IRS2 [phospho Ser731] Antibody

Species: Hu Applications: WB, IP, ELISA Clonality: Polyclonal	Gene: IRS2 Purity: Immunogen affinity purified Host: Rabbit
Specificity: This is specific for human IRS 2 phosphorylated at the position of Serine 731. The antibodies were evaluated for specificity with a dot blot assay using synthetically prepared IRS peptides. It only recognizes the phosphorylated serine 731, and does not cross-react with other phosphorylated sites. Publications: Antibody has not yet been mentioned in a publication.
See All IRS2 Products See All IRS2 Related Products: Peptides and Proteins Primary Antibodies
*Note not all species have been tested for reactivity. Only those species listed have been tested. We cannot make any guarantees about additional reactivities or cross reactivities beyond those that have been tested. This antibody may or may not react with other species.

Modification:	phospho Ser731
Isotype:	IgG
Immunogen:	Synthetic peptide (YKASpSPAESS) corresponding to amino acids 727-736 of human IRS 2 at the phosphorylated site of Serine 731. This synthetic peptide also correlates to amino acids 129-138 of mouse IRS 2 at the phosphorylation sites of Serine 133 and to amino acids 229-238 of rat IRS 2 at the serinephosphorylation site 233.
Localization:	Cytoplasmic

Entrez:	8660 (Human)
Related Genes:	IRS1, INSR, AKT1, PIK3CG, IARS, IGF1, INS, PTK2B, IL4, SLC2A4, MAPK1, MAPK3, ECM1, PPP1R13B, ARHGEF7, SHC1, JAK2, PIK3CA, LEP, IGF1R, GH, STAT6, GRB2, GCK, TNF, IRS4, FOXO1, JAK1, MTOR, SREBF1, EPHB1, EPHB2, SGSM3, PDX1, SOCS3, CDKN1B, IL13, SRC, GNRHR, SLC2A2

The insulin receptor mediates insulin action through the phosphorylation of substrate proteins. The major substrates of the insulin receptor kinase are the insulin receptor substrate (IRS) family of proteins, IRS 1, IRS 2, IRS 3, IRS 4. IRS 2, a cytoplasmic signaling molecule that mediates effects of insulin, Insulin-like growthfactor 1 and other cytokines by acting as a molecular adaptor between diverse receptor kinases and downstream effectors. IRS proteins contain more than 70 potential Ser / Thr phosphorylation sites for kinases like PKA (cAMP dependent protein kinase), PKC, and MAPK. The phosphorylation of Ser / Thr residues of IRS proteins has adual function, serving either for a positive or negative modulation of insulin signal transduction.

Reactivity:	Cross-reacts with Human. Not yet tested in other species.

Uses:	Immunoprecipitation, Western Blotting and ELISA This antibody detects a band of approximately 200 kDa in cell lysate derived from mouse serum-treated fibroblasts, which corresponds to the predicted molecular weight of phospho IRS 2. Samples were boiled prior to subject 7.5% SDS-polyacrylamide gel electrophoresis.
Dilutions:	ELISA 0.1 - 1.0 ug/ml, immunoprecipitation 3.0 - 5.0 ug, Western Blot 0.5 - 2.0 ug/ml
Concentrations:	0.5 mg/ml

Buffer:	PBS
Storage:	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Preservative:	0.02% Sodium Azide
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.