Nanog Antibody Summary
WB, Flow, ICC/IF, IP
Immunogen affinity purified
NB100-588 is specific for the Nanog protein.
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Nanog Antibody Details
Embryonic Stem Cell Marker
A synthetic peptide which maps to a region between residues 250 and the C-terminus (residue 305) of mouse Nanog homeobox using the numbering given in TrEMBL entry Q80Z64 (GeneID 71950).
Based on 100% sequence identity, this antibody is predicted to react with Southeastern Asian house mouse, Western Mediterranean mouse and Japanese house mouse.
3 rated 4
using NB100-588 in the following species:
Publications using NB100-588 in the following species:
Western Blot 1:1000-1:10000 Flow Cytometry 1:150 Immunocytochemistry/Immunofluorescence 1:10-1:2000 Immunoprecipitation 2-5 ug/mg lysate
Publications using NB100-588 in the following applications:
Frequently Purchased Together
WB Tested In Human
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Packaging, Storage & Formulations
Store at 4C. Do not freeze.
Tris-citrate/phosphate buffer, pH 7 to 8
0.09% Sodium Azide
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
for 1 year from date of receipt.
Alternate Names for Nanog Antibody
FLJ12581 FLJ40451 hNanog homeobox protein NANOG Homeobox transcription factor Nanog Show More Related Products by Gene
Nanog is one of the molecular markers suitable for recognizing the undifferentiated state of stem cells in the mouse and human. It is a divergent homeodomain protein that directs propagation of undifferentiated ES cells. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Nanog-deficient ES cells lose pluripotency and differentiate into extraembryonic endoderm lineage.
Nanog Antibody (NB100-588) (3)
Read what people are saying who have used Nanog Antibody (NB100-588).
We have 3 reviews tested in 2 species: Human, Mouse.
We have 3 reviews tested in 2: Western Blot, Flow Cytometry.
Average Rating: 4
(Based on 3 reviews)
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Application Western Blot Sample Tested Human cancer cell whole cell lysate Species Human Blocking
Blocking Details Blocking with 5% non-fat milk for 30 min Primary Anitbody
Dilution Ratio 1:1000, overnight Secondary Antibody
Secondary Manufacturer Cat# GE Healthcare, NA934V Secondary Concentration 1:2000 Details
Detection Notes Regular Western blot Protocol
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Application Flow Cytometry Species Mouse
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Application Western Blot Sample Tested HUVEC, MCF7, JURKAT, Sample Amount: 2-30 ug Species Human Pub Med ID 21119109 Blocking
Blocking Details Blocking Buffer: 5% milk in TBS, Blocking Time: 1 hour, Blocking Temp: 25 degrees Celsius Primary Anitbody
Dilution Ratio Dilution Ratio: 8 ug/ml, Incubation Dilution Buffer: 3% BSA in TBS, Incubation Time: overnight/2 hours, Incubation Temp: 4/25C Details
Detection Notes Detection Method: Chemiluminescence, Exposure Time: 3 minutes, Specific Bands: 35 kDa
Publications for Nanog Antibody (NB100-588) (6)
Read what people are writing who have used Nanog Antibody (NB100-588).
We have publications tested in 2 confirmed species: Human, Mouse. We have publications tested in 2 applications: WB, ICC/IF.
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Ganuza M, Saiz-Ladera C, Canamero M, Gomez G, Schneider R, Blasco MA, Pisano D, Paramio JM, Santamaria D, Barbacid M. Genetic inactivation of Cdk7 leads to cell cycle arrest and induces premature aging due to adult stem cell exhaustion. EMBO J. 2012 Apr 13. [PMID: 22505032] (ICC/IF, Mouse) ICC/IF
Kohler EE, Cowan CE, Chatterjee I et al. NANOG induction of fetal liver kinase-1 (FLK1) transcription regulates endothelial cell proliferation and angiogenesis Blood 2011 Feb 3 [PMID: 21119109] (WB, Human) WB
Carey BW, Markoulaki S, Hanna J et al. Reprogramming of murine and human somatic cells using a single polycistronic vector. Proc Natl Acad Sci U S A 2009 Jan [PMID: 19109433]
Wu Y, Guo Z, Wu H et al. SUMOylation represses Nanog expression via modulating transcription factors Oct4 and Sox2. PLoS One 2012 [PMID: 22745796]
Hamada M, Malureanu LA, Wijshake T et al. Reprogramming to pluripotency can conceal somatic cell chromosomal instability. PLoS Genet 2012 [PMID: 22952451]
Morgado-Palacin L, Llanos S, Serrano M Ribosomal stress induces L11- and p53-dependent apoptosis in mouse pluripotent stem cells. Cell Cycle. 2012 Feb 1 [PMID: 22262176] (ICC/IF, Mouse) ICC/IF
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FAQs for Nanog (2)
In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit:
IHC-P Protocol and IHC-P Troubleshooting. I was wondering what does Format 7C mean as far as anitbodies go?
7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)
Bioinformatics Tool for Nanog
Discover related pathways, diseases and genes to Nanog. Need help? Read the
Bioinformatics Tool Guide
for instructions on using this tool.
Diseases for Nanog
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PTMs for Nanog
Learn more about PTMs related to Nanog.
Blogs on Nanog
Check out the latest blog posts on Nanog.
Nanog is a Master Controller of ES cell Pluripotency
Nanog, a homeodomain (HD) transcription factor, plays a critical role in the maintenance of embryonic stem (ES) cell self-renewal. Transcription regulator involved in inner cell mass and ES cell proliferation and self-renewal. Imposes pluripotency on... Read full blog post.
Sox2 and Oct4: Roles in Embryonic Stem Cell Pluripotency
Embryonic stem (ES) cells are cells derived from the inner cell mass of the blastocyst, an early-stage embryo. ES cells are distinguished from other cells due to their pluripotency, which is the ability to differentiate into any different type of cell... Read full blog post.
An Unlikely Pairing: The SOX2 Antibody and Breast Cancer
SOX2 encodes a member of the SOX (SRY-related HMG-box) family of transcription factors, which play a vital role in embryonic development. SOX2 antibody research has identified Sox2 as a key transcription factor in pluripotent stem cells. We at Novus B... Read full blog post.
Fluorescence Activated Cell Sorting Antibody Techniques
Recently, we at Novus Biologicals added several embryonic stem cell marker products to our antibody catalog, validated for use in fluorescent activated cell sorting (FACS) assays. They included Cripto1, PODXL, SSEA, OCT4, Nanog, SOX2, TRA-1, TERT and... Read full blog post.