NFkB p65 Antibody (NB110-57267)
NFkB p65 Antibody (E379)
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anti-MGC131774 antibody, anti-v-rel reticuloendotheliosis viral oncogene homolog A antibody, anti-V Rel Avian Reticuloendotheliosis Viral Oncogene Homolog A antibody, anti-Transcription Factor p65 antibody, anti-RELA antibody, anti-Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B Cells antibody, anti-Nuclear Factor NF Kappa B p65 Subunit antibody, anti-NFKB3 antibody, anti-NFKB 3 antibody |
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NFkB p65 Antibody (E379) Summary: | | Gene: | RELA |
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| Purity: | Tissue culture supernatant |
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| Host: | Rabbit |
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| | | | Publications: | Antibody has been mentioned in at least 1 Publication. |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional NFkB p65 Products NFkB p65 Antibody (E379) Details: | Clone: | E379 |
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| Isotype: | IgG |
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| Immunogen: | A synthetic peptide corresponding to residues near the C-terminus of human nuclear factor NF-kappa-B p65 subunit was used as immunogen. |
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| Uses: | This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation, Flow Cytometry. In Western blot this antibody detects a band at approximately 65kDa. |
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| Dilutions: | Western Blot 1:1000-10000, Immunohistochemistry-Paraffin 1:250-500, Immunocytochemistry 1:250-500, Immunoprecipitation 1:200, Flow Cytometry 1:100 |
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| TMW: | 65 kDa |
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| Unit Size: | 0.1 ml |
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| Concentration: | This product is unpurified. Concentration is not relevant. |
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| Positive Controls: | 1 Positive Controls |
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Packaging: | Storage: | Store at -20 °C. Avoid freeze-thaw cycles. |
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| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
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| Preservative: | 0.01% Sodium Azide |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: Nuclear factor B (NF-B) encompasses an important family of inducible transcriptional activators that regulate a wide variety of cellular and viral genes (1). The family members include p50, p52, p65 (RelA), c-Rel, and RelB. (2). The p65 subunit, similar to two others in the B family, RelB and c-Rel, contains two transactivation domains in the C-terminal region of the protein (3). Association with inhibitory proteins of the IêB family, retains NF-êB in the cytoplasm. Degradation of IB proteins exposes the nuclear localization sequence (NLS), leading to nuclear translocation and subsequent binding of NF-B to DNA (4). In addition to the nuclear translocation and DNA binding, NF-B's transcriptional activity is also regulated by coactivators and corepressors in the nucleus. Interaction of p65 with coactivators; CREB-binding protein and p300, increases its transcriptional activity (5).
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Images (3) Reviews (0) Related Products (91) Exploring NFkB p65 Working with NFkB p65
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Jump to: Positive Controls, Antibody Pairs, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies
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Related ProductsPositive ControlsAntibody Pairs (8)Lysates (1)Peptides and ProteinsPrimary Antibodies (40)| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| NFkB p65 Antibody | NBP1-96139 | Hu, Mk, Mu | ICC, IF, IHC-P, WB | (3) | | | | | NFkB p65 Antibody | NBP1-96138 | Hu, Mk, Mu | ICC, IF, IHC-P | (2) | | | | | NFkB p65 Antibody (27F9.G4) | NBP1-77815 | Hu | ELISA, IF, IHC-P, WB | (4) | | | | | NFkB p65 Antibody | NB100-2176 | Hu, Mu, Rt | IHC-P, IP, WB | (3) | (1) | | | | NFkB p65 Antibody (Poly6226) | NB100-78423 | Hu, Mu, Rt | IF, IHC-Fr, IHC-P, IP, WB | (2) | (2) | | | | ... and 35 more. See All » |
RNAi (1)Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Rabbit IgG Antibody [HRP] | NB730-H | Rb | ELISA, ICC, IHC-P, WB | | (5) | | | | Rabbit IgG Antibody [FITC] | NB730-F | Rb | ICC, IHC-P | | (1) | | | | Rabbit IgG Antibody [Biotin] | NB730-B | Rb | ELISA, ICC, IHC-P, WB | | | | | | Rabbit IgG, H/L Chains Antibody [DyLight 488] | NBP1-72944 | Rb | FLISA, IF, WB | (3) | | | | | Rabbit IgG Antibody [Alkaline Phosphatase] | NB730-AP | Rb | ELISA, ICC, IHC-P, WB | | (1) | | | | See All » |
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![Immunohistochemistry: NF-kB p65 Antibody (E379) [NB110-57267] - Immunohistochemical analysis of paraffin-embedded human Breast carcinoma using anti-NF-kB p65 (NB110-57267).](http://images.novusbio.com/images/p_57267_2.jpg "Immunohistochemistry: NF-kB p65 Antibody (E379) [NB110-57267] - Immunohistochemical analysis of paraffin-embedded human Breast carcinoma using anti-NF-kB p65 (NB110-57267).")
![Western Blot: NF-kB p65 Antibody (E379) [NB110-57267] - Western blot analysis on Hela cell lysate using anti- NF-kB p65 (NB110-57267). dilution 1:100,000.](http://images.novusbio.com/images/p_57267_1.jpg "Western Blot: NF-kB p65 Antibody (E379) [NB110-57267] - Western blot analysis on Hela cell lysate using anti- NF-kB p65 (NB110-57267). dilution 1:100,000.")
![Immunofluorescence: NF-kB p65 Antibody (E379) [NB110-57267] - Immunofluorescent staining of HeLa cells using anti anti-NF-kB p65 (NB110-57267).](http://images.novusbio.com/images/p_57267_3.jpg "Immunofluorescence: NF-kB p65 Antibody (E379) [NB110-57267] - Immunofluorescent staining of HeLa cells using anti anti-NF-kB p65 (NB110-57267).")
