NKX6.1 Antibody 0.025 ml

Click image to view larger
Western Blot: Nkx6.1 Antibody [NBP1-49672] - WB analysis of Nkx6.1 in human skeletal muscle.
Immunocytochemistry/Immunofluorescence: Nkx6.1 Antibody [NBP1-49672] - Nkx6.1 antibody was tested at 1:250 in INS-1 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.
Immunohistochemistry-Paraffin: Nkx6.1 Antibody [NBP1-49672] - Human fetal pancreas stained for Nkx6.1, green, and PDX1, grey. Image from verified customer review.
Immunohistochemistry: Nkx6.1 Antibody [NBP1-49672] - IHC staining of Nkx6.1 in mouse intestine using DAB with hematoxylin counterstain.
Download Datasheet
See All Related

Ordering Information: NBP1-49672

  • Catalog Number
    NBP1-49672
  • Displayed Format
    Unconjugated
  • Other Formats
  • Size(s)
  • Availability
  • Price
    Shipping
  

NKX6.1 Antibody Summary

Species Human, Mouse
Tested Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Gene
NKX6-1
Purity
Immunogen affinity purified
Guarantee Plus
All of our products are backed by our 100% guarantee to work for stated and predicted species and applications.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

NKX6.1 Antibody Details

Immunogen
A genomic peptide made to an internal region of the human Nkx6.1 protein (within residues 50-200). [Swiss-Prot P78426]
Localization
Nucleus

Species Reactivity

Human and mouse.
Reviewed Species Read 1 rated 5
using NBP1-49672 in the following species:

Publications Read Publications using NBP1-49672 in the following species:


Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
Application Notes
This Nkx6.1 antibody is useful for Immunohistochemistry-paraffin embedded sections. In IHC-P, staining was observed in the nucleus of mouse intestines. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Other Available
Formats
Alexa Fluor (R) 405 Labeled NBP1-49672AF405
Alexa Fluor (R) 488 Labeled NBP1-49672AF488
Alexa Fluor (R) 647 Labeled NBP1-49672AF647
Alexa Fluor (R) 700 Labeled NBP1-49672AF700
Biotin Labeled NBP1-49672B
DyLight 405 Labeled NBP1-49672V
DyLight 488 Labeled NBP1-49672G
DyLight 550 Labeled NBP1-49672R
DyLight 650 Labeled NBP1-49672C
FITC Labeled NBP1-49672F
HRP Labeled NBP1-49672H
Reviewed Applications
Read 1 rated 5
using
NBP1-49672 in the following applications:

Publications
Read a Publication using
NBP1-49672 in the following applications:

Contact Information

Product PDFs

Frequently Purchased Together

Reviews

Average Rating: 5
Recent Reviews
IHC-P Tested In Human
Reviewed On: 09/24/2014
Rated: 5

NKX6.1 NBP1-49672
Image 1 of 1
Earn rewards points by submitting your review for NKX6.1 Antibody (NBP1-49672)

Publications

NKX6.1 Antibody (NBP1-49672) has been mentioned in at least 1 publication.
Earn rewards if you have published using NKX6.1 Antibody (NBP1-49672).

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 30% glycerol
Unit Size
0.025 ml
Concentration
0.5 mg/ml
Preservative
0.05% Sodium Azide
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Gene Symbol NKX6-1
Entrez
Uniprot

Alternate Names for NKX6.1 Antibody

  • Homeobox protein NK-6 homolog A
  • NK homeo box, family 6, member A
  • NK homeobox (Drosophila), family 6, A
  • NK homeobox, family 6, A
  • NK6 homeobox 1
  • NK6 transcription factor homolog A
  • NK6 transcription factor related, locus 1
  • NK6 transcription factor related, locus 1 (Drosophila)
  • Nkx6.1
  • NKX6.1
  • NKX6Ahomeobox protein Nkx-6.1
Show More

Related Products by Gene

Background

NKX6.1 (homeobox protein Nkx-6.1) is a transcription factor that binds to specific A/T-rich sequences in promoter regions of several genes and is known for its involvement in transcriptional regulation in islet beta cells via NKX6.1-insulin promoter binding mediated control of insulin gene. Together with NKX2-2 as well as IRX3, NKX6.1 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. It belongs to neuronal progenitor factors class II proteins that are induced by SHH signals and is a member of the homeodomain family of transcription factors responsible for multiple organ systems development. The pancreatic development involves many such homeodomain proteins including Hb9, Pdx1, Pax4, Nkx2.2, and importantly on Nkx6.1 and mice deficient for Nkx6.1 display a dramatic reduction in beta-cell numbers in pancreas with near-normal development of other glandular cell types. Moreover, Nkx6.1 deficient mice have significant defects involving V2 interneuron and motor neuron specification in the ventral spinal cord, thereby emphasizing an important parallel in developmental pathways leading to beta-cells and neurons.

