PARP Antibody (H00000142-M01)

PARP Antibody (3G4)

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PARP Antibody (3G4) Summary:
Species:Hu
Applications:ELISA, IF, WB
Clonality:Monoclonal
Gene:PARP1
Purity:IgG purified
Host:Mouse
Specificity:PARP1 - poly (ADP-ribose) polymerase family, member 1
Publications:Antibody has been mentioned in at least 1 Publication.
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
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PARP Antibody (3G4) Details:
Clone:3G4
Isotype:IgG2a Kappa
Immunogen:PARP1 (AAH37545, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag.
Epitope:MAESSDKLYRVEYAKSGRASCKKCSESIPK DSLRMAIMVQSPMFDGKVPHWYHFSCFWKV GHSIRHPDVEVDGFSELRWDDQQKVKKTAE AGGVTGKGQD
Species Reactivity:

Human. Other species not tested.

Applications:
Uses:Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.
Dilutions:ELISA, immunofluorescence, Western Blot 1:500
Unit Size:0.1 mg
Concentration:Please see the vial label for concentration.
Notes:
This product is produced by and distributed for Abnova, a company based in Taiwan.
Packaging:
Storage:Aliquot and store at -20 °C or -80 °C. Avoid freeze-thaw cycles.
Buffer:In 1x PBS, pH 7.2
Preservative:No Preservative
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: PARP1
Entrez142 (Human)
OMIM173870 (Human)
Swiss ProtAAH37545 (Human)
 
Background:

This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.

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Working with PARP

PARP Antibody (3G4) Images (3)
Immunofluorescence of monoclonal antibody to PARP1 (H00000142-M01) on HeLa cell . [antibody concentration 10 ug/ml]PARP1 monoclonal antibody (M01), clone 3G4 Western Blot analysis of PARP1 expression in HeLa NE ( Cat # L013V3 ).
Detection limit for recombinant GST tagged PARP1 is approximately 0.1ng/ml as a capture antibody.
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Publications (1)

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Jump to: Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies

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PARP in Context


Research Areas related to PARP (7):
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