PARP Antibody (NB100-78366)
PARP Antibody (5A5) [Biotin]
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Distributor Data...
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anti-Poly (ADP-ribose) polymerase antibody, |
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PARP Antibody (5A5) [Biotin] Summary: | | Gene: | PARP1 |
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| Purity: | Immunogen affinity purified |
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| Host: | Mouse |
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| | | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional PARP Products PARP Antibody (5A5) [Biotin] Details: | Conjugate: | Biotin |
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| Clone: | 5A5 |
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| Isotype: | IgG1 Kappa |
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| Immunogen: | Recombinant (partial), N-terminal 2/3 sequence of PARP |
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| Uses: | Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 mug antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application. |
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| Dilutions: | immunofluorescence
Western Blot |
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| Unit Size: | 0.1 mg |
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| Concentration: | 0.5 mg/ml |
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| Positive Controls: | 1 Positive Controls |
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| Conjugated Antibodies: | 1 Conjugated Antibodies |
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Packaging: | Storage: | Store at 4 °C. Do not freeze. |
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| Buffer: | PBS [pH 7.2] |
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| Preservative: | 0.09% Sodium Azide |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: PARP (Poly (ADP-ribose) polymerase) is a 113 kD nuclear protein that can exist as a homo- or hetero-dimer. This protein acts as a molecular "nick sensor" and functions in base excision repair, poly(ADP-ribosyl)ation of acceptor proteins involved in chromatin architecture and DNA metabolism and participates in protein modification to enhance or repress transcription. PARP is ribosylated by PARP2 and is a target for caspase cleavage during apoptosis. PARP interacts with proteins in the base excision repair complex containing at least XRCC1, PARP2, POLB and LIG3. In addition PARP forms heterodimers with PARP2, and interacts with PARP3. The 5A5 monoclonal antibody recognizes the N-terminal region of human and mouse PARP and has been shown to be useful for Western blotting and immunofluorescence staining.
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Images (4) Reviews (0) Related Products (27) Exploring PARP Working with PARP
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Jump to: Publications, Images, Ask a Question
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Working with PARP
PARP Antibody (5A5) [Biotin] Images (4) Hela cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-PARP antibody. Proteins were visualized using a donkey anti-mouse secondary antibody conjugated to HRP and a chemiluminescence system. |  Overlay of panels A and B, showing the nuclear localization of PARP |  Hela cells stained with PE-conjugated antibody against PARP |  Hela cells stained with DAPI to indicate nuclei |
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Publications (4)PARP Antibody (5A5) [Biotin] Background References:- 4. Noel, G., et al., 2003. BMC Cell Biol. 4:7.
- Ying, W., et al., 2001. PNAS 98:12227.
more... - Ikejima, M., et al., 1990. J. Biol. Chem. 265:21907.
- Cherney, B., et al., 1987. PNAS 84:8370.
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Jump to: Conjugated Antibodies, Positive Controls, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies
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Related ProductsConjugated AntibodiesPositive Controls| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Other Positive Controls |
Peptides and ProteinsPrimary Antibodies (17)| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| PARP Antibody (C-2-10) | NB100-111 | Bv, Ch(-), Ha, Hu, Mk, Mu, Rt | ELISA, ICC, WB | (1) | (3) | | | | PARP Antibody (E102) | NB110-57323 | Hu, Mu(-), Rt | FACS, ICC, IHC-P, WB | (4) | (1) | | | | PARP Antibody (A6.4.12) | NB100-64828 | Dr, Hu, Mu, Rt, Xp | ELISA, IF, IHC-Fr, IHC-P, IP, WB | (1) | (3) | | | | PARP Antibody | NB100-61616 | Hu | IHC-P, WB | (3) | | | | | PARP Antibody | NSB698 | Bv, Hu | ICC, WB | (2) | (2) | | | | ... and 12 more. See All » |
RNAi (1)Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Mouse IgG Antibody [Biotin] | NB720-B | Mu | ELISA, IHC-P, WB | | | | | | Mouse IgG Antibody [HRP] | NB720-H | Mu | ELISA, ICC, IHC-P, WB | | | | | | Mouse IgG Antibody [Alkaline Phosphatase] | NB720-AP | Mu | ELISA, ICC, IHC-P, WB | | | | | | Mouse IgG Antibody [FITC] | NB720-F | Mu | ICC, IHC-P | | (2) | | | | Mouse IgG Antibody | NB120-7056 | Bv(-), Ch(-), Eq(-), Gp(-), Gt(-), Ha(-), Hu(-), Mu, Rb(-), Rt(-), Sh(-) | ELISA, IHC-P, IP, WB | | | | | | See All » |
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