Niemann-Pick C1 Antibody (EPR5209) Summary
| Immunogen |
A synthetic peptide corresponding to residues at the C-terminus of human NPC1 was used as an immunogen. |
| Clonality |
Monoclonal |
| Host |
Rabbit |
| Gene |
NPC1 |
| Purity |
Tissue culture supernatant |
| Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
| Dilutions |
- Immunocytochemistry/ Immunofluorescence 1:100 - 250
- Immunohistochemistry
- Immunohistochemistry-Paraffin 1:50 - 100
- Western Blot 1:1000 - 10000
|
Reactivity Notes
Human, Mouse, Rat. Cross reactivity determined by Western Blot only.
Packaging, Storage & Formulations
| Storage |
Store at -20C. Avoid freeze-thaw cycles. |
| Buffer |
49% PBS, 0.05% BSA and 50% Glycerol |
| Preservative |
0.01% Sodium Azide |
| Purity |
Tissue culture supernatant |
Notes
Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Alternate Names for Niemann-Pick C1 Antibody (EPR5209)
Background
NPC1 is a large protein that resides in the limiting membrane of endosomes and lysosomes and mediates intracellular cholesterol trafficking via binding of cholesterol to its N-terminal domain. It is predicted to have a cytoplasmic C-terminus, 13 transmembrane domains, and 3 large loops in the lumen of the endosome - the last loop being at the N-terminus. NPC1 transports low-density lipoproteins to late endosomal/lysosomal compartments where they are hydrolized and released as free cholesterol. Defects in this protein cause Niemann-Pick type C disease, a rare autosomal recessive neurodegenerative disorder characterized by overaccumulation of cholesterol and glycosphingolipids in late endosomal/lysosomal compartments (1).
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Publications for Niemann-Pick C1 Antibody (NBP1-95817) (0)
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Product General Protocols
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Video Protocols
FAQs for Niemann-Pick C1 Antibody (NBP1-95817). (Showing 1 - 1 of 1 FAQ).
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I'm looking for a Niemann-Pick type1 or 2 antibody to stain mouse heart section embedded in paraffin. I would like to label these sections with another primary antibody to show colocalisation of my protein of interest. I would like to know if you have a rabbit anti-Niemann-Pick antibody that works well for fluorescence immunohistochemistry?
- Niemann-Pick C1 Antibody (<a href="http://www.novusbio.com/NB400-148" target="_self">NB400-148</a>) (0.1 ml) is one of our best sellers and this antibody has been cited in at least 17 peer reviewed research publications in journals of high repute. We have only one Niemann-Pick type C2 antibody with catalog # <a href="http://www.novusbio.com/H00010577-D01P" target="_self">H00010577-D01P</a>, but this antibody is yet to be established for IHC application. However, if you would like to try this antibody for IHC-P application you would qualify for our Innovators Reward program. Through this program if you complete an online review with image, detailing your positive or negative results we will send you a discount voucher for 100% of the purchase price of the reviewed product. Read more about <a href="http://www.novusbio.com/support/innovators-reward.html" target="_self">Innovators Reward Program</a>.