PARP Antibody (NB110-57323)
PARP Antibody (E102)
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anti-ADP ribosyltransferase NAD+ antibody, anti-PPOL antibody, anti-Poly ADP ribose synthetase 1 antibody, anti-Poly ADP ribose polymerase family member 1 antibody, anti-Poly ADP ribose polymerase 1 antibody, anti-Poly adenosine diphosphate ADP ribose polymerase antibody, anti-PARP1 antibody, anti-PARP 1 antibody, anti-pADPRT1 antibody, anti-pADPRT 1 antibody, anti-NAD(+) ADP ribosyltransferase 1 antibody, anti-ADPRT1 antibody, anti-ADPRT antibody |
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PARP Antibody (E102) Summary: | | Gene: | PARP1 |
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| Purity: | Protein |
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| Host: | Rabbit |
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| | Specificity: | The antibody should recognize both pro-form and p25 cleaved-form of PARP-1. |
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| Publications: | Antibody has at least 1 Background Reference. |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional PARP Products PARP Antibody (E102) Details: | Clone: | E102 |
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| Isotype: | IgG |
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| Immunogen: | A synthetic peptide corresponding to N-terminal residues of human PARP-1 was used as immunogen. |
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Reacts in Human, Rat. Does not react to Mouse. |
| Uses: | This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Flow Cytometry. In Western blot this antibody detects a band at approximately 116/25kDa. |
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| Dilutions: | Western Blot 1:1000-10000, Immunohistochemistry-Paraffin 1:25, Immunocytochemistry 1:100, Flow Cytometry 1:50 |
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| Unit Size: | 0.1 ml |
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| Concentration: | Please see the vial label for concentration. |
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Packaging: | Storage: | Store at -20 °C. Avoid freeze-thaw cycles. |
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| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
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| Preservative: | 0.01% Sodium Azide |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: Poly (ADP-ribose) polymerase (PARP) is zinc-dependent DNA binding protein that recognizes DNA strand breaks and is presumed to play a role in DNA repair (1). As a marker for apoptosis, PARP is cleaved in vitro by many caspases, and in vivo by Caspase-3 (2,3). Existing as a 116 kDa nuclear protein, PARP is cleaved between amino acids Asp214 and Gly215 to yield two fragments of 29 kDa (C-terminal catalytic domain) and 85 kDa (N-terminal DNA-binding domain) (2,4).
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Images (4) Reviews (0) Related Products (27) Exploring PARP Working with PARP
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Jump to: Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies
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Related ProductsPeptides and ProteinsPrimary Antibodies (17)| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| PARP Antibody (C-2-10) | NB100-111 | Bv, Ch(-), Ha, Hu, Mk, Mu, Rt | ELISA, ICC, WB | (1) | (3) | | | | PARP Antibody (A6.4.12) | NB100-64828 | Dr, Hu, Mu, Rt, Xp | ELISA, IF, IHC-Fr, IHC-P, IP, WB | (1) | (3) | | | | PARP Antibody | NB100-61616 | Hu | IHC-P, WB | (3) | | | | | PARP Antibody | NSB698 | Bv, Hu | ICC, WB | (2) | (2) | | | | PARP Antibody (3G4) | H00000142-M01 | Hu | ELISA, IF, WB | (3) | (1) | | | | ... and 12 more. See All » |
RNAi (1)Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Rabbit IgG Antibody [HRP] | NB730-H | Rb | ELISA, ICC, IHC-P, WB | | (5) | | | | Rabbit IgG Antibody [FITC] | NB730-F | Rb | ICC, IHC-P | | (1) | | | | Rabbit IgG Antibody [Biotin] | NB730-B | Rb | ELISA, ICC, IHC-P, WB | | | | | | Rabbit IgG, H/L Chains Antibody [DyLight 488] | NBP1-72944 | Rb | FLISA, IF, WB | (3) | | | | | Rabbit IgG Antibody [Alkaline Phosphatase] | NB730-AP | Rb | ELISA, ICC, IHC-P, WB | | (1) | | | | See All » |
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![Immunohistochemistry: PARP-1 Antibody (E102) [NB110-57323] - Immunohistochemical staining of paraffin-embedded human brain showing nuclear localization, using anti-PARP-1 (catalog #110-57323).](http://images.novusbio.com/images/p_57323_2.jpg "Immunohistochemistry: PARP-1 Antibody (E102) [NB110-57323] - Immunohistochemical staining of paraffin-embedded human brain showing nuclear localization, using anti-PARP-1 (catalog #110-57323).")
![Flow Cytometry: PARP-1 Antibody (E102) [NB110-57323] - Flow cytometric analysis of permeabilized Jurkat cells using anti-PARP-1 (red) (catalog #110-57323) or a rabbit IgG (negative) (green)](http://images.novusbio.com/images/92688_4.jpg "Flow Cytometry: PARP-1 Antibody (E102) [NB110-57323] - Flow cytometric analysis of permeabilized Jurkat cells using anti-PARP-1 (red) (catalog #110-57323) or a rabbit IgG (negative) (green)")
![Western Blot: PARP-1 Antibody (E102) [NB110-57323] - Western blot analysis on (1) Jurkat and (2) Jurkat + Camptothecin cell lysates using anti-PARP-1 (catalog #110-57323), 1:1,000 dilution.](http://images.novusbio.com/images/p_57323_1.jpg "Western Blot: PARP-1 Antibody (E102) [NB110-57323] - Western blot analysis on (1) Jurkat and (2) Jurkat + Camptothecin cell lysates using anti-PARP-1 (catalog #110-57323), 1:1,000 dilution.")
![PARP Antibody (E102)[NB110-57323]-IFA at an antibody dilution of 1:100 with antitmouse-FITC with counterstain](http://images.novusbio.com/images/92688_5.jpg "PARP Antibody (E102)[NB110-57323]-IFA at an antibody dilution of 1:100 with antitmouse-FITC with counterstain")
