- Products
- Research Areas
- Epigenetics
- Amino Acids, Drugs and other small molecules
- Apoptosis
- Autophagy
- Cancer
- Cell Biology
- Cell Cycle and Replication
- Cellular Markers
- Cytoskeleton
- DNA Repair
- Epitope Tags
- GPCR
- Hypoxia
- Immunology
- Lipid and Metabolism
- Loading Controls
- MAP Kinase Signaling
- Membrane Trafficking and Chaperones
- Neuroscience
- Nuclear Receptors, Coactivators and Corepressors
- Phospho-Specific
- Protein Kinase
- Protein Phosphatase
- Protein Turnover
- Signal Transduction
- Stem Cell Markers
- Transcription Factors and Regulators
- Translation Control
- Tyrosine Kinases
- Wnt Signaling Pathway
- modENCODE Antibodies
- Support
- Distributors
- About
Support: 303-730-1950
Worldwide (Choose your country.)
![Western Blot: Nestin Antibody [NB600-876] - Western blot using Affinity Purified anti-Nestin antibody shows detection of a band ~220 kDa corresponding to mouse Nestin (arrowhead). Approximately 30 ìg of MEF whole cell lysate was separated by SDS-PAGE using a 4-20% gradient gel. After transfer onto nitrocellulose, the membrane was blocked and then probed with the primary antibody diluted to 1:2,000 overnight at 4 degrees C. The membrane was then washed and reacted with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (611- 132-122) for 45 min at room temperature. IRDye800 fluorescence image was captured using the OdysseyInfrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](http://images.novusbio.com/images/Nestin Antibody-Western Blot-NB600-876-img0001.jpg "Western Blot: Nestin Antibody [NB600-876] - Western blot using Affinity Purified anti-Nestin antibody shows detection of a band ~220 kDa corresponding to mouse Nestin (arrowhead). Approximately 30 ìg of MEF whole cell lysate was separated by SDS-PAGE using a 4-20% gradient gel. After transfer onto nitrocellulose, the membrane was blocked and then probed with the primary antibody diluted to 1:2,000 overnight at 4 degrees C. The membrane was then washed and reacted with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (611- 132-122) for 45 min at room temperature. IRDye800 fluorescence image was captured using the OdysseyInfrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.")
![Western Blot: Nestin Antibody [NB600-876] - 1A human brain cortical neuron neuronal progenitor lysate (lane 1), or differentiated HCN-1A human brain cortical neuron neuronal progenitor lysate (lane 2) were separated by SDS-PAGE using 4-20% gradient gel. After transfer onto nitrocellulose, the membrane was blocked and then probed with the primary antibody diluted to 1:2,000 overnight at 4C. The membrane was then washed and reacted with a 1:10,000 dilution of peroxidase conjugated affinity purified Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature. Image was captured using film.](http://images.novusbio.com/images/Nestin Antibody-Western Blot-NB600-876-img0002.jpg "Western Blot: Nestin Antibody [NB600-876] - 1A human brain cortical neuron neuronal progenitor lysate (lane 1), or differentiated HCN-1A human brain cortical neuron neuronal progenitor lysate (lane 2) were separated by SDS-PAGE using 4-20% gradient gel. After transfer onto nitrocellulose, the membrane was blocked and then probed with the primary antibody diluted to 1:2,000 overnight at 4C. The membrane was then washed and reacted with a 1:10,000 dilution of peroxidase conjugated affinity purified Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature. Image was captured using film.")
