Nanog Antibody 0.1 ml Embryonic Stem Cell Marker

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Western Blot: Nanog Antibody [NB100-588] - Detection of Mouse Nanog by Western Blot and Immunoprecipitation.
Flow Cytometry: Nanog Antibody [NB100-588] - FACS analysis of Nanog expression in primary mouse hepatic stellate cell cultured for 7 days. Image from verified customer review.
Western Blot: Nanog Antibody [NB100-588] - Detection of Mouse Nanog on HeLa whole cell lysate using NB100-588. Nanog was also IPed using affinity purified rabbit anti-Nanog antibodies NB100-587 and another Nanog.
Flow Cytometry: Nanog Antibody [NB100-588] - staining of NTERA-2  cells using NB100-588 at a 1:50 dilution detected using Dylight-488 conjugated goat anti-rabbit secondary antibody.
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Ordering Information: NB100-588

  • Catalog Number
    NB100-588
  • Displayed Format
    Unconjugated
  • Size(s)
    0.1 ml
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Nanog Antibody Summary

Species Human, Mouse
Tested Applications WB, FLOW, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Gene
NANOG
Purity
Immunogen affinity purified
Specificity
NB100-588 is specific for the Nanog protein.
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Nanog Antibody Details

Isotype
IgG
Marker
Embryonic Stem Cell Marker
Immunogen
A synthetic peptide which maps to a region between residues 250 and the C-terminus (residue 305) of mouse Nanog homeobox using the numbering given in TrEMBL entry Q80Z64 (GeneID 71950).

Species Reactivity

Based on 100% sequence identity, this antibody is predicted to react with Southeastern Asian house mouse, Western Mediterranean mouse and Japanese house mouse.
Reviewed Species Read 2 rated 4
using NB100-588 in the following species:

Publications Read Publications using NB100-588 in the following species:


Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Flow Cytometry 1:150
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
Reviewed Applications
Read 2 Review rated 4
using
NB100-588 in the following applications:

Publications
Read Publications using
NB100-588 in the following applications:

Contact Information

Product PDFs

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Reviews

Average Rating: 4
Recent Reviews
FLOW Tested In Mouse
Reviewed On: 04/29/2014
Rated: 4

Nanog NB100-588
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Publications

Nanog Antibody (NB100-588) has been mentioned in at least 6 publications.
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Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8
Unit Size
0.1 ml
Concentration
1.0 mg/ml
Preservative
0.09% Sodium Azide
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Gene Symbol NANOG
Entrez
Uniprot

Alternate Names for Nanog Antibody

  • FLJ12581
  • FLJ40451
  • hNanog
  • homeobox protein NANOG
  • Homeobox transcription factor Nanog
  • homeobox transcription factor Nanog-delta 48
  • Nanog homeobox
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Related Products by Gene

Background

Nanog is one of the molecular markers suitable for recognizing the undifferentiated state of stem cells in the mouse and human. It is a divergent homeodomain protein that directs propagation of undifferentiated ES cells. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Nanog-deficient ES cells lose pluripotency and differentiate into extraembryonic endoderm lineage.

Reviews for Nanog Antibody (NB100-588) (2) 42

Read what people are saying who have used Nanog Antibody (NB100-588).

We have 2 reviews tested in 2 species: Human, Mouse.
We have 2 reviews tested in 2: Flow Cytometry, Western Blot.
Average Rating: 4
(Based on 2 reviews)

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Images Ratings Applications Species Reviewed By Date Details
Flow Cytometry Nanog NB100-588
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Very Good
FLOW Mouse Guanhua Xie 04/29/2014
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Summary

ApplicationFlow Cytometry
SpeciesMouse
Western Blot Nanog NB100-588
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Very Good
WB Human Erin Kohler 06/08/2011
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Summary

ApplicationWestern Blot
Sample TestedHUVEC, MCF7, JURKAT, Sample Amount: 2-30 ug
SpeciesHuman
Pub Med ID21119109

Blocking

Blocking DetailsBlocking Buffer: 5% milk in TBS, Blocking Time: 1 hour, Blocking Temp: 25 degrees Celsius

Primary Anitbody

Dilution RatioDilution Ratio: 8 ug/ml, Incubation Dilution Buffer: 3% BSA in TBS, Incubation Time: overnight/2 hours, Incubation Temp: 4/25C

Details

Detection NotesDetection Method: Chemiluminescence, Exposure Time: 3 minutes, Specific Bands: 35 kDa

Publications for Nanog Antibody (NB100-588) (6)

Read what people are writing who have used Nanog Antibody (NB100-588).

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: WB, ICC/IF.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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FAQs for Nanog (2)

  1. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
    • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
  2. I was wondering what does Format 7C mean as far as anitbodies go?
    • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)

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Western Blot: c-Myc Antibody (9E10) [NB600-302] - Analysis of c-myc in Jurkat cell lysates using NB600-302.Immunocytochemistry/Immunofluorescence: c-Myc Antibody (9E10) [NB600-302] - HeLa cells stained NB600-302 (Green) detected with DyLight Fluor 488 conjugated anti-mouse IgG secondary antibody.  Nuclei are counterstained with Hoechst 33258 (Blue).Simple Western: c-Myc Antibody (9E10) [NB600-302] - Simple Western lane view shows a specific band for c-Myc in 0.5 mg/ml of Jurkat lysate.  This experiment as performed under reducing conditions using the 12-230 kDa separation system.

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Mouse Monoclonal
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Bioinformatics Tool for Nanog

Discover related pathways, diseases and genes to Nanog. Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Bioinformatics Tool

Research Areas for Nanog

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Blogs on Nanog

Check out the latest blog posts on Nanog.
Nanog is a Master Controller of ES cell Pluripotency
Nanog, a homeodomain (HD) transcription factor, plays a critical role in the maintenance of embryonic stem (ES) cell self-renewal. Transcription regulator involved in inner cell mass and ES cell proliferation and self-renewal. Imposes pluripotency on ...  Read full blog post.

Sox2 and Oct4: Roles in Embryonic Stem Cell Pluripotency
Embryonic stem (ES) cells are cells derived from the inner cell mass of the blastocyst, an early-stage embryo. ES cells are distinguished from other cells due to their pluripotency, which is the ability to differentiate into any different type of cell...  Read full blog post.

An Unlikely Pairing: The SOX2 Antibody and Breast Cancer
SOX2 encodes a member of the SOX (SRY-related HMG-box) family of transcription factors, which play a vital role in embryonic development. SOX2 antibody research has identified Sox2 as a key transcription factor in pluripotent stem cells. We at Novus B...  Read full blog post.

Fluorescence Activated Cell Sorting Antibody Techniques
Recently, we at Novus Biologicals added several embryonic stem cell marker products to our antibody catalog, validated for use in fluorescent activated cell sorting (FACS) assays. They included Cripto1, PODXL, SSEA, OCT4, Nanog, SOX2, TRA-1, TERT and ...  Read full blog post.