Nestin Antibody (NB100-1604) | Novus Biologicals

Nestin Antibody (NB100-1604) (0.25 ml)

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	Catalog Number	NB100-1604
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anti-FLJ21841 antibody, anti-nestin antibody

Nestin Antibody Summary:

Species:	Mu	Gene:	NES
Applications:	WB, ICC, IHC-P	Purity:	Affinity purified
Clonality:	Polyclonal	Host:	Chicken
Specificity:	Nestin - Neuronal Stem Cell Marker
Publications:	NB100-1604 has been mentioned in at least 5 Publications

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Nestin Antibody Details:

Isotype:	IgY
Immunogen:	Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the mouse nestin gene product (NP_057910).
Localization:	Cytoplasmic
Marker:	Neuronal Stem Cell Marker, Cytoskeleton-Intermediate Filaments Marker

Species Reactivity:

NB 100-1604 reacts with mouse nestin, but the immunogen regions are shared with the rat protein sequence (NM_012987). Other species have not been tested.

Applications:

Uses:	This antibody is useful for Immunohistochemistry-Paraffin and Western blot.
Dilutions:	Immunocytochemistry 1:10000-1:20000, Immunohistochemistry-Paraffin 1:10000-1:20000, Western Blot 1:20000-1:50000
Unit Size:	0.25 ml (also available: 1 ml)
Concentration:	0.3 mg/ml
Positive Controls:	1 Positive Controls

Packaging:

Storage:	Store at 4°C in the dark.
Buffer:	10mM PBS pH7.2
Preservative:	0.02% Sodium Azide
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Gene Symbol: NES

Entrez:	18008 (Mouse)
Uniprot:	Q6P5H2 (Mouse)

Related Genes:

Background:

Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors.

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