OCT4 Antibody 0.1 ml Embryonic Stem Cell Marker

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Western Blot: OCT4 Antibody [NB100-2379] - Detection of OCT4 in mouse brain lysate.
Immunocytochemistry/Immunofluorescence: OCT4 Antibody [NB100-2379] - OCT4 staining in NTERA-2 cells detected with a Dylight 488 labeled secondary antibody.
Flow Cytometry: OCT4 Antibody [NB100-2379] - Staining of NTERA-2  cells using NB100-2379 at a 1:50 dilution detected using Dylight-488 conjugated goat anti-rabbit secondary antibody.
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OCT4 Antibody Summary

Species Human, Mouse, Rat, Primate
Predicted Species
Porcine (93%), Bovine (93%), Feline (93%). Backed by our 100% Guarantee
Tested Applications WB, Flow, ICC/IF
Immunogen affinity purified
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OCT4 Antibody Details

Embryonic Stem Cell Marker
A synthetic peptide made to an internal portion of the human OCT4 protein (between residues 100-200) [UniProt# Q01860]

Species Reactivity

Human, monkey, rat, mouse. Immunogen has 93% homology to cow, feline and pig. Customer feedback suggests that this antibody does not give good results in IHC-P on feline tissues.
Publications Read Publications using NB100-2379 in the following species:


  • Western Blot 2ug/ml
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
Application Notes
This OCT4 antibody is useful for Immunocytochemistry/Immunofluorescence, Flow Cytometry, and Western blot where a band is seen at ~40 kDa.
Molecular Weight
40 kDa
Positive Controls
Other Available
Alexa Fluor (R) 405 Labeled NB100-2379AF405
Alexa Fluor (R) 488 Labeled NB100-2379AF488
Alexa Fluor (R) 647 Labeled NB100-2379AF647
Alexa Fluor (R) 700 Labeled NB100-2379AF700
Biotin Labeled NB100-2379B
DyLight 405 Labeled NB100-2379V
DyLight 488 Labeled NB100-2379G
DyLight 550 Labeled NB100-2379R
DyLight 650 Labeled NB100-2379C
FITC Labeled NB100-2379F
HRP Labeled NB100-2379H
PE Labeled NB100-2379PE
Control Peptide
OCT4 Peptide (NB100-2379PEP)
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NB100-2379 in the following applications:

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OCT4 Antibody (NB100-2379) has been mentioned in at least 5 publications.
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Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Tris-glycine, 150 mM NaCl
Unit Size
0.1 ml
0.85 mg/ml
0.05% Sodium Azide
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.


Gene Symbol POU5F1

Alternate Names for OCT4 Antibody

  • class 5, transcription factor 1
  • MGC22487
  • Oct-3
  • OCT3Oct4
  • Oct-4
  • Octamer-binding protein 3
  • OTF3POU domain class 5, transcription factor 1
  • OTF4
  • POU class 5 homeobox 1
  • POU-type homeodomain-containing DNA-binding protein
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Related Products by Gene


OCT4 (octamer-binding protein 4) is a transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and generates a trimeric complex with SOX2 on DNA for controlling the expression of several genes (YES1, FGF4, UTF1, ZFP206 etc.) involved in embryonic development. Expressed mainly in the nucleus, OCT4 interacts with UBE2I, ZSCAN10, PKM, WWP2 etc. and OCT4-PKM interaction positively regulate OCT4's transcriptional activity. Once translated, its modifications such as sumoylation enhances the protein stability, DNA binding and transactivation activity and is required for enhanced YES1 expression. OCT4 undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation. OCT4 is found in oocytes and is zygotically expressed in pluripotent cells of pre-gastrulation embryos, and has been observed to be essential for early embryogenesis as well as for embryonic stem cell pluripotency. OCT4 is expressed in developing brain with highest levels in specific cell layers of the cortex, the olfactory bulb, hippocampus and cerebellum. Its expression decreases gradually after embryoid body formation, however, improper re-activation of OCT4 contributes to oncogenic processes and it has been implicated in certain malignancies such as breast and ovarian cancer.

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Publications for OCT4 Antibody (NB100-2379) (5)

Read what people are writing who have used OCT4 Antibody (NB100-2379).

We have publications tested in 3 confirmed species: Human, Rat, Bovine.

We have publications tested in 2 applications: WB, ICC/IF.

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Product General Protocols

View specific protocols for OCT4 Antibody (NB100-2379): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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FAQs for OCT4 (3)

  1. We are looking for the following markers to use to stain cells derived from both human and mouse embryonic and induced pluripotent stem cells: undifferentiated cells, neurons, oligodendrocytes, and astrocytes.
    • For ES markers some popular targets are OCT4 (NB100-2379), KLF4 (NBP2-17070) and SOX2 (NB110-37235). PEA15 is expressed in Astrocytes and this might be one of interest for you (NBP1-61390). OCT4 is one that can also be expressed in Oligodendrocytes.
  2. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
    • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
  3. I was wondering what does Format 7C mean as far as anitbodies go?
    • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)

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Goat Polyclonal
Species Human
Applications WB, ChIP, ICC

     4 Reviews

32 Publications
Western Blot: NANOGP8 Antibody [NBP1-68543] - Analysis of extracts from HuvEc/HT-29/HepG2/Jurkat cells, using NANOGP8 Antibody. The lane on the right is treated with the synthesized peptide.

