Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
Synthetic peptide, C-RKEQERSFRITFD, representing the C Terminus of the human protein according to NP_036478.2; NP_057637.2; NP_714940.1; NP_714941.1
Epitope:
RKEQERSFRITFD
Species Reactivity:
Human, Dog, Cow
Applications:
Uses:
Peptide ELISA: antibody detection limit dilution 1:8000. Western Blot: Preliminary experiments gave an approx 30kDa band in Human Testis lysates after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 53.3kDa according to NP_036478.2, NP_057637.2, NP_714940.1 and NP_714941.1. The 30kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Dilutions:
Peptide ELISA 1:64000
Unit Size:
0.1 mg
Concentration:
0.5 mg/ml
Notes:
Please note, this antibody is considered “Innovators Grade”. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Under this program, Novus will provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal value in exchange for your new data. Please contact us at innovators@novusbio.com for more details.
Packaging:
Storage:
Aliquot and store at -20 °C or -80 °C. Avoid freeze-thaw cycles.
Buffer:
Tris-saline [ pH7.3] with 0.5% BSA
Preservative:
0.02% Sodium Azide
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
The nuclear pore complex is a structure that extends across the nuclear envelope and regulates the flow of macromolecules between the cytoplasm and the nucleus. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. Nup 62 is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. There are multiple transcript variants of this gene, however, they encode a single protein isoform. This protein undergoes post-translational modifications. It contains about 10 N-acetylglucosamine side chain sites.