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OCT4 Antibody 0.1ml Embryonic Stem Cell Marker

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Catalog Number  NB100-2379
Sample Size Available
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Jump to Information on OCT4 Antibody (NB100-2379)

OCT4 Antibody Summary
 
Species Human, Mouse, Rat, Bovine, Primate
Predicted Species Feline (93%), Porcine (93%). Backed by our 100% guarantee.
Tested Applications WB, FLOW, ICC/IF
Clonality Polyclonal
Host Rabbit
Gene POU5F1
Purity Immunogen affinity purified
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OCT4 Antibody Details
 
Marker Embryonic Stem Cell Marker
Immunogen A synthetic peptide made to an internal portion of the human OCT4 protein (between residues 100-200) [UniProt# Q01860]
Localization Nuclear
Species Reactivity
 
Human, monkey, rat, mouse and bovine. Immunogen has 93% homology to cow, feline and pig. Customer feedback suggests that this antibody does not give good results in IHC-P on feline tissues.
Publications Read 3 using NB100-2379 in the following species:
 
Applications/Dilutions
 
Dilutions
  • Western Blot 2ug/ml
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
Application Notes

This OCT4 antibody is useful for Immunocytochemistry/Immunofluorescence, Flow Cytometry, and Western blot where a band is seen at ~40 kDa.
Molecular Weight

40 kDa
Positive Controls

2 Positive Controls
Directly Conjugated
Formats
DyLight 550 Labeled NB100-2379R
DyLight 488 Labeled NB100-2379G
HRP Labeled NB100-2379H
DyLight 650 Labeled NB100-2379C
Biotin Labeled NB100-2379B
Learn about Custom Antibody Labeling
Control Peptide
OCT4 Peptide (NB100-2379PEP)
Publications
Read 3 publications using
NB100-2379 in the following applications:
  • WB2publications
Product PDFs
  Download PDF Datasheet
  Request CofA
  Download Protocol
  Request MSDS
Reviews
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Publications
OCT4 Antibody (NB100-2379) has been mentioned in at least 4 publications.
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Protocols and FAQs
Controls & Support Products
View all related OCT4 Controls and Support Products.
Research Tools
View all OCT4 Research Tools.
Packaging, Storage & Formulations
 
Storage Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer Tris-glycine, 150 mM NaCl
Unit Size 0.1ml (also available in 0.025ml (sample size))
Concentration 0.85 mg/ml
Preservative 0.05% Sodium Azide
Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 6 months from date of receipt.
Bioinformatics
 
Gene Symbol POU5F1
Entrez
Uniprot
Alternate Names for OCT4
 
  • POU-type homeodomain-containing DNA-binding protein
  • Oct-3
  • class 5, transcription factor 1
  • Octamer-binding protein 3
  • OTF4
  • MGC22487
  • OTF3POU domain class 5, transcription factor 1
  • OCT3Oct4
  • Oct-4
  • POU class 5 homeobox 1
Related Products by Gene
 
NANOG, NANOGP8, SOX2, MYC, KLF4, OCT3/4, LIF, ZFP42, FUT4, NES, KIT, FGF4, STS, OCT-3/4, LIN28A, PROM1, CD44, FGF2, CTLA4, HLA-DQA1
Background
 
OCT4 (octamer-binding protein 4) is a transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and generates a trimeric complex with SOX2 on DNA for controlling the expression of several genes (YES1, FGF4, UTF1, ZFP206 etc.) involved in embryonic development. Expressed mainly in the nucleus, OCT4 interacts with UBE2I, ZSCAN10, PKM, WWP2 etc. and OCT4-PKM interaction positively regulate OCT4's transcriptional activity. Once translated, its modifications such as sumoylation enhances the protein stability, DNA binding and transactivation activity and is required for enhanced YES1 expression. OCT4 undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation. OCT4 is found in oocytes and is zygotically expressed in pluripotent cells of pre-gastrulation embryos, and has been observed to be essential for early embryogenesis as well as for embryonic stem cell pluripotency. OCT4 is expressed in developing brain with highest levels in specific cell layers of the cortex, the olfactory bulb, hippocampus and cerebellum. Its expression decreases gradually after embryoid body formation, however, improper re-activation of OCT4 contributes to oncogenic processes and it has been implicated in certain malignancies such as breast and ovarian cancer.