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NEK9 [p Thr210] Antibody

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Product Details

Summary
Product Discontinued
View other related NEK9 Primary Antibodies

Order Details


    • Catalog Number
      NB100-78432
    • Availability
      Product Discontinued

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NEK9 [p Thr210] Antibody Summary

Immunogen
Partial recombinant protein, responding to the middle region of NEK-9
Modification
p Thr210
Specificity
NEK9 [Thr210]
Clonality
Polyclonal
Host
Rabbit
Gene
NEK9
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:500
Application Notes
IHC & IP reported in the literature. Antigen retrieval for IHC of formalin-fixed paraffin-embedded tissue using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Theoretical MW
107 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
NEK9 Overexpression Lysate

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 50% Glycerol
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for NEK9 [p Thr210] Antibody

  • DKFZp434D0935
  • KIAA1995
  • MGC138306
  • MGC16714
  • Nek8
  • Nek8NERCC1
  • NEK9
  • NERCC
  • Nercc1 kinase
  • NERCCEC 2.7.11.1
  • Never in mitosis A-related kinase 9
  • NIMA (never in mitosis gene a)- related kinase 9
  • NimA-related kinase 8
  • NIMA-related kinase Nek8
  • NimA-related protein kinase 9
  • serine/threonine-protein kinase Nek9

Background

NEK9 is a 107 kD member of the serine/threonine-protein kinase family, NIMA subfamily containing a coiled-coil domain, and RCC1 repeats. This cytoplasmic protein forms dimers and is a pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation, Nek9 is activated during mitosis by intramolecular autophosphorylation at Thr210. Nek9 has been shown to interact with Ran GTPase (greater affinity for Ran-GDP over Ran-GTP), NEK6, NEK7, BICD2, histone H3, myelin basic protein, and beta-casein. The Poly6241 antibody recognizes human NEK9 and has been shown to be useful for Western blotting, immunohistochemistry, and immunoprecipitation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NEK9 [p Thr210]
Antibody
NB100-78432
NEK9 [p Thr210] Antibody
Species: Hu
Applications: WB, IHC

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Control Lysate(s)

NEK9 Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NEK9
Entrez
Uniprot