RelA/NFkB p65 Antibody

Name: RelA/NFkB p65 Antibody
Brand: Novus Biologicals
SKU: NB100-78423
Price: 349 USD
Availability: Discontinued

(1 Publication)

See All Related

Submit a Review

Images

Product Details

Summary

Product Discontinued

Order Details

Catalog Number

NB100-78423
Availability

Product Discontinued

Can't find what you are looking for? Use our Antibody Concierge Service & we will help you locate your antibody!

Or feel free to contact us for alternative products.

RelA/NFkB p65 Antibody Summary

Immunogen	peptide-KLH, C-terminal
Specificity	NF-kB p65
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	RELA
Purity	Protein A or G purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Applications/Dilutions

Dilutions

Immunocytochemistry/ Immunofluorescence 1:100-1:400
Immunohistochemistry 1:10-1:500
Immunohistochemistry-Paraffin 1:10-1:500
Immunoprecipitation 1:10-1:500
Western Blot 1:500

Application Notes

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 ul per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1:100~1:400. It is recommended that the reagent be titrated for optimal performance for each application.

Theoretical MW

65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Control

RelA/NFkB p65 Overexpression Lysate

Publications

Read Publication using
NB100-78423 in the following applications:

WB
1 publication

Packaging, Storage & Formulations

Storage	Store at 4C. Do not freeze.
Buffer	PBS (pH 7.2) and 0.2% Gelatin
Preservative	0.09% Sodium Azide
Concentration	0.5 mg/ml
Purity	Protein A or G purified

Alternate Names for RelA/NFkB p65 Antibody

MGC131774
nf kb p65
NFkB p65
NF-kB p65
NFKB3
NFKB3v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor ofkappa light polypeptide gene enhancer in B-cells 3 (p65))
Nuclear factor NF-kappa-B p65 subunit
Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3transcription factor p65
p65
p65RelA
rela p65
RelA
v-rel reticuloendotheliosis viral oncogene homolog A (avian)
v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappalight polypeptide gene enhancer in B-cells 3, p65

Background

NF- kappaB/p65 (nuclear factor kappa light chain enhancer in B cells p65) is a member of the Rel/dorsal family. This ubiquitously expressed nuclear protein is one subunit of the NF-kappa B complex consisting of a 65 kD transactivating subunit and a 50 kD DNA binding subunit. Both subunits are derived from larger precursor proteins. NF- kappaB acts as a transcriptional activator and was originally identified as an activator of kappa light chain in B cells. NF- kappaB/p65 is bound (with p50) to I kappaB inhibitor in cytoplasm in inactive form. Phosphorylation of I kappaB releases and NF- kappaB which goes to the nucleus to activate gene expression. NF- kappaB can be activated with LPS, TNF- alpha, phorbol ester, or IL-1. The Poly6226 antibody reacts with the C-terminal region of human, mouse, and rat NF- kappaB p50 and has been shown to be useful for Western blotting.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NFkB p105/p50
Antibody (2J10D7) - B ...

NB100-56583

(1 Review)

(5 Publications)

Species: Hu, Rb, Rt

Applications: Flow, IHC, IHC-P, KO, Simple Western, WB

TNF-alpha
[Unconjugated]

210-TA

(3 Reviews)

(850 Publications)

Species: Hu

Applications: BA

CD40/TNFRSF5
Antibody [Unconjugate

AF632

Western Blot CD40/TNFRSF5 Antibody [Unconjugated]

Simple Western CD40/TNFRSF5 Antibody [Unconjugated]

(8 Publications)

Species: Hu

Applications: AgAct, Simple Western, WB

ARHGEF7 Antibody -
BSA Free

NBP1-88650

Immunohistochemistry-Paraffin ARHGEF7 Antibody - BSA Free

Species: Hu

Applications: IHC, IHC-P, KD, WB

ASCC1 Antibody
(OTI6G5)

NBP2-01345

(1 Review)

Species: Hu, Mu, Rt

Applications: Flow, ICC/IF, IHC, IHC-P, WB

IL-6 [HRP]

M6000B

(716 Publications)

Species: Mu

Applications: ELISA

c-Rel Antibody
[Unconjugated]

AF2699

Western Blot c-Rel Antibody [Unconjugated]

(1 Review)

(4 Publications)

Species: Mu

Applications: Simple Western, WB

c-jun [p Ser63]
Antibody (SY0297)

NBP2-67471

Immunohistochemistry-Paraffin c-jun [p Ser63] Antibody (SY0297)

(1 Review)

(1 Publication)

Species: Hu, Mu, Rt

Applications: ICC/IF, IHC, IHC-P, WB

IkB-alpha Antibody
(6A920) - BSA Free

NB100-56507

Flow Cytometry IkB-alpha Antibody (6A920) - BSA Free

(42 Publications)

Species: Hu, Mu, Rt

Applications: CyTOF-ready, Flow, ICC/IF, IHC, IHC-P, IP, KO, Simple Western, WB

iNOS Antibody -
BSA Free

NB300-605

(3 Reviews)

(143 Publications)

Species: Hu, Mu, Po, Rt

Applications: Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, WB

ERK2 Antibody
[Unconjugated]

AF1230

Immunohistochemistry ERK2 Antibody [Unconjugated]

Knockout Validated ERK2 Antibody [Unconjugated]

(4 Publications)

Species: Hu, Mu, Rt

Applications: IHC, KO, WB

COX-2 Antibody

NB100-689

(1 Review)

(36 Publications)

Species: Hu, Rt

Applications: IHC, IHC-Fr, IHC-P, Simple Western, WB

c-Fos Antibody
(2H2) - BSA Free

NBP2-50037

Immunocytochemistry/ Immunofluorescence c-Fos Antibody (2H2) - BSA Free

(1 Review)

