OCT4 Antibody Summary
||Human, Mouse, Rat, Primate
Feline (93%), Porcine (93%), Bovine (93%). Backed by our 100% Guarantee
||WB, FLOW, ICC/IF
Immunogen affinity purified
All of our products are backed by our 100% guarantee to work for stated and predicted species and applications.
Test in a species/application not listed above to receive a full credit towards a future purchase.
OCT4 Antibody Details
Embryonic Stem Cell Marker
A synthetic peptide made to an internal portion of the human OCT4 protein (between residues 100-200) [UniProt# Q01860]
Human, monkey, rat, mouse. Immunogen has 93% homology to cow, feline and pig. Customer feedback suggests that this antibody does not give good results in IHC-P on feline tissues.
||Read Publications using NB100-2379 in the following species:
- Western Blot 2ug/ml
- Flow Cytometry 1:50-1:200
- Immunocytochemistry/Immunofluorescence 1:50-1:200
This OCT4 antibody is useful for Immunocytochemistry/Immunofluorescence, Flow Cytometry, and Western blot where a band is seen at ~40 kDa.
Read Publications using
NB100-2379 in the following applications:
Frequently Purchased Together
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OCT4 Antibody (NB100-2379) has been mentioned in at least 4 publications
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Packaging, Storage & Formulations
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Tris-glycine, 150 mM NaCl
0.05% Sodium Azide
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
Alternate Names for OCT4 Antibody
- class 5, transcription factor 1
Related Products by Gene
OCT4 (octamer-binding protein 4) is a transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and generates a trimeric complex with SOX2 on DNA for controlling the expression of several genes (YES1, FGF4, UTF1, ZFP206 etc.) involved in embryonic development. Expressed mainly in the nucleus, OCT4 interacts with UBE2I, ZSCAN10, PKM, WWP2 etc. and OCT4-PKM interaction positively regulate OCT4's transcriptional activity. Once translated, its modifications such as sumoylation enhances the protein stability, DNA binding and transactivation activity and is required for enhanced YES1 expression. OCT4 undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation. OCT4 is found in oocytes and is zygotically expressed in pluripotent cells of pre-gastrulation embryos, and has been observed to be essential for early embryogenesis as well as for embryonic stem cell pluripotency. OCT4 is expressed in developing brain with highest levels in specific cell layers of the cortex, the olfactory bulb, hippocampus and cerebellum. Its expression decreases gradually after embryoid body formation, however, improper re-activation of OCT4 contributes to oncogenic processes and it has been implicated in certain malignancies such as breast and ovarian cancer.
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Publications for OCT4 Antibody (NB100-2379) (4)
Read what people are writing who have used OCT4 Antibody (NB100-2379).
We have publications tested in 3 confirmed species: Human, Rat, Bovine.
We have publications tested in 2 applications: WB, ICC/IF.
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|Yenamandra SP, Darekar SD, Kashuba V et al. Stem cell gene expression in MRPS18-2-immortalized rat embryonic fibroblasts. Cell Death Dis 2012 Jan [PMID: 22258410] (WB, Rat)
|Ruggeri RR, Watanabe Y, Meirelles F et al. The use of parthenotegenetic and IVF bovine blastocysts as a model for the creation of human embryonic stem cells under defined conditions J Assist Reprod Genet 2012 Sep 29 [PMID: 23054358] (ICC/IF, Bovine)
|Cho YM, Kim YS, Kang MJ et al. Long-term recovery of irradiated prostate cancer increases cancer stem cells Prostate 2012 Dec 1 [PMID: 22513891] (WB, Human)
|Liu JW, Hsu YC, Kao CY et al. LIF-induced Stat3 signaling suppresses FGF1-induced Erk1/2 activation to inhibit the downstream differentiation in mouse embryonic stem cells Stem Cells Dev 2012 Dec 4 [PMID: 23205673]
Product General Protocols
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FAQs for OCT4 (3)
- We are looking for the following markers to use to stain cells derived from both human and mouse embryonic and induced pluripotent stem cells: undifferentiated cells, neurons, oligodendrocytes, and astrocytes.
- For ES markers some popular targets are OCT4 (NB100-2379), KLF4 (NBP2-17070) and SOX2 (NB110-37235). PEA15 is expressed in Astrocytes and this might be one of interest for you (NBP1-61390). OCT4 is one that can also be expressed in Oligodendrocytes.
- In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
- For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
- I was wondering what does Format 7C mean as far as anitbodies go?
- 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)
Bioinformatics Tool for OCT4
Discover related pathways, diseases and genes to OCT4. Need help? Read the Bioinformatics Tool Guide
for instructions on using this tool.
Diseases for OCT4
Discover more about diseases related to OCT4.
Pathways for OCT4
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Research Areas for OCT4
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PTMs for OCT4
Learn more about PTMs related to OCT4.
Blogs on OCT4
Check out the latest blog posts on OCT4.
Nanog is a Master Controller of ES cell Pluripotency
Nanog, a homeodomain (HD) transcription factor, plays a critical role in the maintenance of embryonic stem (ES) cell self-renewal. Transcription regulator involved in inner cell mass and ES cell proliferation and self-renewal. Imposes pluripotency on ... Read full blog post.
Histones, Bmi1 & OCT4: Investigating the Secrets of ESC Pluripotency
Epigenetic alterations have come to prominence in biomedical research. In particular, hypermethylation of CpG islands located in the promoter regions of tumor-suppressor genes is now firmly established as an important mechanism for gene inactivation i... Read full blog post.
Sox2 and Oct4: Roles in Embryonic Stem Cell Pluripotency
Embryonic stem (ES) cells are cells derived from the inner cell mass of the blastocyst, an early-stage embryo. ES cells are distinguished from other cells due to their pluripotency, which is the ability to differentiate into any different type of cell... Read full blog post.
An Unlikely Pairing: The SOX2 Antibody and Breast Cancer
SOX2 encodes a member of the SOX (SRY-related HMG-box) family of transcription factors, which play a vital role in embryonic development. SOX2 antibody research has identified Sox2 as a key transcription factor in pluripotent stem cells. We at Novus B... Read full blog post.
The Sox2 Antibody Aids Brain Cancer Research
The Sox2 antibody is widely used in sensory, neuroscience and stem cell marker research. Recently, Sox2 antibody preparations identified the Sox2 protein as a marker for malignant neural gliomas. We at Novus Biologicals offer a wide variety of highly ... Read full blog post.
Not as Pluripotent as You Used to Be: Embryonic Stem Cell Markers and the Aging Process
We at Novus Biologicals recently extended our antibody catalog to include several embryonic stem cells (ESC) antibodies validated for use in FACS (fluorescent activated cell sorting) assays. Among them was Oct4, which recently became the focus of an i... Read full blog post.
Fluorescence Activated Cell Sorting Antibody Techniques
Recently, we at Novus Biologicals added several embryonic stem cell marker products to our antibody catalog, validated for use in fluorescent activated cell sorting (FACS) assays. They included Cripto1, PODXL, SSEA, OCT4, Nanog, SOX2, TRA-1, TERT and ... Read full blog post.