Rabbit Polyclonal
Species Human
Applications WB, ELISA

Western Blot: SOX2 Antibody [NB110-37235] - Detection of SOX2 in mouse brain lysate using NB110-37235 (0.5ug/ml).Immunocytochemistry/Immunofluorescence: SOX2 Antibody [NB110-37235] - ICC/IF staining of SOX2 in NTERA2 cells with Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained with Dylight 550 (Red) and DAPI (Blue), respectively.Immunohistochemistry-Paraffin: SOX2 Antibody [NB110-37235] - Staining of human uterus, endometrial glands.

Rabbit Polyclonal
Species Human, Mouse, Sheep
Applications WB, Flow, ICC/IF...MORE

     1 Review

7 Publications
Western Blot: c-Myc Antibody (9E10) [NB600-302] - Analysis of c-myc in Jurkat cell lysates using NB600-302.Immunocytochemistry/Immunofluorescence: c-Myc Antibody (9E10) [NB600-302] - HeLa cells stained NB600-302 (Green) detected with DyLight Fluor 488 conjugated anti-mouse IgG secondary antibody.  Nuclei are counterstained with Hoechst 33258 (Blue).Simple Western: c-Myc Antibody (9E10) [NB600-302] - Simple Western lane view shows a specific band for c-Myc in 0.5 mg/ml of Jurkat lysate.  This experiment as performed under reducing conditions using the 12-230 kDa separation system.

Mouse Monoclonal
Species Human, Mouse, Drosophila
Applications WB, Simple Western, ChIP...MORE

     3 Reviews

19 Publications
Western Blot: KLF4 Antibody [NBP2-24749] - Lysate from human 293 cells in the 1) absence and 2) presence of immunizing peptide, 3) mouse NIH 3T3 and 4) RAW cells probed with KLF4 antibody at 1 ug/ml (293) and 2 ug/ml (NIH 3T3 and RAW). I goat anti-rabbit Ig HRP secondary and PicoTect ECL substrate solution were used for this test.Immunohistochemistry-Paraffin: KLF4 Antibody [NBP2-24749] - Breast cancer tissues embedded in paraffin. Negative control is no primary antibody. Image from verified customer review.Flow Cytometry: KLF4 Antibody [NBP2-24749] - Intracellular analysis using KLF4 antibody. Balb/c mouse splenocytes were probed using 1 ug of KLF4 antibody (red) and 1 ug of isotype control was used for this test, and an anti-rabbit IgG PE conjugated secondary.

Rabbit Polyclonal
Species Human, Mouse, Rat...MORE
Applications WB, Flow, ICC/IF...MORE

     1 Review

3 Publications
Western Blot: LIF Antibody (39N7D10) [NBP2-27406] - WB validation of LIF antibody (clone 39N7D10) on (A) full-length recombinant Lif protein, (B) mouse spleen lysate and (C) human spleen lysate. 3 ug/ml concentration of primary antibody, Goat anti-rat Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this assay.Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - IHC analysis of formalin-fixed paraffin-embedded tissue section of mouse brain using 5 ug/ml concentration of LIF antibody (clone 39N7D10).Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal human prostate using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Cell surface/membrane- cytoplasmic immunepositivity  of LIF was observed specifically in the epithelial cells of prostate alveolar glands whereas the surrounding fibromuscular stroma cells did not develop any staining.

Rat Monoclonal
Species Human, Mouse
Applications WB, IHC-P

Western Blot: Rex-1/ZFP42 Antibody (5E11A6) [NBP2-37357] - Western blot analysis using ZFP42 mouse mAb against NIH/3T3 cell lysate.Immunohistochemistry: Rex-1/ZFP42 Antibody (5E11A6) [NBP2-37357] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues using ZFP42 mouse mAb with DAB staining.Flow Cytometry: Rex-1/ZFP42 Antibody (5E11A6) [NBP2-37357] - Flow cytometric analysis of HEK293 cells using ZFP42 mouse mAb (green) and negative control (red).