Reviews for NKX6.1 Antibody (NBP1-49672) (1) 51

Read what people are saying who have used NKX6.1 Antibody (NBP1-49672).

We have 1 review tested in 1 species: Human.
We have 1 review tested in 1: Immunohistochemistry-Paraffin.
Average Rating: 5
(Based on 1 reviews)

Have you used NKX6.1 Antibody (NBP1-49672)? Submit your review and earn rewards points which can be used for merchandise & discounts.

Sort 1 reviews for NKX6.1 Antibody (NBP1-49672) by:
Filter by Ratings
Excellent
(1)
All Ratings
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
All Species

Images Ratings Applications Species Reviewed By Date Details
Immunohistochemistry-Paraffin NKX6.1 NBP1-49672
Click to Enlarge
Excellent
IHC-P Human Valentina Villani 09/24/2014
Show

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman fetal pancreas
SpeciesHuman
LotB-1

Blocking

Blocking DetailsBSA 2%

Primary Anitbody

Dilution Ratio1/100 dilution. Incubation for 1 hour RT.

Secondary Antibody

Secondary Concentration1/500

Details

Fixation DetailsThe sample was fixed in neutral formalin and paraffin-embedded. Perform antigen retrieval low pH (citrate buffer).

Publications for NKX6.1 Antibody (NBP1-49672) (1)

Read what people are writing who have used NKX6.1 Antibody (NBP1-49672).

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 applications: ICC/IF.

Have you published using NKX6.1 Antibody (NBP1-49672)? Submit your review and earn rewards points which can be used for merchandise & discounts.
Filter By Application
ICC/IF
(1)
All Applications
Filter By Species
Mouse
(1)
All Species

Product General Protocols

View specific protocols for NKX6.1 Antibody (NBP1-49672): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

Ask a Scientist

Submit your question on below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.

FAQs for NKX6.1 (2)

  1. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
    • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
  2. I was wondering what does Format 7C mean as far as anitbodies go?
    • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)

Customers Who Viewed This Item Also Viewed...

NB100-74491
Western Blot: PDX1 Antibody [NB100-74491] - Figure 1 shows a Western blot of pdx-1 in human pancreatic cancer cells MIA-1, PANC-1 and CAPAN-1 using NB100-74491. Figure 2 shows immunofluorescence staining of pdx-1 in mouse islet cells using NB100-74491 and counterstaining (merge) with insulin. The NB100-74491 antibody recognizes both cytoplasmic and nuclear proteins. Formalin-fixed tissues can cause high background, so counterstaining with another antibody which is mainly expressed in the cytoplasm (i.e. insulin) is recommended.Immunocytochemistry/Immunofluorescence: PDX-1/IPF1 Antibody [NB100-74491] - analysis of PDX-1 in mouse pancreas using an anti-PDX-1 antibody. Mouse pancreas with islet (PDX-1 expressing region) is marked with white outline. Image from verified customer review. Immunohistochemistry-Paraffin: PDX1 Antibody [NB100-74491] - Immunohistochemistry was performed on normal biopsies of deparaffinized human tonsil tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0)  buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a Rabbit Polyclonal Antibody recognizing PDX-1 or without primary antibody (negative control) overnight at 4C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor.  Tissues were counterstained with hematoxylin and prepped for mounting.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ICC/IF, IHC...MORE

     2 Reviews

NBP2-15195
Immunohistochemistry-Paraffin: Insulin Antibody (2D11-H5) [NBP2-15195] - Analysisof Insulin in formalin-fixed paraffin embedded human pancreas using Insulin antibody (1:500), peroxidase-conjugate and DAB chromogen. Note the cytoplasmic staining of cells.

