PARP Antibody (NB110-57323)

PARP Antibody (E102)

100% Guarantee This product is fully covered under the Novus guarantee+ Policy. Click here for details.

Distributor Data...

Loading Ajax
Loading...
Loading...Unit Size: 0.1 ml
 
Bulk Request
PARP Antibody (E102) Summary:
Species:Hu, Mu(-), Rt
Applications:FACS, ICC, IHC-P, WB
Clonality:Monoclonal
Gene:PARP1
Purity:Protein
Host:Rabbit
Specificity:The antibody should recognize both pro-form and p25 cleaved-form of PARP-1.
Publications:Antibody has at least 1 Background Reference.
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
View Additional PARP Products
PARP Antibody (E102) Details:
Clone:E102
Isotype:IgG
Immunogen:A synthetic peptide corresponding to N-terminal residues of human PARP-1 was used as immunogen.
Species Reactivity:

Reacts in Human, Rat. Does not react to Mouse.

Applications:
Uses:This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Flow Cytometry. In Western blot this antibody detects a band at approximately 116/25kDa.
Dilutions:Western Blot 1:1000-10000, Immunohistochemistry-Paraffin 1:25, Immunocytochemistry 1:100, Flow Cytometry 1:50
Unit Size: 0.1 ml
Concentration:Please see the vial label for concentration.
Packaging:
Storage:Store at -20 °C. Avoid freeze-thaw cycles.
Buffer:50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA.
Preservative:0.01% Sodium Azide
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: PARP1
Entrez142 (Human)
11545 (Mouse)
25591 (Rat)
Swiss ProtP09874 (Human)
 
Background:

Poly (ADP-ribose) polymerase (PARP) is zinc-dependent DNA binding protein that recognizes DNA strand breaks and is presumed to play a role in DNA repair (1). As a marker for apoptosis, PARP is cleaved in vitro by many caspases, and in vivo by Caspase-3 (2,3). Existing as a 116 kDa nuclear protein, PARP is cleaved between amino acids Asp214 and Gly215 to yield two fragments of 29 kDa (C-terminal catalytic domain) and 85 kDa (N-terminal DNA-binding domain) (2,4).

 
Jump to: Publications, Images, Protocols/Procedures, Ask a Question

Working with PARP

PARP Antibody (E102) Images (4)
Immunohistochemistry: PARP-1 Antibody (E102) [NB110-57323] - Immunohistochemical staining of paraffin-embedded human brain showing nuclear localization, using anti-PARP-1 (catalog #110-57323).Flow Cytometry: PARP-1 Antibody (E102) [NB110-57323] - Flow cytometric analysis of permeabilized Jurkat cells using anti-PARP-1 (red) (catalog #110-57323) or a rabbit IgG (negative) (green)
Western Blot: PARP-1 Antibody (E102) [NB110-57323] - Western blot analysis on (1) Jurkat and (2) Jurkat + Camptothecin cell lysates using anti-PARP-1 (catalog #110-57323), 1:1,000 dilution.PARP Antibody (E102)[NB110-57323]-IFA at an antibody dilution of 1:100 with antitmouse-FITC with counterstain
Reviews (0)  Have you used this product? Review this product to earn Novus Rewards.

Publications (1)

PARP Antibody (E102) Background References:

  1. Ikejima, M, et al. The zinc fingers of human poly(ADP-ribose) polymerase are differentially required for the recognition of DNA breaks nicks the consequent enzyme activation. Other structures recognize intact DNA. J. Biol. Chem. 265: 21907

Ask a Scientist

Can't find an answer to your question about PARP? Ask us by using live chat or by submitting your questions below:

 
Jump to: Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies

Related Products

Peptides and Proteins
NameCatalog#ReactivityApplicationsImagesPublicationsReviews
PARP ProteinNBP1-37088HuELISA, PAGE(1)

PARP Partial Recombinant ProteinH00000142-Q01HuELISA, WB(1)

PARP ProteinP3547HuPAGE

Primary Antibodies (17)
NameCatalog#ReactivityApplicationsImagesPublicationsReviews
PARP Antibody (C-2-10)NB100-111Bv, Ch(-), Ha, Hu, Mk, Mu, RtELISA, ICC, WB(1)(3)

PARP Antibody (A6.4.12)NB100-64828Dr, Hu, Mu, Rt, XpELISA, IF, IHC-Fr, IHC-P, IP, WB(1)(3)

PARP AntibodyNB100-61616HuIHC-P, WB(3)

PARP AntibodyNSB698Bv, HuICC, WB(2)(2)

PARP Antibody (3G4)H00000142-M01HuELISA, IF, WB(3)(1)

... and 12 more. See All »
RNAi (1)
NameCatalog#ReactivityApplicationsImagesPublicationsReviews
PARP RNAiH00000142-R01Hu(1)(4)

Secondary Antibodies
NameCatalog#ReactivityApplicationsImagesPublicationsReviews
Rabbit IgG Antibody [HRP]NB730-HRbELISA, ICC, IHC-P, WB(5)

Rabbit IgG Antibody [FITC]NB730-FRbICC, IHC-P(1)

Rabbit IgG Antibody [Biotin]NB730-BRbELISA, ICC, IHC-P, WB

Rabbit IgG, H/L Chains Antibody [DyLight 488]NBP1-72944RbFLISA, IF, WB(3)

Rabbit IgG Antibody [Alkaline Phosphatase]NB730-APRbELISA, ICC, IHC-P, WB(1)

See All »

Jump to: Novus Explorer, Research Areas

PARP in Context


Research Areas related to PARP (7):
Novus Explorer for PARP gene

Try our bioinformatic tool that shows the relationships between PARP1 and other genes, diseases, pathways and post-translational modifications.