NKX6.1 Antibody 0.025 ml

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Western Blot: Nkx6.1 Antibody [NBP1-49672] - WB analysis of Nkx6.1 in human skeletal muscle.
Immunocytochemistry/Immunofluorescence: Nkx6.1 Antibody [NBP1-49672] - Nkx6.1 antibody was tested at 1:250 in INS-1 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.
Immunohistochemistry-Paraffin: Nkx6.1 Antibody [NBP1-49672] - Human fetal pancreas stained for Nkx6.1, green, and PDX1, grey. Image from verified customer review.
Immunohistochemistry: Nkx6.1 Antibody [NBP1-49672] - IHC staining of Nkx6.1 in mouse intestine using DAB with hematoxylin counterstain.
Simple Western: NKX6.1 Antibody [NBP1-49672] - Simple Western lane view shows a specific band for NKX6.1 in 0.5 mg/ml of BTC-6 lysate.  This experiment was performed under reducing conditions using the 12-230 kDa separation system.
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Ordering Information: NBP1-49672

  • Catalog Number
    NBP1-49672
  • Displayed Format
    Unconjugated
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NKX6.1 Antibody Summary

Species Human, Mouse
Tested Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Gene
NKX6-1
Purity
Immunogen affinity purified
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NKX6.1 Antibody Details

Immunogen
A genomic peptide made to an internal region of the human Nkx6.1 protein (within residues 50-200). [Swiss-Prot P78426]
Localization
Nucleus

Species Reactivity

Human and mouse.
Reviewed Species Read 1 rated 5
using NBP1-49672 in the following species:

Publications Read Publications using NBP1-49672 in the following species:


Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Simple Western 1:100
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
Application Notes
This Nkx6.1 antibody is useful for Immunohistochemistry-paraffin embedded sections. In IHC-P, staining was observed in the nucleus of mouse intestines. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Other Available
Formats
Alexa Fluor (R) 405 Labeled NBP1-49672AF405
Alexa Fluor (R) 488 Labeled NBP1-49672AF488
Alexa Fluor (R) 647 Labeled NBP1-49672AF647
Alexa Fluor (R) 700 Labeled NBP1-49672AF700
Biotin Labeled NBP1-49672B
DyLight 405 Labeled NBP1-49672V
DyLight 488 Labeled NBP1-49672G
DyLight 550 Labeled NBP1-49672R
DyLight 650 Labeled NBP1-49672C
FITC Labeled NBP1-49672F
HRP Labeled NBP1-49672H
Reviewed Applications
Read 1 rated 5
using
NBP1-49672 in the following applications:

Publications
Read Publications using
NBP1-49672 in the following applications:

Contact Information

Product PDFs

Reviews

Average Rating: 5
Recent Reviews
IHC-P Tested In Human
Reviewed On: 09/24/2014
Rated: 5

NKX6.1 NBP1-49672
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Publications

NKX6.1 Antibody (NBP1-49672) has been mentioned in at least 2 publications.
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Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 30% glycerol
Unit Size
0.025 ml
Concentration
0.5 mg/ml
Preservative
0.05% Sodium Azide
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Gene Symbol NKX6-1
Entrez
Uniprot

Alternate Names for NKX6.1 Antibody

  • Homeobox protein NK-6 homolog A
  • NK homeo box, family 6, member A
  • NK homeobox (Drosophila), family 6, A
  • NK homeobox, family 6, A
  • NK6 homeobox 1
  • NK6 transcription factor homolog A
  • NK6 transcription factor related, locus 1
  • NK6 transcription factor related, locus 1 (Drosophila)
  • Nkx6.1
  • NKX6.1
  • NKX6Ahomeobox protein Nkx-6.1
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Related Products by Gene

Background

NKX6.1 (homeobox protein Nkx-6.1) is a transcription factor that binds to specific A/T-rich sequences in promoter regions of several genes and is known for its involvement in transcriptional regulation in islet beta cells via NKX6.1-insulin promoter binding mediated control of insulin gene. Together with NKX2-2 as well as IRX3, NKX6.1 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. It belongs to neuronal progenitor factors class II proteins that are induced by SHH signals and is a member of the homeodomain family of transcription factors responsible for multiple organ systems development. The pancreatic development involves many such homeodomain proteins including Hb9, Pdx1, Pax4, Nkx2.2, and importantly on Nkx6.1 and mice deficient for Nkx6.1 display a dramatic reduction in beta-cell numbers in pancreas with near-normal development of other glandular cell types. Moreover, Nkx6.1 deficient mice have significant defects involving V2 interneuron and motor neuron specification in the ventral spinal cord, thereby emphasizing an important parallel in developmental pathways leading to beta-cells and neurons.

Reviews for NKX6.1 Antibody (NBP1-49672) (1) 51

Read what people are saying who have used NKX6.1 Antibody (NBP1-49672).

We have 1 review tested in 1 species: Human.
We have 1 review tested in 1: Immunohistochemistry-Paraffin.
Average Rating: 5
(Based on 1 reviews)

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Images Ratings Applications Species Reviewed By Date Details
Immunohistochemistry-Paraffin NKX6.1 NBP1-49672
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Excellent
IHC-P Human 09/24/2014
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman fetal pancreas
SpeciesHuman
LotB-1

Blocking

Blocking DetailsBSA 2%

Primary Anitbody

Dilution Ratio1/100 dilution. Incubation for 1 hour RT.

Secondary Antibody

Secondary Concentration1/500

Details

Fixation DetailsThe sample was fixed in neutral formalin and paraffin-embedded. Perform antigen retrieval low pH (citrate buffer).

Publications for NKX6.1 Antibody (NBP1-49672) (2)

Read what people are writing who have used NKX6.1 Antibody (NBP1-49672).

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 applications: ICC/IF.

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Product General Protocols

View specific protocols for NKX6.1 Antibody (NBP1-49672): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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FAQs for NKX6.1 (3)

  1. Has this antibody been tested on cryo sections for immunohistochemistry?
    • Unfortunately the antibody has never been tested with any frozen section (so called IHC-Fr). This antibody had been tested, in lab, for using in WB, ICC/IF, and IHC-P as these were already posted on the web. Our company use "IHC" just for a faster indexing/searching or browsing the webpages. In theory, when an antibody product can be used in IHC-P, usually it can be used in IHC-Fr with the same dilutions; but not the way around. Therefore you could consider to include NBP1-49672 for using in your ongoing IHC-Fr experiments.
  2. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
    • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
  3. I was wondering what does Format 7C mean as far as anitbodies go?
    • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)