Nanog Antibody 0.1 ml Embryonic Stem Cell Marker

Click image to view larger
Western Blot: Nanog Antibody [NB100-588] - Detection of Mouse Nanog by Western Blot and Immunoprecipitation.
Flow Cytometry: Nanog Antibody [NB100-588] - FACS analysis of Nanog expression in primary mouse hepatic stellate cell cultured for 7 days. Image from verified customer review.
Western Blot: Nanog Antibody [NB100-588] - Detection of Mouse Nanog on HeLa whole cell lysate using NB100-588. Nanog was also IPed using affinity purified rabbit anti-Nanog antibodies NB100-587 and another Nanog.
Flow Cytometry: Nanog Antibody [NB100-588] - staining of NTERA-2  cells using NB100-588 at a 1:50 dilution detected using Dylight-488 conjugated goat anti-rabbit secondary antibody.
Download Datasheet
See All Related

Ordering Information: NB100-588

  • Catalog Number
    NB100-588
  • Displayed Format
    Unconjugated
  • Size(s)
    0.1 ml
  • Availability
  • Price
    Shipping
  

Nanog Antibody Summary

Species Human, Mouse
Tested Applications WB, FLOW, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Gene
NANOG
Purity
Immunogen affinity purified
Specificity
NB100-588 is specific for the Nanog protein.
Guarantee Plus
All of our products are backed by our 100% guarantee to work for stated and predicted species and applications.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Nanog Antibody Details

Isotype
IgG
Marker
Embryonic Stem Cell Marker
Immunogen
A synthetic peptide which maps to a region between residues 250 and the C-terminus (residue 305) of mouse Nanog homeobox using the numbering given in TrEMBL entry Q80Z64 (GeneID 71950).

Species Reactivity

Based on 100% sequence identity, this antibody is predicted to react with Southeastern Asian house mouse, Western Mediterranean mouse and Japanese house mouse.
Reviewed Species Read 2 rated 4
using NB100-588 in the following species:

Publications Read Publications using NB100-588 in the following species:


Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Flow Cytometry 1:150
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
Reviewed Applications
Read 2 Review rated 4
using
NB100-588 in the following applications:

Publications
Read Publications using
NB100-588 in the following applications:

Contact Information

Product PDFs

Frequently Purchased Together

Reviews

Average Rating: 4
Recent Reviews
FLOW Tested In Mouse
Reviewed On: 04/29/2014
Rated: 4

Nanog NB100-588
Image 1 of 2
Earn rewards points by submitting your review for Nanog Antibody (NB100-588)

Publications

Nanog Antibody (NB100-588) has been mentioned in at least 6 publications.
Earn rewards if you have published using Nanog Antibody (NB100-588).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8
Unit Size
0.1 ml
Concentration
1.0 mg/ml
Preservative
0.09% Sodium Azide
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Gene Symbol NANOG
Entrez
Uniprot

Alternate Names for Nanog Antibody

  • FLJ12581
  • FLJ40451
  • hNanog
  • homeobox protein NANOG
  • Homeobox transcription factor Nanog
  • homeobox transcription factor Nanog-delta 48
  • Nanog homeobox
Show More

Related Products by Gene

Background

Nanog is one of the molecular markers suitable for recognizing the undifferentiated state of stem cells in the mouse and human. It is a divergent homeodomain protein that directs propagation of undifferentiated ES cells. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Nanog-deficient ES cells lose pluripotency and differentiate into extraembryonic endoderm lineage.

Reviews for Nanog Antibody (NB100-588) (2) 42

Read what people are saying who have used Nanog Antibody (NB100-588).

We have 2 reviews tested in 2 species: Human, Mouse.
We have 2 reviews tested in 2: Flow Cytometry, Western Blot.
Average Rating: 4
(Based on 2 reviews)

Have you used Nanog Antibody (NB100-588)? Submit your review and earn rewards points which can be used for merchandise & discounts.

Sort 2 reviews for Nanog Antibody (NB100-588) by:
Filter by Ratings
Very Good
(2)
All Ratings
Filter by Applications
FLOW
(1)
WB
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
All Species

Images Ratings Applications Species Reviewed By Date Details
Flow Cytometry Nanog NB100-588
Click to Enlarge
Very Good
FLOW Mouse Guanhua Xie 04/29/2014
Show

Summary

ApplicationFlow Cytometry
SpeciesMouse
Western Blot Nanog NB100-588
Click to Enlarge
Very Good
WB Human Erin Kohler 06/08/2011
Show

Summary

ApplicationWestern Blot
Sample TestedHUVEC, MCF7, JURKAT, Sample Amount: 2-30 ug
SpeciesHuman
Pub Med ID21119109

Blocking

Blocking DetailsBlocking Buffer: 5% milk in TBS, Blocking Time: 1 hour, Blocking Temp: 25 degrees Celsius

Primary Anitbody

Dilution RatioDilution Ratio: 8 ug/ml, Incubation Dilution Buffer: 3% BSA in TBS, Incubation Time: overnight/2 hours, Incubation Temp: 4/25C

Details

Detection NotesDetection Method: Chemiluminescence, Exposure Time: 3 minutes, Specific Bands: 35 kDa

Publications for Nanog Antibody (NB100-588) (6)

Read what people are writing who have used Nanog Antibody (NB100-588).

