PARP Antibody (E102) (NB110-57323)

PARP Antibody (E102) (NB110-57323) ( 0.1 ml)

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	Catalog Number	NB110-57323
	Size(s)	0.1 ml	Price:
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anti-NAD(+) ADP-ribosyltransferase 1 antibody, anti-EC 2.4.2 antibody, anti-ADPRT 1 antibody, anti-poly(ADP-ribose) synthetase antibody, anti-PARP-1 antibody, anti-poly(ADP-ribose) polymerase antibody, anti-Poly[ADP-ribose] synthase 1 antibody, anti-poly(ADP-ribosyl)transferase antibody, anti-poly (ADP-ribose) polymerase family, member 1 antibody, anti-poly [ADP-ribose] polymerase 1 antibody, anti-ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase) antibody, anti-PPOLpADPRT-1 antibody, anti-ADPRTADP-ribosyltransferase NAD(+) antibody, anti-PARPADPRT1 antibody, anti-EC 2.4.2.30 antibody, anti-poly (ADP-ribose) polymerase 1 antibody

PARP Antibody (E102) Summary:

Species:	Hu, Mu, Rt	Gene:	PARP1
Applications:	WB, FACS, ICC, IHC-P	Purity:	Protein
Clonality:	Monoclonal	Host:	Rabbit
Specificity:	The antibody should recognize both pro-form and p25 cleaved-form of PARP-1.
Publications:	NB110-57323 has at least 1 Background Reference

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PARP Antibody (E102) Details:

Clone:	E102
Isotype:	IgG
Immunogen:	A synthetic peptide corresponding to N-terminal residues of human PARP-1 was used as immunogen.

Species Reactivity:

Human, Mouse, Rat. Cross reactivity determined by Western Blot only.

Applications:

Uses:	This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Flow Cytometry. In Western blot this antibody detects a band at approximately 116/25kDa.
Dilutions:	Western Blot 1:1000-10000, Immunohistochemistry-Paraffin 1:25, Immunocytochemistry 1:100, Flow Cytometry 1:50
Unit Size:	0.1 ml
Concentration:	Please see the vial label for concentration.

Packaging:

Storage:	Store at -20°C. Avoid freeze-thaw cycles.
Buffer:	50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA.
Preservative:	0.01% Sodium Azide
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Gene Symbol: PARP1

Entrez:	142 (Human)
	11545 (Mouse)
	25591 (Rat)
Uniprot:	P09874 (Human)

Related Genes:

Background:

Poly (ADP-ribose) polymerase (PARP) is zinc-dependent DNA binding protein that recognizes DNA strand breaks and is presumed to play a role in DNA repair (1). As a marker for apoptosis, PARP is cleaved in vitro by many caspases, and in vivo by Caspase-3 (2,3). Existing as a 116 kDa nuclear protein, PARP is cleaved between amino acids Asp214 and Gly215 to yield two fragments of 29 kDa (C-terminal catalytic domain) and 85 kDa (N-terminal DNA-binding domain) (2,4).

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PARP Antibody (E102) Images (4)

	Western Blot: PARP-1 Antibody (E102) [NB110-57323] - Western blot analysis on (1) Jurkat and (2) Jurkat + Camptothecin cell lysates using anti-PARP-1 (catalog #110-57323), 1:1,000 dilution.

	Immunohistochemistry: PARP-1 Antibody (E102) [NB110-57323] - Immunohistochemical staining of paraffin-embedded human brain showing nuclear localization, using anti-PARP-1 (catalog #110-57323).

	Flow Cytometry: PARP-1 Antibody (E102) [NB110-57323] - Flow cytometric analysis of permeabilized Jurkat cells using anti-PARP-1 (red) (catalog #110-57323) or a rabbit IgG (negative) (green)

	PARP Antibody (E102)[NB110-57323]-IFA at an antibody dilution of 1:100 with antitmouse-FITC with counterstain

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Publications (1)

PARP Antibody (E102) Background References:

Ikejima, M, et al. The zinc fingers of human poly(ADP-ribose) polymerase are differentially required for the recognition of DNA breaks nicks the consequent enzyme activation. Other structures recognize intact DNA. J. Biol. Chem. 265: 21907