Mouse Monoclonal
Species Human, Mouse
Applications WB, ELISA, Flow...MORE

Western Blot: SSEA1 Antibody (MC-480) [NB100-1831] - Western blot analysis of Ntera2 cell lysate (A), HUVEC cell lysate (B), Jurkat cell lysate (C), human stomach tissue (D), human brain tissue (E), MEF cell lysate (F), and mouse brain tissue (G) using SSEA 1 antibody (NB100-1831) at 2 ug/ml.Immunocytochemistry/Immunofluorescence: SSEA1 Antibody (MC-480) [NB100-1831] - SSEA1 antibody (0.1ug/ml) was tested in Ntera2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.Flow Cytometry: SSEA1 Antibody (MC-480) [NB100-1831] - Staining of NTERA-2 cells using NB100-1831 at a 1:50 dilution detected using Dylight-488 conjugated goat anti-mouse IgM secondary antibody.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, Flow...MORE

4 Publications
Immunohistochemistry: Nestin Antibody [NB100-1604] - A tissue section through an e 13 mouse brain showing Nestin (green staining) in neural stem cells of the ventricular zone of the CNS. DAPI (blue staining) allows visualization of nuclei.  The fixative was 2% paraformaldehyde in PBS, and the tissue was emersed in this fixative for 30 minutes at room temperature.Immunohistochemistry-Frozen: Nestin Antibody [NB100-1604] - Analysis of Nestin in frozen mouse tissue using anti-Nestin antibody. Image from verified customer review.

Chicken Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC...MORE

     2 Reviews

19 Publications
CD117/c-kit was detected in immersion fixed frozen sections of mouse embryo using Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <A class=NoLineLink href=

Goat Polyclonal
Species Mouse
Applications WB, Flow, IHC

     3 Reviews

7 Publications

Immunohistochemistry: Steroid sulfatase Antibody [NBP1-90095] - Staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.

Rabbit Polyclonal
Species Human
Applications IHC

2 Publications
Western Blot: Lin28 Antibody [NBP1-49537] - Analysis of LIN28 in NTERA-2 cell lysateImmunocytochemistry/Immunofluorescence: Lin28 Antibody [NBP1-49537] - Analysis of LIN28 in NTERA-2 cells using NBP1-49537. Nuclei (Blue) are counterstained using Hoechst 33258.Immunohistochemistry: Lin28 Antibody [NBP1-49537] - Immunohistochemical analysis of LIN28 in E17.5 mouse embryo

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ICC/IF, IHC...MORE

1 Publication
Western Blot: CD44 Antibody (8E2F3) [NBP1-47386] - Western blot analysis using anti-CD44 mAb against Hela (1) and HUVE-12 (2) cell lysates.Immunocytochemistry/Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - Confocal analysis of paraffin-embedded human lung cancer tissues using anti-CD44 mAb (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.Immunohistochemistry-Paraffin: CD44 Antibody (8E2F3) [NBP1-47386] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissues, showing membrane localization with DAB staining using anti-CD44 mAb.

Mouse Monoclonal
Species Human, Mouse, Rabbit (Negative)
Applications WB, ELISA, Flow...MORE

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Mouse Monoclonal
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Bioinformatics Tool for OCT4

Discover related pathways, diseases and genes to OCT4. Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Bioinformatics Tool

Blogs on OCT4

Check out the latest blog posts on OCT4.
Nanog is a Master Controller of ES cell Pluripotency
Nanog, a homeodomain (HD) transcription factor, plays a critical role in the maintenance of embryonic stem (ES) cell self-renewal. Transcription regulator involved in inner cell mass and ES cell proliferation and self-renewal. Imposes pluripotency on...  Read full blog post.

Histones, Bmi1 & OCT4: Investigating the Secrets of ESC Pluripotency
Epigenetic alterations have come to prominence in biomedical research. In particular, hypermethylation of CpG islands located in the promoter regions of tumor-suppressor genes is now firmly established as an important mechanism for gene inactivation i...  Read full blog post.

Sox2 and Oct4: Roles in Embryonic Stem Cell Pluripotency
Embryonic stem (ES) cells are cells derived from the inner cell mass of the blastocyst, an early-stage embryo. ES cells are distinguished from other cells due to their pluripotency, which is the ability to differentiate into any different type of cell...  Read full blog post.

An Unlikely Pairing: The SOX2 Antibody and Breast Cancer
SOX2 encodes a member of the SOX (SRY-related HMG-box) family of transcription factors, which play a vital role in embryonic development. SOX2 antibody research has identified Sox2 as a key transcription factor in pluripotent stem cells. We at Novus B...  Read full blog post.

The Sox2 Antibody Aids Brain Cancer Research
The Sox2 antibody is widely used in sensory, neuroscience and stem cell marker research. Recently, Sox2 antibody preparations identified the Sox2 protein as a marker for malignant neural gliomas. We at Novus Biologicals offer a wide variety of highly...  Read full blog post.

Not as Pluripotent as You Used to Be: Embryonic Stem Cell Markers and the Aging Process
We at Novus Biologicals recently extended our antibody catalog to include several embryonic stem cells (ESC) antibodies validated for use in FACS (fluorescent activated cell sorting) assays. Among them was Oct4, which recently became the focus of an i...  Read full blog post.

Fluorescence Activated Cell Sorting Antibody Techniques
Recently, we at Novus Biologicals added several embryonic stem cell marker products to our antibody catalog, validated for use in fluorescent activated cell sorting (FACS) assays. They included Cripto1, PODXL, SSEA, OCT4, Nanog, SOX2, TRA-1, TERT and...  Read full blog post.