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: WB, ICC/IF.

Have you published using Nanog Antibody (NB100-588)? Submit your review and earn rewards points which can be used for merchandise & discounts.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

Ask a Scientist

Submit your question on below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.

FAQs for Nanog (2)

  1. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
    • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
  2. I was wondering what does Format 7C mean as far as anitbodies go?
    • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)

Customers Who Viewed This Item Also Viewed...

NB100-2379
Western Blot: OCT4 Antibody [NB100-2379] - Detection of OCT4 in mouse brain lysate.Immunocytochemistry/Immunofluorescence: OCT4 Antibody [NB100-2379] - OCT4 staining in NTERA-2 cells detected with a Dylight 488 labeled secondary antibody.Flow Cytometry: OCT4 Antibody [NB100-2379] - Staining of NTERA-2  cells using NB100-2379 at a 1:50 dilution detected using Dylight-488 conjugated goat anti-rabbit secondary antibody.

Rabbit Polyclonal
Species Human, Mouse, Rat...MORE
Applications WB, FLOW, ICC/IF

4 Publications
NB110-37235
Western Blot: SOX2 Antibody [NB110-37235] - Detection of SOX2 in mouse brain lysate using NB110-37235 (0.5ug/ml).Immunocytochemistry/Immunofluorescence: SOX2 Antibody [NB110-37235] - ICC/IF staining of SOX2 in NTERA2 cells with Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained with Dylight 550 (Red) and DAPI (Blue), respectively.Immunohistochemistry-Paraffin: SOX2 Antibody [NB110-37235] - Staining of human uterus, endometrial glands.

Rabbit Polyclonal
Species Human, Mouse, Sheep
Applications WB, FLOW, ICC/IF...MORE

     1 Review

5 Publications
NB600-302
Western Blot: c-Myc Antibody (9E10) [NB600-302] - Analysis of c-myc in Jurkat cell lysates using NB600-302.Immunocytochemistry/Immunofluorescence: c-Myc Antibody (9E10) [NB600-302] - HeLa cells stained NB600-302 (Green) detected with DyLight Fluor 488 conjugated anti-mouse IgG secondary antibody.  Nuclei are counterstained with Hoechst 33258 (Blue).

Mouse Monoclonal
Species Human, Mouse, Drosophila
Applications WB, ChIP, ELISA...MORE

     3 Reviews

19 Publications
NBP2-27406
Western Blot: LIF Antibody (39N7D10) [NBP2-27406] - WB validation of LIF antibody (clone 39N7D10) on (A) full-length recombinant Lif protein, (B) mouse spleen lysate and © human spleen lysate. 3 ug/ml concentration of primary antibody, Goat anti-rat Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this assay.Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - IHC analysis of formalin-fixed paraffin-embedded tissue section of mouse brain using 5 ug/ml concentration of LIF antibody (clone 39N7D10).Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal human prostate using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Cell surface/membrane- cytoplasmic immunepositivity  of LIF was observed specifically in the epithelial cells of prostate alveolar glands whereas the surrounding fibromuscular stroma cells did not develop any staining.

Rat Monoclonal
Species Human, Mouse
Applications WB, IHC-P

NBP2-24749
Western Blot: KLF4 Antibody [NBP2-24749] - Lysate from human 293 cells in the 1) absence and 2) presence of immunizing peptide, 3) mouse NIH 3T3 and 4) RAW cells probed with KLF4 antibody at 1 ug/ml (293) and 2 ug/ml (NIH 3T3 and RAW). I goat anti-rabbit Ig HRP secondary and PicoTect ECL substrate solution were used for this test.Immunohistochemistry-Paraffin: KLF4 Antibody [NBP2-24749] - Breast cancer tissues embedded in paraffin. Negative control is no primary antibody. Image from verified customer review.Flow Cytometry: KLF4 Antibody [NBP2-24749] - Intracellular analysis using KLF4 antibody. Balb/c mouse splenocytes were probed using 1 ug of KLF4 antibody (red) and 1 ug of isotype control was used for this test, and an anti-rabbit IgG PE conjugated secondary .