Mouse Monoclonal
Species Human, Bovine, Porcine
Applications WB, ELISA, FLOW...MORE

4 Publications
AF3444
Western Blot: Neurogenin-3 Antibody [AF3444] - Lysates of human placenta and prostate tissue. PVDF membrane was probed with 1 ug/mL of Sheep Anti-Human Neurogenin-3 Antigen Affinity-purified Polyclonal Antibody (R&D catalog # AF3444) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (R&D catalog # HAF016). A specific band was detected for Neurogenin-3 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions.

Sheep Polyclonal
Species Human
Applications WB

AF6014
Western Blot: PDHX Antibody [AF6014] - Lysates of 293T human embryonic kidney cell line, mouse heart tissue, and rat heart tissue. PVDF Membrane was probed with 1 ug/mL of Goat Anti-Human/Mouse/Rat PDHX Polyclonal Antibody (R&D catalog # AF6014) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (R&D catalog # HAF109). A specific band was detected for PDHX at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions.

Goat Polyclonal
Species Human, Mouse, Rat
Applications WB

MAB8167

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF

NB300-750
Western Blot: PAX6 Antibody [NB300-750] - Western blot analysis of extracts from HeLa, U251 and mouse brain using PAX6 antibody (NB300-750, 1:100).Immunocytochemistry/Immunofluorescence: PAX6 Antibody [NB300-750] - Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a PAX6 polyclonal antibody at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin were stained with Hoechst 33342 dye.Western Blot: PAX6 Antibody [NB300-750] - Ovine retinal extract

Rabbit Polyclonal
Species Human, Mouse, Rat...MORE
Applications WB, ICC/IF, IHC...MORE

     1 Review

1 Publication
NBP2-22139
Western Blot: Sonic Hedgehog Antibody [NBP2-22139] - WB detection of Sonic Hedgehog in A. human stomach, B. human fetal liver membrane, C. PC12 and D. Cos7Immunocytochemistry/Immunofluorescence: Sonic Hedgehog Antibody [NBP2-22139] - Sonic Hedgehog antibody was tested in SH-SY5Y cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).Immunohistochemistry: Sonic Hedgehog Antibody [NBP2-22139] - IHC analysis of Sonic Hedgehog in mouse kidney.

Rabbit Polyclonal
Species Human, Mouse, Rat...MORE
Applications WB, ICC/IF, IHC...MORE

NBP2-22218
Western Blot: Glucose Transporter GLUT2 Antibody [NBP2-22218] - WB analysis of Glucose Transporter GLUT2 in human pancreas.Immunocytochemistry/Immunofluorescence: Glucose Transporter GLUT2 Antibody [NBP2-22218] - Glucose Transporter GLUT2 antibody was tested in HepG2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).Immunohistochemistry: Glucose Transporter GLUT2 Antibody [NBP2-22218] - IHC staining of Glucose Transporter GLUT2 in mouse liver.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ICC/IF, IHC...MORE

1 Publication
H00004760-M01
Western Blot: NeuroD1 Antibody (3H8) [H00004760-M01] - Analysis of NEUROD1 expression in transfected 293T cell line by NEUROD1 monoclonal antibody (M01), clone 3H8. Lane 1: NEUROD1 transfected lysate (39.89 KDa). Lane 2: Non-transfected lysate.Immunocytochemistry/Immunofluorescence: NeuroD1 Antibody (3H8) [H00004760-M01] - Immunofluorescence of monoclonal antibody to NEUROD1 on HeLa cell . [antibody concentration 10 ug/ml]Immunohistochemistry-Paraffin: NeuroD1 Antibody (3H8) [H00004760-M01] - Immunoperoxidase of monoclonal antibody to NEUROD1 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. [antibody concentration 3ug/ml]

Mouse Monoclonal
Species Human
Applications WB, ELISA, ICC/IF...MORE

NB100-91782
Western Blot: Glucagon Antibody [NB100-91782] - Glucagon antibody (N87) [NB100-91782] - in extracts from COS7 cells.Immunohistochemistry-Paraffin: Glucagon Antibody [NB100-91782] - Human breast carcinoma tissue.Western Blot: Glucagon Antibody [NB100-91782] - Lane1:LO2 cell lysate.  Lane2:Mouse kidney tissue lysate Lane3:H9C2 cell lysate.

