PARP Antibody (NSB698)
PARP Antibody
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Distributor Data...
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anti-ADP ribosyltransferase NAD+ antibody, anti-PPOL antibody, anti-Poly ADP ribose synthetase 1 antibody, anti-Poly ADP ribose polymerase 1 antibody, anti-Poly adenosine diphosphate ADP ribose polymerase antibody, anti-PARP1 antibody, anti-pADPRT1 antibody |
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PARP Antibody Summary: | | Gene: | PARP1 |
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| Purity: | Immunogen affinity purified |
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| Host: | Rabbit |
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| | Specificity: | This specifically recognizes the 85 kDa fragment of cleaved PARP and can be used as marker for detecting apoptotic cells. |
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| Publications: | Antibody has been mentioned in at least 2 Publications. |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional PARP Products PARP Antibody Details: | Isotype: | IgG |
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| Immunogen: | Synthetic peptide (Human) corresponding to N-terminus of cleavage site (214/215) of human PARP. |
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| Localization: | Nuclear |
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Cross-reacts with Cow and Human. Not yet tested in other species. |
| Uses: | Immunocytochemistry: Use at a dilution of 1/100. Western Blot: Use at a dilution of 1/1000. Predicted molecular weight: 85 kDa. Can be blocked with PARP Cleavage site antibody peptide. Optimal dilutions/concentrations should be determined by the end user. |
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| Dilutions: | Immunocytochemistry,
Western Blot, |
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| Unit Size: | 0.1 ml |
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| Concentration: | Please see the vial label for concentration. |
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| Positive Controls: | 1 Positive Controls |
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Packaging: | Storage: | Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles. |
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| Buffer: | PBS containing, 1mg/ml BSA and 50% Glycerol (IgG, protease free) |
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| Preservative: | 0.05% Sodium Azide |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Images (2) Reviews (0) Related Products (27) Exploring PARP Working with PARP
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Jump to: Publications, Images, Ask a Question
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Working with PARP
PARP Antibody Images (2) Western Blot: PARP Antibody [NSB698] - Western blotting Extracts from Jurkat cells untreated (1, 2) or stimulated with 0.5 µM staurosporine for 3 hours (3, 4) to induce apoptosis were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer overnight at 4 degrees C, and then incubated with the anti-PARP pan (1, 3) or PARP (214/215) cleavage site-specific antibody (CSSA) (2, 4) for two hours at room temperature in a 3% BSA-TBST buffer. After washing, the membrane was incubated with goat F(ab')2 anti-rabbit IgG HRP-conjugate and signals were detected using the Pierce SuperSignal(TM) method. The data show that the PARP (214/215) CSSA only recognizes the 85 kDa fragment of PARP in apoptotic cells (4) and does not react with full length PARP (2), demonstrating the specificity of the antibody. The anti-PARP pan confirms that non-apoptotic cells express full length PARP of 116 kDa (1), which is then cleaved when apoptosis is induced (3). |  Immunohistochemistry: PARP Antibody [NSB698] - Immunohistochemistry HeLa cells untreated (A) or induced into apoptosis with 0.5 mM staurosporine for 5 hours (B) and fixed in cold acetone for 5 minutes. Cells were incubated with the PARP (214/215) CSSA at 10 mg/mL. Cells were then incubated with biotinylated goat anti-rabbit IgG followed by ABC (Vector) and DAB. The data show that the PARP (214/215) CSSA specifically recognizes PARP in apoptotic cells. Taken together with Western blot results above, these data demonstrate the specificity of the antibody for cleaved PARP. |
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Publications (2)PARP Antibody Product Specific:- Leemans, J.C., et al. Depletion of alveolar macrophages exerts protective effects in pulmonary tuberculosis in mice. J. Immunol. 166(7):4604-4611, 2001.
- Tuturro, F., et al. Model of inhibition of the NPM-ALK kinase activity by herbimycin A. Clin. Cancer Res. 8(1):240-245, 2002.
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Jump to: Positive Controls, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies
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Related ProductsPositive Controls| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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Other Positive Controls- HeLa cells treated with staurosporine at 0.5 uM for 5 hours or bovine PARP cleaved by caspase 3.
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Peptides and ProteinsPrimary Antibodies (17)| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| PARP Antibody (C-2-10) | NB100-111 | Bv, Ch(-), Ha, Hu, Mk, Mu, Rt | ELISA, ICC, WB | (1) | (3) | | | | PARP Antibody (E102) | NB110-57323 | Hu, Mu(-), Rt | FACS, ICC, IHC-P, WB | (4) | (1) | | | | PARP Antibody (A6.4.12) | NB100-64828 | Dr, Hu, Mu, Rt, Xp | ELISA, IF, IHC-Fr, IHC-P, IP, WB | (1) | (3) | | | | PARP Antibody | NB100-61616 | Hu | IHC-P, WB | (3) | | | | | PARP Antibody (3G4) | H00000142-M01 | Hu | ELISA, IF, WB | (3) | (1) | | | | ... and 12 more. See All » |
RNAi (1)Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Rabbit IgG Antibody [HRP] | NB730-H | Rb | ELISA, ICC, IHC-P, WB | | (5) | | | | Rabbit IgG Antibody [FITC] | NB730-F | Rb | ICC, IHC-P | | (1) | | | | Rabbit IgG Antibody [Biotin] | NB730-B | Rb | ELISA, ICC, IHC-P, WB | | | | | | Rabbit IgG, H/L Chains Antibody [DyLight 488] | NBP1-72944 | Rb | FLISA, IF, WB | (3) | | | | | Rabbit IgG Antibody [Alkaline Phosphatase] | NB730-AP | Rb | ELISA, ICC, IHC-P, WB | | (1) | | | | See All » |
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