Neuropeptide Y Antibody (NBP1-78352)

Neuropeptide Y Antibody (NBP1-78352) (100 ul)

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	Catalog Number	NBP1-78352
	Size(s)	100 ul	Price:
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Neuropeptide Y Antibody Summary:

Species:	Hu, Mu, Rt, Av, Fe, Fi, GP, Other, Ze	Gene:	NPY
Applications:	ICC, IHC-P	Purity:	Whole antisera
Clonality:	Polyclonal	Host:	Rabbit
Publications:

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Neuropeptide Y Antibody Details:

Isotype:	IgG
Immunogen:	Neuropeptide Y; coupled to bovine thyroglobulin (BTg) with glutaraldehyde

Species Reactivity:

Reacts with Newt, Bird, Rat, Mouse, Human,Cat, Guinea Pig, Zebra Fish, Squirrels, Frog

Applications:

Uses:	In rat central nervous system, the antiserum has significant staining with a very low background at a 1/5,000 – 1/10,000 dilution using the Biotin-Streptavidin/HRP detection method. All staining is blocked by pre-absorption of the diluted antiserum with excess NPY. Cross reactivity experiments in which diluted NPY antiserum was absorbed with excess peptide YY, avian pancreatic polypeptide, B-endorphin, vasoactive intestinal peptide, cholecystokinin or somatostatin showed no affect in blocking the intensity of staining.
Dilutions:	Immunocytochemistry, Immunohistochemistry-Paraffin
Unit Size:	100 ul
Concentration:	Lyoph

Notes:

Fixative: 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4; 500 mL over ~ 20-30 min. Post Fixation: 1.5 hr. at 4C in 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4.

Packaging:

Storage:	Store at -20°C. Avoid freeze-thaw cycles.
Buffer:	Lyophilized whole serum
Preservative:	0.09% Sodium Azide
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Gene Symbol: NPY

Uniprot:	P01304 (Human)

Related Genes:

Background:

Neuropeptide Y (NPY) is a member of a regulatory peptide family and has marked sequence homology with pancreatic polypeptide (PP) and peptide YY (PYY), which are other members of the family. In the rat central nervous system, immunohistochemistry has foun

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Neuropeptide Y Antibody Images (1)

Immunohistochemistry-Paraffin: Neuropeptide Y Antibody [NBP1-78352] In the rat central nervous system, immunohistochemistry has found NPY-like cell bodies in the cortex, caudate-putamen, hypothalamus (arcuate nucleus), hippocampus, anterior olfactory bulb, nucleus accumbens, amygdaloid complex and periaqueductal grey. NPY-like fibers and terminals are detected in high numbers in the bed nucleus of the stria terminalis, the peri- and paraventricular regions of the hypothalamus and thalamus and in discrete hypothalamic nuclei, particularly the suprachiasmatic nucleus.