Rabbit Polyclonal
Species Human, Mouse, Rat...MORE
Applications WB, FLOW, ICC/IF...MORE

     1 Review

3 Publications
NBP1-69368
Western Blot: SLC22A3 Antibody [NBP1-69368] - This Anti-SLC22A3 antibody was used in Western Blot of HepG2 tissue lysate at a concentration of 0.5ug/ml.

Rabbit Polyclonal
Species Human, Bovine, Porcine
Applications WB, IHC, IHC-P

AF3598

Sheep Polyclonal
Species Human
Applications WB, ICC/IF

NBP1-49537
Western Blot: Lin28 Antibody [NBP1-49537] - Analysis of LIN28 in NTERA-2 cell lysateImmunocytochemistry/Immunofluorescence: Lin28 Antibody [NBP1-49537] - Analysis of LIN28 in NTERA-2 cells using NBP1-49537. Nuclei (Blue) are counterstained using Hoechst 33258.Immunohistochemistry: Lin28 Antibody [NBP1-49537] - Immunohistochemical analysis of LIN28 in E17.5 mouse embryo

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ICC/IF, IHC...MORE

NB300-266
Western Blot: Nestin Antibody (10C2) [NB300-266] - Detection of human Nestin using NB300-266.  Lane 1: 5 ug total rat brain protein, lane 2: 5 ug human CNS progenitor cell protein.Immunocytochemistry/Immunofluorescence: Nestin Antibody (10C2) [NB300-266] - Visualization of Nestin in PC-3 cells using NB300-266. Courtesy of Dolnick Lab, Roswell Park Cancer Institute.Flow Cytometry: Nestin Antibody (10C2) [NB300-266] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Nestin antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.

Mouse Monoclonal
Species Human, Mouse (Negative), Rabbit (Negative)...MORE
Applications WB, FLOW, ICC/IF...MORE

     1 Review

11 Publications
NBP2-24463
Western Blot: STAT3 [p Tyr705] Antibody [NBP2-24463] - Analysis of STAT3 (Tyr705) in recombinant fusion protein containing 1) a phosphorylated tyrosine at position 705 and 2) an unphosphorylated tyrosine at position 705, using STAT3 phospho antibody at 0.1 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Rabbit Polyclonal
Species Human, Mouse, Canine...MORE
Applications WB

     1 Review

NB100-1831
Western Blot: SSEA1 Antibody (MC-480) [NB100-1831] - Western blot analysis of Ntera2 cell lysate (A), HUVEC cell lysate (B), Jurkat cell lysate ©, human stomach tissue (D), human brain tissue (E), MEF cell lysate (F), and mouse brain tissue (G) using SSEA 1 antibody (NB100-1831) at 2 ug/ml.Immunocytochemistry/Immunofluorescence: SSEA1 Antibody (MC-480) [NB100-1831] - SSEA1 antibody (0.1ug/ml) was tested in Ntera2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.Flow Cytometry: SSEA1 Antibody (MC-480) [NB100-1831] - Staining of NTERA-2 cells using NB100-1831 at a 1:50 dilution detected using Dylight-488 conjugated goat anti-mouse IgM secondary antibody.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, FLOW, ICC/IF...MORE

4 Publications
NBP1-69686
Western Blot: steroid sulfatase Antibody [NBP1-69686] - This Anti-STS antibody was used in Western Blot of Placenta tissue lysate at a concentration of 1ug/ml.Immunohistochemistry-Paraffin: Steroid sulfatase Antibody [NBP1-69686] - Immunolocalization of Steroid Sulfatase in normal human endometrium  in proliferative phase of the menstrual cycle using NBP1-69686 at a concentration of 4ug/mlImmunohistochemistry-Paraffin: Steroid sulfatase Antibody [NBP1-69686] - Immunolocalization of Steroid Sulfatase in human endometrial tissue sample with endometrial cancer type I using NBP1-69686 at a concentration of 4ug/ml

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

H00008842-G01


Species Human

NBP1-47386
Western Blot: CD44 Antibody (8E2F3) [NBP1-47386] - Western blot analysis using anti-CD44 mAb against Hela (1) and HUVE-12 (2) cell lysates.Immunocytochemistry/Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - Confocal analysis of paraffin-embedded human lung cancer tissues using anti-CD44 mAb (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.Immunohistochemistry-Paraffin: CD44 Antibody (8E2F3) [NBP1-47386] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissues, showing membrane localization with DAB staining using anti-CD44 mAb.

