OCT4 Antibody 0.1 ml Embryonic Stem Cell Marker

OCT4 NB100-2379

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Ordering Information: NB100-2379

Catalog NumberNB100-2379
  • FormUnconjugated
  • Formats
  • Size(s)
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OCT4 Antibody Summary

 
Species Human, Mouse, Rat, Primate
Predicted Species Bovine (93%), Feline (93%), Porcine (93%). Backed by our 100% guarantee.
Tested Applications WB, FLOW, ICC/IF
Clonality Polyclonal
Host Rabbit
Gene POU5F1
Purity Immunogen affinity purified
Guarantee Plus All of our products are backed by our 100% guarantee to work for stated and predicted species and applications.
Innovator's Reward Test in a species/application not listed above to receive a full credit towards a future purchase.
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OCT4 Antibody Details

 
Marker Embryonic Stem Cell Marker
Immunogen A synthetic peptide made to an internal portion of the human OCT4 protein (between residues 100-200) [UniProt# Q01860]
Localization Nuclear

Species Reactivity

 
Human, monkey, rat, mouse. Immunogen has 93% homology to cow, feline and pig. Customer feedback suggests that this antibody does not give good results in IHC-P on feline tissues.
Publications Read 3 using NB100-2379 in the following species:
 

Applications/Dilutions

 
Dilutions
  • Western Blot 2ug/ml
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
Application Notes

This OCT4 antibody is useful for Immunocytochemistry/Immunofluorescence, Flow Cytometry, and Western blot where a band is seen at ~40 kDa.

Molecular Weight

40 kDa

Positive Controls

2 Positive Controls

Other Available
Formats
Alexa Fluor (R) 405 Labeled NB100-2379AF405
Alexa Fluor (R) 488 Labeled NB100-2379AF488
Alexa Fluor (R) 647 Labeled NB100-2379AF647
Alexa Fluor (R) 700 Labeled NB100-2379AF700
Biotin Labeled NB100-2379B
DyLight 405 Labeled NB100-2379V
DyLight 488 Labeled NB100-2379G
DyLight 550 Labeled NB100-2379R
DyLight 650 Labeled NB100-2379C
FITC Labeled NB100-2379F
HRP Labeled NB100-2379H
PE Labeled NB100-2379PE
Learn about Custom Antibody Labeling
Control Peptide
OCT4 Peptide (NB100-2379PEP)
Publications
Read 3 publications using
NB100-2379 in the following applications:
  • WB2publications

Product PDFs

Reviews

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Publications

OCT4 Antibody (NB100-2379) has been mentioned in at least 4 publications.
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Protocols and FAQs

Controls & Support Products

Research Tools

View all OCT4 Research Tools.

Packaging, Storage & Formulations

 
Storage Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer Tris-glycine, 150 mM NaCl
Unit Size 0.1 ml (also available in 0.025 ml (sample size))
Concentration 0.85 mg/ml
Preservative 0.05% Sodium Azide
Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

 
Gene Symbol POU5F1
Entrez
Uniprot

Alternate Names for OCT4

 
  • POU-type homeodomain-containing DNA-binding protein
  • Oct-3
  • class 5, transcription factor 1
  • Octamer-binding protein 3
  • OTF4
  • MGC22487
  • OTF3POU domain class 5, transcription factor 1
  • OCT3Oct4
  • Oct-4
  • POU class 5 homeobox 1

Related Products by Gene

Background

 
OCT4 (octamer-binding protein 4) is a transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and generates a trimeric complex with SOX2 on DNA for controlling the expression of several genes (YES1, FGF4, UTF1, ZFP206 etc.) involved in embryonic development. Expressed mainly in the nucleus, OCT4 interacts with UBE2I, ZSCAN10, PKM, WWP2 etc. and OCT4-PKM interaction positively regulate OCT4's transcriptional activity. Once translated, its modifications such as sumoylation enhances the protein stability, DNA binding and transactivation activity and is required for enhanced YES1 expression. OCT4 undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation. OCT4 is found in oocytes and is zygotically expressed in pluripotent cells of pre-gastrulation embryos, and has been observed to be essential for early embryogenesis as well as for embryonic stem cell pluripotency. OCT4 is expressed in developing brain with highest levels in specific cell layers of the cortex, the olfactory bulb, hippocampus and cerebellum. Its expression decreases gradually after embryoid body formation, however, improper re-activation of OCT4 contributes to oncogenic processes and it has been implicated in certain malignancies such as breast and ovarian cancer.