Nuclear Receptor SHP Antibody

Species: Hu Applications: IHC-P Clonality: Polyclonal	Gene: NR0B2 Purity: Affinity purified Host: Rabbit
Specificity: Nuclear Receptor SHP
See All Nuclear Receptor SHP Products See All Nuclear Receptor SHP Related Products: Lysates Peptides and Proteins Primary Antibodies RNAi
*Note not all species have been tested for reactivity. Only those species listed have been tested. We cannot make any guarantees about additional reactivities or cross reactivities beyond those that have been tested. This antibody may or may not react with other species.

Immunogen:	Synthetic peptide [KLH conjugated] made to the ligand binding domain
Epitope:	ligand-binding domain of h uman

Entrez:	8431 (Human)
Related Genes:	NR1H4, CYP7A1, NR5A2, PTPN6, PTPN11, ABCB11, HNF4A, SRC, NR0B1, P2RX5, NR5A1, INS, NR1H3, CYP8B1, SREBF1, MLX, NR1I3, NR1I2, HNF1A, NCOA1, JUN, TNF, SLC10A1, NR1H2, PRL2C3, CYP27A1, FGF15, ESR1, MAPK1, SLCO1B2, PPARA, CTSD, HLA-DQA1, MAPK3, NOD2, LRP6, PCK2, PPARG, ABCC3, SLC10A2

SHP (short heterodimer partner) is a bile acid-dependent orphan NR0 Knirps-Like receptor with dimerization and ligand-binding domains but not the conventional DNA-binding domain. SHP has been shown to inhibit the transcriptional activity of other nuclear receptors, including thyroid hormone receptor, constitutive androstane receptor, and retinoic acid receptors. SHP affects hepatic cholesterol catabolism based on a two-step mechanism dependent on both coactivator competition and direct transcriptional repression by mediating the repression of CYP7A1, the rate-limiting enzyme for bile acid synthesis. Mutations in the SHP gene contribute to increased body weight, indicating a possible role of SHP in the development of early-onset diabetes (maturity-onset diabetes of the young, MODY). Loss of SHP in mice caused abnormal accumulation and increased synthesis of bile acids due to derepression of rate-limiting CYP7A1 and CYP8B1 hydroxylase enzymes in the biosynthetic pathway. SHP expression has been documented in human liver, adrenal, spleen, and pancreas. ESTs have been isolated from cancerous human kidney, lung, and stomach, as well as normal human heart, kidney, liver, and liver/spleen. Caution: The nuclear receptor SHP should not be confused with the tyrosine phosphatases SHP-1 and SHP-2.

Reactivity:	Human.

Uses:	Immunohistochemistry with formalin fixed paraffin embedded tissues. Optimal working dilutions should be determined by investigator.
Dilutions:	Immunohistochemistry-Paraffin assay dependent
Concentrations:	1.0 mg/ml
Positive Controls:	1 positive controls

Buffer:	Phosphate buffered saline, PH 7.7
Storage:	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Preservative:	0.01% Sodium Azide
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.