Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
The immunogen for this antibody was a sequence from 114a.a. to 285a.a. from NKCC1(a 1212a.a. long protein)
Localization:
Membrane; multi-pass membrane protein (Probable). Microsome.
Species Reactivity:
This antibody is reactive against Human, chimpanzee, dog, mouse.
Applications:
Uses:
Western Blot: E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:2000. Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development.
Dilutions:
Western Blot
Unit Size:
0.05 mg
Concentration:
Please see the vial label for concentration.
Packaging:
Storage:
Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles.
Buffer:
Phosphate-Buffered Saline.
Preservative:
No Preservative
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Western Blot: E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. ( anti-SLC12A2
[7] , Vibat C.R., Holl M.J., Kang J.J., Putney L.K., O#039;Donnell M.E. Quantitation of Na(+)-K(+)-2Cl(-) cotransport splice variants in human tissues using kinetic polymerase chain reaction.
[6] , Payne J.A., Xu J.-C., Haas M., Lytle Y.C., Ward D., Forbush B. III Primary structure, functional expression, chromosomal localization of the bumetanide-sensitive Na-K-Cl cotransporter in human colon.
[5] Simard,C.F., Brunet,G.M., Daigle,N.D., Montminy,V., Caron,L. Isenring,P. Self-interacting domains in the C terminus of a cation-Cl- cotransporter described for the first time
[4] Simard,C.F., Daigle,N.D., Bergeron,M.J., Brunet,G.M., Caron,L., Noel,M., Montminy,V. Isenring,P. Characterization of a novel interaction between the secretory Na+-K+-Cl- cotransporter the chaperone hsp90
[3] Liedtke,C.M., Wang,X. Smallwood,N.D., et al. Role for protein phosphatase 2A in the regulation of Calu-3 epithelial Na+-K+-2Cl-, type 1 co-transport function
[2] Dzhala,V.I., Talos,D.M., Sdrulla,D.A., Brumback,A.C., Mathews,G.C., Benke,T.A., Delpire,E., Jensen,F.E. Staley,K.J. NKCC1 transporter facilitates seizures in the developing brain
[1] Panet,R., Eliash,M. Atlan,H., et al. Na+/K+/Cl- cotransporter activates MAP-kinase cascade downstream to protein kinase C, upstream to MEK