PARP Antibody (NB110-60963)
PARP Antibody (10H)
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anti-PADPR antibody, anti-adp ribose polymerase antibody |
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PARP Antibody (10H) Summary: | | Gene: | PARP1 |
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| Purity: | Protein A purified |
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| Host: | Mouse |
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| | Specificity: | Recognizes poly(ADP-ribose) polymer (pADPr) synthesized by a variety of poly(ADP-ribose) polymerases (PARP)-related enzymes including PARP-1, -2, -3, tankyrase, vPARP, sPARP and others. Uses for the clone 10H monoclonal antibody include Western blotting, immunostaining, ELISA-based PARP activity screen, and immunodot blot. |
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| Publications: | Antibody has at least 1 Background Reference. |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional PARP Products PARP Antibody (10H) Details: | Clone: | 10H |
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| Isotype: | IgG3 Kappa |
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| Immunogen: | Poly(ADP-ribose) mixed with methylated bovine serum albumin. |
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| Localization: | Nuclear and cytoplasmic |
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Reacts with Poly ADP-ribose polymer. Does not crossreact with ADP-ribose, 5?-AMP, or yeast RNA as tested by ELISA. This antibody crossreacts to bovine serum albumin due to its use as a carrier for the immunogen. |
| Uses: | Western Blot (10 ug/ml using colorimetric
methods). |
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| Dilutions: | ELISA ,
immunohistochemistry 5-20 ug/ml,
Western Blot 2 ug/ml,
Immunocytochemistry 5-20 ug/ml,
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| Unit Size: | 0.2 mg |
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| Concentration: | 1.0 mg/ml |
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| Positive Controls: | 1 Positive Controls |
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Packaging: | Storage: | Aliquot and store at -20 °C or -80 °C. Avoid freeze-thaw cycles. |
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| Buffer: | 20 mM Tris, pH 7.4, 150 mM NaCl, 1% BSA |
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| Preservative: | 0.02% Sodium Azide |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: Poly(ADP-ribose) or "pADPr" is a polymer synthesized by a class of enzymes named poly(ADP-ribose) polymerase's (PARP). Using NAD+ as substrate, PARP catalyzes the formation of the polymer pADPr, with chain lengths ranging from 2 to 300 residues, containing approximately 2% branching in the chain. pADPr becomes attached to nuclear proteins, and to PARP itself (automodification).
Under normal conditions, cells display low basal level of pADPr polymer, which can dramatically increase in cells exposed to DNA damaging agents (irradiation, alkylation, etc.). This increase of polymer synthesis is usually transient and is followed by a rapid degradation phase with a short half life which can be less than 1 min. The low endogenous level of polymer in unstimulated cells and its rapid catabolism during DNA damage has been ascribed to high activity of the polymer catabolizing enzyme poly(ADP-ribose) glycohydrolyase (PARG).
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Images (1) Reviews (0) Related Products (27) Working with PARP
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Jump to: Publications, Images, Ask a Question
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Working with PARP
PARP Antibody (10H) Images (1) Poly(ADP-ribose) staining of rat liver. Rats were injected i.p. with diethylnitrosamine (200 mg/kg), and the livers were removed and rapidly processed 10 hr later, at peak polymer induction (DEN). Control was untreated liver. Tissue were stained according to the above protocol using anti-pADPr clone 10H. (courtesy J.B. Kirkland, Univ. Guelph, Canada) |
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Publications (1)PARP Antibody (10H) Background References:- G.M. Shah et al. (1995) Anal. Biochem. 227, 1D. D
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Jump to: Positive Controls, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies
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Related ProductsPositive Controls| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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Other Positive Controls- Rat liver induced for PADPR synthesis by injection with diethylnitrosamine.
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Peptides and ProteinsPrimary Antibodies (17)| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| PARP Antibody (C-2-10) | NB100-111 | Bv, Ch(-), Ha, Hu, Mk, Mu, Rt | ELISA, ICC, WB | (1) | (3) | | | | PARP Antibody (E102) | NB110-57323 | Hu, Mu(-), Rt | FACS, ICC, IHC-P, WB | (4) | (1) | | | | PARP Antibody (A6.4.12) | NB100-64828 | Dr, Hu, Mu, Rt, Xp | ELISA, IF, IHC-Fr, IHC-P, IP, WB | (1) | (3) | | | | PARP Antibody | NB100-61616 | Hu | IHC-P, WB | (3) | | | | | PARP Antibody | NSB698 | Bv, Hu | ICC, WB | (2) | (2) | | | | ... and 12 more. See All » |
RNAi (1)Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Mouse IgG Antibody [Biotin] | NB720-B | Mu | ELISA, IHC-P, WB | | | | | | Mouse IgG Antibody [HRP] | NB720-H | Mu | ELISA, ICC, IHC-P, WB | | | | | | Mouse IgG Antibody [Alkaline Phosphatase] | NB720-AP | Mu | ELISA, ICC, IHC-P, WB | | | | | | Mouse IgG Antibody [FITC] | NB720-F | Mu | ICC, IHC-P | | (2) | | | | Mouse IgG Antibody | NB120-7056 | Bv(-), Ch(-), Eq(-), Gp(-), Gt(-), Ha(-), Hu(-), Mu, Rb(-), Rt(-), Sh(-) | ELISA, IHC-P, IP, WB | | | | | | See All » |
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