Mouse Monoclonal
Species Human, Mouse, Rabbit (Negative)
Applications WB, ELISA, FLOW...MORE

     1 Review

4 Publications
NBP1-84862
Western Blot: REXO1 Antibody [NBP1-84862] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10<br/>Lane 2: Human cell line RT-4<br/>Lane 3: Human cell line U-251MG sp<br/>Lane 4: Human plasma (IgG/HSA depleted)Immunocytochemistry/Immunofluorescence: REXO1 Antibody [NBP1-84862] - Staining of human cell line U-251 MG shows positivity in nucleus but not nucleoli.Immunohistochemistry-Paraffin: REXO1 Antibody [NBP1-84862] - Staining of human pancreas shows strong granular cytoplasmic positivity in exocrine glandular cells.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC...MORE

NB600-1536
Immunohistochemistry: FGF-2 Antibody [NB600-1536] - [NB600-1356] - Immunoperoxidase staining (PAP) of U87MG glioma cell suspension implanted into nude rat brain (Han rnu/rnu) using Rabbit Anti-Fibroblast Growth Factor-Basic (NB600-1536). High bFGF expression is exclusively shown within tumor endothelial cells. The tissue sample was paraffin-embedded and 4% Tris-buffered formalin-fixed without any enzymatic or antigen-demasking pre-processing. From Dr. A.-C. Stan, Institute of Neuropathology, Medical School Hannover, Hannover, Germany. Ref: Stan, A.C., et al., J. Neurosurg., 82, 1044 (1995).Immunohistochemistry: FGF-2 Antibody [NB600-1536] - Immunoperoxidase staining (PAP) of a fibrillary differentiated astrocytoma (WHO grade I, SAMS grade 1) using Rabbit Anti-Fibroblast Growth Factor-Basic. High bFGF expression is shown within tumor cells as well as within endothelial cells .Immunohistochemistry: FGF-2 Antibody [NB600-1536] - Immunoperoxidase staining (PAP) of a gemistocytic differentiated area of an anaplastic astrocytoma (WHO grade III, SAMS grade 3) using Rabbit Anti-Fibroblast Growth Factor-Basic. High bFGF expression is shown within tumor cells as well as within the endothelial cells.

Rabbit Polyclonal
Species Human, Rat, Bovine...MORE
Applications WB, ELISA, IHC...MORE

2 Publications
NB100-524
Western Blot: NOD2 Antibody (2D9) [NB100-524] - Detection of endogenous NOD2 in 50ug of HT29 cell lysate using NB 100-524. ECL detection in 15 minutes.Immunocytochemistry/Immunofluorescence: NOD2 Antibody (2D9) [NB100-524] - analysis of PFA fixed mouse sciatic nerve section using anti-NOD2 antibody. Image from verified customer review. Western Blot: NOD2 Antibody (2D9) [NB100-524] - Detection of NOD2 in 20ug of NOD2 transfected 293T cell lysate using NB 100-524. ECL detection in 10 seconds.

Mouse Monoclonal
Species Human, Mouse
Applications WB, ICC/IF, IHC...MORE

     1 Review

12 Publications
NBP2-31143
Western Blot: CTLA4 Antibody (37B3D8.2F2) [NBP2-31143] - WB analysis of CTLA-4 protein on (A) mouse Kidney lysate (B) human Kidney lysate and on © partial recombinant protein using CTLA-4 monoclonal antibody at a concentration of 2 ug/ml.

Mouse Monoclonal
Species Human, Mouse
Applications WB

Bioinformatics Tool for Nanog

Discover related pathways, diseases and genes to Nanog. Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Bioinformatics Tool

Research Areas for Nanog

Find related products by research area.

Blogs on Nanog

Check out the latest blog posts on Nanog.
Nanog is a Master Controller of ES cell Pluripotency
Nanog, a homeodomain (HD) transcription factor, plays a critical role in the maintenance of embryonic stem (ES) cell self-renewal. Transcription regulator involved in inner cell mass and ES cell proliferation and self-renewal. Imposes pluripotency on ...  Read full blog post.

Sox2 and Oct4: Roles in Embryonic Stem Cell Pluripotency
Embryonic stem (ES) cells are cells derived from the inner cell mass of the blastocyst, an early-stage embryo. ES cells are distinguished from other cells due to their pluripotency, which is the ability to differentiate into any different type of cell...  Read full blog post.

An Unlikely Pairing: The SOX2 Antibody and Breast Cancer
SOX2 encodes a member of the SOX (SRY-related HMG-box) family of transcription factors, which play a vital role in embryonic development. SOX2 antibody research has identified Sox2 as a key transcription factor in pluripotent stem cells. We at Novus B...  Read full blog post.

Fluorescence Activated Cell Sorting Antibody Techniques
Recently, we at Novus Biologicals added several embryonic stem cell marker products to our antibody catalog, validated for use in fluorescent activated cell sorting (FACS) assays. They included Cripto1, PODXL, SSEA, OCT4, Nanog, SOX2, TRA-1, TERT and ...  Read full blog post.