(25 Publications)

Species: Hu, Mu, Rt

Applications: ICC/IF, IHC, KO, WB

Neurofascin
Antibody

AF3235

Immunohistochemistry Neurofascin Antibody

(1 Review)

(38 Publications)

Species: Hu, Mu, Rt

Applications: IHC, WB

NFkB2/NFkB p100
Antibody - BSA Free

NBP1-87760

(1 Publication)

Species: Hu

Applications: ICC/IF, IHC, IHC-P, KD, Simple Western, WB

ISYNA1 Antibody -
BSA Free

NBP2-47415

Immunohistochemistry-Paraffin ISYNA1 Antibody - BSA Free

Species: Hu

Applications: ICC/IF, IHC, IHC-P, WB

AKT [p Ser473]
Antibody [Unconjugate ...

AF887

Simple Western AKT [p Ser473] Antibody [Unconjugated] - Pan Specific

Intracellular Staining by Flow Cytometry AKT [p Ser473] Antibody [Unconjugated] - Pan Specific

(52 Publications)

Species: Hu, Mu, Rt

Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB

ERK1 [p Thr202, p
Tyr204] [Biotin]

DYC1825-2

(12 Publications)

Species: Hu, Mu, Rt

Applications: ELISA

JNK1 Antibody
(228601) [Unconjugate

MAB17761

Immunocytochemistry JNK1 Antibody (228601) [Unconjugated]

(1 Review)

(3 Publications)

Species: Hu, Mu, Rt

Applications: ICC, WB

RelA/NFkB p65
Antibody

NB100-78423

Species: Hu, Mu, Rt

Applications: WB, ICC/IF, IHC, IP

Publications for RelA/NFkB p65 Antibody (NB100-78423)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.

Filter By Application

(1)

All Applications

Filter By Species

Mouse

(1)

All Species

Showing Publication 1 - 1 of 1.

Publication using NB100-78423	Applications	Species
de la Pisa IG, Cebrian C, Ortega JE et al. Cytokine pathway disruption in a mouse model of schizophrenia induced by Munc18-1a overexpression in the brain. J Neuroinflammation 2014-07-29 [PMID: 25069615] (WB, Mouse) Details: CD11b antibody used for WB application at 1:1000 dilution in mouse model of schizophrenia induced by Munc18-1a overexpression/OE in the brain (see full text for sample preparation details). Figures 3a and 4a shows the WB of microglia /macrophage marker CD11bas single band of 160 kDa in cerebral cortex and striatum tissues of Munc18-OE and wild-type mice.	WB	Mouse

Reviews for RelA/NFkB p65 Antibody (NB100-78423) (0)

There are no reviews for RelA/NFkB p65 Antibody (NB100-78423). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.

Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

ICC/IF Video Protocol

FAQs for RelA/NFkB p65 Antibody (NB100-78423) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

RelA/NFkB p65 Overexpression Lysate

Secondary Antibodies

Isotype Controls

Additional RelA/NFkB p65 Products

Research Areas for RelA/NFkB p65 Antibody (NB100-78423)

Find related products by research area.

Blogs on RelA/NFkB p65.

Check out the latest blog posts on RelA/NFkB p65.

Killing two birds with one stone: Treating inflammation and cancer by inhibiting prolyl-4-hydroxylase-1
By Jamshed Arslan Pharm.D. The cell’s oxygen-sensing machinery comprises prolyl-4-hydroxylases (P4Hs 1-3, PHDs 1-3, or EGLN 1-3) and their canonical target hypoxia-inducible factors (HIFs). When oxygen levels ... Read full blog post.

The effect of antioxidants and the NFkB p65 pathway in inflammation
NFkB is a transcription factor that plays a role in the expression of genes involved in immune response, inflammation, metastasis, cell survival and more. RelA (p65) is one member of the NFkB mammalian family, alongside other subunits. NFkB subunit... Read full blog post.

NFkB3-p65: Say that three times fast!
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes such as inflammation, immunity, differentiation, cell growth, tumor genesis and apoptosis. Unlike the majority of transcription factors that reside in the nucleus... Read full blog post.

Contact Information

Product PDFs

SDS

PDF Datasheet

Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

Volume

Mass

Concentration

Review this Product

Be the first to review our RelA/NFkB p65 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol	RELA
Entrez	5970(Human) 19697(Mouse) 309165(Rat)
Uniprot	Q04206()

Popular Products	Company Information	Support	Stay Connected
Antibodies Secondary Antibodies Isotype Controls Proteins and Peptides ELISA Kits Sample Size New Products	About Us Careers Novus Guarantee Contact Us Our Team	Technical Support FAQs Protocols

Visit our other sites

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

RelA/NFkB p65 Antibody

Species Glossary

Images

Product Details

Order Details

RelA/NFkB p65 Antibody Summary

Applications/Dilutions

Packaging, Storage & Formulations

Alternate Names for RelA/NFkB p65 Antibody

Background

Limitations

Customers Who Viewed This Item Also Viewed...

Publications for RelA/NFkB p65 Antibody (NB100-78423)(1)

Reviews for RelA/NFkB p65 Antibody (NB100-78423) (0)

Product General Protocols

Video Protocols

FAQs for RelA/NFkB p65 Antibody (NB100-78423) (0)

Control Lysate(s)

Secondary Antibodies

Isotype Controls

Additional RelA/NFkB p65 Products

Research Areas for RelA/NFkB p65 Antibody (NB100-78423)

Blogs on RelA/NFkB p65.

Contact Information

Product PDFs

Calculators

Review this Product

Bioinformatics