Rabbit Polyclonal
Species Human, Mouse, Rat...MORE
Applications WB, ICC/IF, IHC...MORE

1 Publication
NBP1-95731
Western Blot: Islet 1 Antibody (EP4182) [NBP1-95731] (A) K562, (B) HeLa, © Jurkat, and (D) SH-SY5Y cell lysates.Immunocytochemistry/Immunofluorescence: Islet 1 Antibody (EP4182) [NBP1-95731] - On tissue sections from chicken hindbrain.Immunocytochemistry/Immunofluorescence: Islet 1 Antibody (EP4182) [NBP1-95731] - U-87 MG cells.

Rabbit Monoclonal
Species Human, Mouse, Rat...MORE
Applications WB, ICC/IF, IP

NBP2-05030
Western Blot: nkx6.2 Overexpression Lysate (Adult Normal) [NBP2-05030] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for nkx6.2.


Applications WB

AF2614

Goat Polyclonal
Species Human
Applications WB, ICC/IF

NBP1-47778
Western Blot: GCK Antibody (3E3) [NBP1-47778] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GCK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GCK.Immunocytochemistry/Immunofluorescence: GCK Antibody (3E3) [NBP1-47778] Staining of COS7 cells transiently transfected by pCMV6-ENTRY GCK.Immunohistochemistry-Paraffin: GCK Antibody (3E3) [NBP1-47778] - Human prostate tissue.

Mouse Monoclonal
Species Human
Applications WB, ICC/IF, IHC...MORE

MAB70291

Mouse Monoclonal
Species Human
Applications WB

NB100-91966
Western Blot: Somatostatin Antibody [NB100-91966] - Lane1:HepG2 cell lysate. Lane2:Mouse lung tissue lysate.Lane3:NIH-3T3 cell lysate.Immunohistochemistry: Somatostatin Antibody [NB100-91966] - Immunohistochemistry (IHC) analyzes of  Somatostatin (V22) antibody in paraffin-embedded  human colon carcinoma tissue.Western Blot: Somatostatin Antibody [NB100-91966] - Western blot (WB) analysis of Somatostatin (V22)  antibody in extracts from A549 cells

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC...MORE

1 Publication
H00003170-M12
Western Blot: FOXA2 Antibody (6C12) [H00003170-M12] - FOXA2 monoclonal antibody (M12), clone 6C12 Western Blot analysis of FOXA2 expression in K-562 ( Cat # L009V1 ).Immunocytochemistry/Immunofluorescence: FOXA2 Antibody (6C12) [H00003170-M12] - Immunofluorescence of to FOXA2 ( Cat# H00003170-M12 ) on HeLa cell .Immunohistochemistry-Paraffin: FOXA2 Antibody (6C12) [H00003170-M12] - Immunoperoxidase of monoclonal antibody to FOXA2 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 0.5 ug/ml]

Mouse Monoclonal
Species Human
Applications WB, ELISA, ICC/IF...MORE

3 Publications
NBP1-28667
Western Blot: OLIG2 Antibody [NBP1-28667] - Neonatal rat brain lysate showing specific immunolabeling of  the ~ 32k Olig2 protein.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC...MORE

     1 Review

1 Publication
NB600-713
Immunohistochemistry-Paraffin: Amylin Antibody (R10/99) [NB600-713] - Human pancreas with Mouse anti Human Amylin Peptide, showing labeling of Islet of Langerhans.

Mouse Monoclonal
Species Human
Applications ICC/IF, IHC, IHC-Fr...MORE

6 Publications
NB100-1793
Immunohistochemistry-Paraffin: Pancreatic Polypeptide Antibody [NB100-1793] - Adult macaque pancrease paraffin sections following standard protocols.  Image from a confirmed customer review.Immunohistochemistry-Paraffin: Pancreatic Polypeptide Antibody [NB100-1793] - IHC analysis of Pancreatic Polypeptide staining in paraffin embedded Human Pancreas using this antibody (3ug/ml). Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.Immunohistochemistry-Frozen: Pancreatic Polypeptide Antibody [NB100-1793] - IHC analysis of Pancreatic Polypeptide in mouse adult pancreas tissue. Image courtesy of anonymous customer product review.

Goat Polyclonal
Species Human, Mouse, Primate
Applications WB, ICC/IF, IHC...MORE

     2 Reviews

7 Publications