- Products
- Research Areas
- Epigenetics
- Amino Acids, Drugs and other small molecules
- Apoptosis
- Autophagy
- Cancer
- Cell Biology
- Cell Cycle and Replication
- Cellular Markers
- Cytoskeleton
- DNA Repair
- Epitope Tags
- GPCR
- Hypoxia
- Immunology
- Lipid and Metabolism
- Loading Controls
- MAP Kinase Signaling
- Membrane Trafficking and Chaperones
- Neuroscience
- Nuclear Receptors, Coactivators and Corepressors
- Phospho-Specific
- Protein Kinase
- Protein Phosphatase
- Protein Turnover
- Signal Transduction
- Stem Cell Markers
- Transcription Factors and Regulators
- Translation Control
- Tyrosine Kinases
- Wnt Signaling Pathway
- modENCODE Antibodies
- Support
- Distributors
- About
Support: 303-730-1950
Worldwide (Choose your country.)
![Western Blot: NFkB p65 Antibody (27F9.G4) [NBP1-77815] - NFKB p65 (Rel A) monoclonal antibody was used to detect ~65 kD band (red arrow) in HeLa whole cell lysate. Lysate was run on 4-20% gradient gel transferred under standard conditions and blocked in 1% BSA-TTBS 30 min RT. Blot was probed with monoclonal anti p65 1:1000 in 1% BSA-TBS-T o/n 4C and detected with HRP conjugated Rb-anti Mouse antibody 1:40,000 in blocking buffer 30 min RT.](http://images.novusbio.com/images/NFkB p65 Antibody (27F9.G4)-Western Blot-NBP1-77815-img0001.jpg "Western Blot: NFkB p65 Antibody (27F9.G4) [NBP1-77815] - NFKB p65 (Rel A) monoclonal antibody was used to detect ~65 kD band (red arrow) in HeLa whole cell lysate. Lysate was run on 4-20% gradient gel transferred under standard conditions and blocked in 1% BSA-TTBS 30 min RT. Blot was probed with monoclonal anti p65 1:1000 in 1% BSA-TBS-T o/n 4C and detected with HRP conjugated Rb-anti Mouse antibody 1:40,000 in blocking buffer 30 min RT.")
![Western Blot: NFkB p65 Antibody (27F9.G4) [NBP1-77815] - 12% Bis-Tris Gels. Blot was incubated with primary antibody at a dilution of 1:500 and detected with HRP conjugated anti mouse antibody at a dilution of 1:10000.](http://images.novusbio.com/images/NFkB p65 Antibody (27F9.G4)-Western Blot-NBP1-77815-img0003.jpg "Western Blot: NFkB p65 Antibody (27F9.G4) [NBP1-77815] - 12% Bis-Tris Gels. Blot was incubated with primary antibody at a dilution of 1:500 and detected with HRP conjugated anti mouse antibody at a dilution of 1:10000.")
![Immunohistochemistry-Paraffin: NFkB p65 Antibody (27F9.G4) [NBP1-77815] - Immunohistochemistry-Paraffin: NFkB p65 Antibody (27F9.G4) [NBP1-77815] Human tissues. showed moderate to strong staining within many tissues, including epithelium of the breast, small intestine, kidney, pancreas, prostate, skin, placenta, and uterus, as well as within neurons and lymphoid tissues such as spleen, thymus, and tonsil. The antibody produced an excellent signal with almost no background staining at a concentration of 2.5 ug/ml. The image displayed shows specific staining in colon carcinoma as the precipitated red signal, with a hematoxylin purple nuclear counterstain.](http://images.novusbio.com/images/NFkB p65 Antibody (27F9.G4)-Immunohistochemistry-Paraffin-NBP1-77815-img0004.jpg "Immunohistochemistry-Paraffin: NFkB p65 Antibody (27F9.G4) [NBP1-77815] - Immunohistochemistry-Paraffin: NFkB p65 Antibody (27F9.G4) [NBP1-77815] Human tissues. showed moderate to strong staining within many tissues, including epithelium of the breast, small intestine, kidney, pancreas, prostate, skin, placenta, and uterus, as well as within neurons and lymphoid tissues such as spleen, thymus, and tonsil. The antibody produced an excellent signal with almost no background staining at a concentration of 2.5 ug/ml. The image displayed shows specific staining in colon carcinoma as the precipitated red signal, with a hematoxylin purple nuclear counterstain.")
![Immunofluorescence: NFkB p65 Antibody (27F9.G4) [NBP1-77815] - confluent on 18 mm2 glass coverslips #1.5. Cells were either unstimulated (A), or stimulated (B) with 50 ng/ml of TNF alpha for 30 min prior fixation. Cells were then fixed in methanol and blocked with 10% normal goat serum (NGS), in PBS, and TritonX 0.2% (Tx) and incubated for 1 hr at RT with primary ab, counterstained with DAPI and washed in PBS/NGS/Tx. Cells were incubated for 1 hr at RT with Atto 425 conjugated anti mouse secondary antibody for STED CW imaging.0](http://images.novusbio.com/images/NFkB p65 Antibody (27F9.G4)-Immunofluorescence-NBP1-77815-img0002.jpg "Immunofluorescence: NFkB p65 Antibody (27F9.G4) [NBP1-77815] - confluent on 18 mm2 glass coverslips #1.5. Cells were either unstimulated (A), or stimulated (B) with 50 ng/ml of TNF alpha for 30 min prior fixation. Cells were then fixed in methanol and blocked with 10% normal goat serum (NGS), in PBS, and TritonX 0.2% (Tx) and incubated for 1 hr at RT with primary ab, counterstained with DAPI and washed in PBS/NGS/Tx. Cells were incubated for 1 hr at RT with Atto 425 conjugated anti mouse secondary antibody for STED CW imaging.0")
