30 kDa
Positive Controls

2 Positive Controls
Directly Conjugated
Formats
DyLight 488 Labeled NB500-106G
DyLight 650 Labeled NB500-106C
Biotin Labeled NB500-106B
DyLight 550 Labeled NB500-106R
HRP Labeled NB500-106H
FITC Labeled NB110-3158
Learn about Custom Antibody Labeling
Reviewed Applications
Read 1 review rated 5  using
NB500-106 in the following application:
Publications
Read 5 publications using
NB500-106 in the following applications:
  • WB2publications
  • IHC3publications
  • IHC-P1publication
Product PDFs
  Download PDF Datasheet
  Request CofA
  Request MSDS
Reviews
Average Rating: Excellent 
Recent Reviews

Test

  • {"id":2176,"rating":5,"ratetext":"5","title":"IHC Tested In Mouse","date":"02/06/2012"}

Read all 1 Review
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Publications
PCNA Antibody (NB500-106) has been mentioned in at least 10 publications.
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Controls & Support Products
View all related PCNA Controls and Support Products.
Research Tools
View all PCNA Research Tools.
Packaging, Storage & Formulations
 
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer Tris-glycine, 150 mM NaCl
Unit Size 0.1ml (also available in 0.025ml (sample size))
Concentration 1 mg/ml
Preservative 0.05% Sodium Azide
Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt.
Bioinformatics
 
Gene Symbol PCNA
Entrez
Uniprot
Alternate Names for PCNA
 
  • cyclin
  • Cyclin
  • DNA polymerase delta auxiliary protein
  • MGC8367
  • proliferating cell nuclear antigen
Related Products by Gene
 
CDKN1A, TP53, TCEAL1, NSG1, KI-67, CDK2, BCL2, CCND1, VEGFA, PAK3, EGFR, CDK4, PSMD9, TMED7, IFI27, DCTN6, SSSCA1, CDKN2A, CDKN1B, CASP3
Background
 
PCNA (Proliferating Cell Nuclear Antigen), plays a central role in coordinating the association of replication factors during DNA replication as well as during recognition and repair of DNA damage. PCNA forms a toroidal, ring-shaped structure of 90 kD by the symmetric association of three identical monomers. The ring encircles the DNA, acts as a platform upon which polymerases and other proteins dock to perform various DNA metabolic processes, and functions as a DNA polymerase-delta co-factor. The association of proteins with the replication fork through interactions with PCNA is regulated by several mechanisms. PCNA interacts with numerous proteins to facilitate DNA replication and repair, epigenetic processes and sister chromatid cohesion. PCNA has its highest expression during G1and S-phases, and its expression decreases in G2 and M-phases. This marker is also present in the early G0 phase because of its long half-live of 8-20 h. PCNA is a nuclear nonhistone protein that is necessary for DNA synthesis and is an accessory protein for DNA polymerase alpha, which is elevated during the G1/S phase of the cell cycle. Several studies have been performed to evaluate cell proliferation using PCNA and Ki-67 in different tumors of various origins; compared with PCNA, Ki-67 has been shown to be more sensitive and specific in the various tumors.