NKX6.1 Antibody Summary
||WB, Simple Western, ICC/IF, IHC, IHC-P
Immunogen affinity purified
All of our products are backed by our 100% guarantee to work for stated and predicted species and applications.
Test in a species/application not listed above to receive a full credit towards a future purchase.
NKX6.1 Antibody Details
A genomic peptide made to an internal region of the human Nkx6.1 protein (within residues 50-200). [Swiss-Prot P78426]
Human and mouse.
Read 1 rated 5 using NBP1-49672 in the following species:
||Read Publications using NBP1-49672 in the following species:
- Western Blot 1:100-1:2000
- Simple Western 1:100
- Immunohistochemistry 1:50-1:100
- Immunohistochemistry-Paraffin 1:50-1:100
This Nkx6.1 antibody is useful for Immunohistochemistry-paraffin embedded sections. In IHC-P, staining was observed in the nucleus of mouse intestines. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Frequently Purchased Together
IHC-P Tested In Human
Image 1 of 1
Earn rewards points by submitting your review for NKX6.1 Antibody (NBP1-49672)
NKX6.1 Antibody (NBP1-49672) has been mentioned in at least 2 publications
Earn rewards if you have published using NKX6.1 Antibody (NBP1-49672).
Manufactured by Genomic Antibody Technology™. GAT FAQs
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS, 30% glycerol
0.05% Sodium Azide
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
Alternate Names for NKX6.1 Antibody
- Homeobox protein NK-6 homolog A
- NK homeo box, family 6, member A
- NK homeobox (Drosophila), family 6, A
- NK homeobox, family 6, A
- NK6 homeobox 1
Related Products by Gene
NKX6.1 (homeobox protein Nkx-6.1) is a transcription factor that binds to specific A/T-rich sequences in promoter regions of several genes and is known for its involvement in transcriptional regulation in islet beta cells via NKX6.1-insulin promoter binding mediated control of insulin gene. Together with NKX2-2 as well as IRX3, NKX6.1 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. It belongs to neuronal progenitor factors class II proteins that are induced by SHH signals and is a member of the homeodomain family of transcription factors responsible for multiple organ systems development. The pancreatic development involves many such homeodomain proteins including Hb9, Pdx1, Pax4, Nkx2.2, and importantly on Nkx6.1 and mice deficient for Nkx6.1 display a dramatic reduction in beta-cell numbers in pancreas with near-normal development of other glandular cell types. Moreover, Nkx6.1 deficient mice have significant defects involving V2 interneuron and motor neuron specification in the ventral spinal cord, thereby emphasizing an important parallel in developmental pathways leading to beta-cells and neurons.
Reviews for NKX6.1 Antibody (NBP1-49672) (1)
Read what people are saying who have used NKX6.1 Antibody (NBP1-49672).
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1: Immunohistochemistry-Paraffin.
Average Rating: 5
(Based on 1 reviews)
Have you used NKX6.1 Antibody (NBP1-49672)? Submit your review and earn rewards points which can be used for merchandise & discounts.
Sort 1 reviews for NKX6.1 Antibody (NBP1-49672) by:
Filter by Ratings
Filter by Applications
Filter by Species
Click to Enlarge
|Sample Tested||Human fetal pancreas|
|Dilution Ratio||1/100 dilution. Incubation for 1 hour RT.|
|Fixation Details||The sample was fixed in neutral formalin and paraffin-embedded. Perform antigen retrieval low pH (citrate buffer).|
Publications for NKX6.1 Antibody (NBP1-49672) (2)
Read what people are writing who have used NKX6.1 Antibody (NBP1-49672).
We have publications tested in 1 confirmed species: Mouse.
We have publications tested in 1 applications: ICC/IF.
Have you published using NKX6.1 Antibody (NBP1-49672)? Submit your review and earn rewards points which can be used for merchandise & discounts.
Filter By Application
Filter By Species
|Theofilopoulos S, Wang Y, Kitambi SS et al. Brain endogenous liver X receptor ligands selectively promote midbrain neurogenesis Nat Chem Biol 2012 Dec 23 [PMID: 23292650] (ICC/IF, Mouse)
|Theofilopoulos S, Griffiths WJ, Crick PJ et al. Cholestenoic acids regulate motor neuron survival via liver X receptors. J. Clin. Invest. 2014 Nov 03 [PMID: 25271621] (ICC/IF, Mouse)
NKX6.1 antibody used for ICC-IF on primary midbrain cultures (mouse E11.5) treated or not with 3beta-7alpha-diHCA , 3 betaH,7O-CA, 3 beta,7 beta-diHCA or 3beta-HCA - 4% PFA fixation, 1 hour bocking with 5% normal goat serum/PBS, primary incubated for ON in PBSpH 7.4 containing 0.3% Triton X-100 and 1% BSA (Figure 4 B).
Product General Protocols
View specific protocols for NKX6.1 Antibody (NBP1-49672):
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
Ask a Scientist
Submit your question on below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us
FAQs for NKX6.1 (2)
- In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
- For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
- I was wondering what does Format 7C mean as far as anitbodies go?
- 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)
Bioinformatics Tool for NKX6.1
Discover related pathways, diseases and genes to NKX6.1. Need help? Read the Bioinformatics Tool Guide
for instructions on using this tool.
Diseases for NKX6.1
Discover more about diseases related to NKX6.1.
Pathways for NKX6.1
View related products by pathway.
Research Areas for NKX6.1
Find related products by research area.
PTMs for NKX6.1
Learn more about PTMs related to NKX6.1.