NBS1 Antibody (NB100-143)
NBS1 Antibody
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anti-AT V1 antibody, anti-p95 antibody, anti-Nijmegen breakage syndrome protein 1 antibody, anti-Nijmegen breakage syndrome antibody, anti-Nibrin antibody, anti-NBS1 antibody, anti-NBS Antibody, anti-NBS 1 antibody, anti-NBN antibody, anti-MGC87362 antibody, anti-FLJ10155 antibody, anti-Cell cycle regulatory protein p95 antibody, anti-ATV antibody, anti-AT V2 antibody |
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NBS1 Antibody Summary: | | Gene: | NBN |
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| Purity: | Whole antisera |
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| Host: | Rabbit |
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| | | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional NBS1 Products NBS1 Antibody Details: | Immunogen: | Partial length human NBS1 protein [Swiss-Prot# O60934]. |
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| Localization: | Nuclear |
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| Epitope: | The epitope falls between residues 300-750 |
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| Uses: | Western blot, immunoprecipitation and immunofluorescence. By Western blot, this NBS1 antibody recognizes a band at 95 kDa, representing NBS1. By immunofluorescence, this antibody has been used with methanol-fixed IMR90 primary human fibroblasts. Immunoprecipitation tests have been done with 3-4 X 106 cells. |
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| Dilutions: | Immunocytochemistry 1:50-1:200, Immunofluorescence 1:200, immunoprecipitation 3 ul, Western Blot 1:1000 |
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| Unit Size: | 0.05 ml (also available: 0.025 ml (sample size)) |
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| Concentration: | This product is unpurified. Concentration is not relevant. |
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| Positive Controls: | 2 Positive Controls |
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Packaging: | Storage: | Store at 4 °C. Do not freeze. |
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| Buffer: | Whole antisera |
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| Preservative: | 0.02% Sodium Azide |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: The p95 gene (identical to NBS1 and nibrin) is a member of the hMre11/hRad50 double-strand break complex. This protein complex has been implicated in Nijmegen breakage syndrome, an autosomal recessive disorder marked by increased cancer incidence, cell cycle checkpoint deficits, and ionizing radiation sensitivity, thus revealing a direct molecular link between double-strand break repair and cell cycle checkpoint functions.
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Images (2) Reviews (0) Related Products (44) Exploring NBS1 Working with NBS1
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Jump to: Publications, Images, Protocols/Procedures, Ask a Question
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Working with NBS1
NBS1 Antibody Images (2) Immunocytochemistry: NBS1 Antibody [NB100-143] - ICC staining of Hela cells using NB100-143 anti-NBS1. |  Western Blot: NBS1 antibody [NB100-143] - Detection of human NBS1 using NB 100-143. |
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Publications (78)NBS1 Antibody Product Specific:- Bohgaki T, Bohgaki M, Cardoso R, et al. Genomic Instability, Defective Spermatogenesis, Immunodeficiency, and Cancer in a Mouse Model of the RIDDLE Syndrome. PLoS Genet. 2011 Apr;7.[PMID 21552324]
- Howlett NG, Taniguchi T, Durkin SG, et al. The Fanconi anemia pathway is required for the DNA replication stress response and for the regulation of common fragile site stabilit. Hum Mol Genet. 2005 Mar 1;14(5):693-701. [PMID: 15661754]
more... - So S, Davis AJ, Chen DJ. Autophosphorylation at serine 1981 stabilizes ATM at DNA damage sites. J Cell Biol. 2009 Dec 28;187(7):977-90. [PMID: 20026654]
- Yan T, Seo Y, Kinsella TJ. Differential cellular responses to prolonged LDR-IR in MLH1-proficient and MLH1-deficient colorectal cancer HCT116 cells. Clin Cancer Res. 2009 Nov 15;15(22):6912-20. [PMID: 19861440]
- Mirzoeva, O. K., et al. The Mre11/Rad50/Nbs1 complex interacts with the mismatch repair system and contributes to temozolomide-induced G2 arrest and cytotoxicity. Mol Cancer Ther. 2006 Nov;5(11):2757-66.
- McCabe, N., et al. Deficiency in the repair of DNA damage by homologous recombination and sensitivity to poly(ADP-ribose) polymerase inhibition. Cancer Res. 2006 Aug 15;66(16):8109-15.
- Dote, H., et al. Inhibition of hsp90 compromises the DNA damage response to radiation. Cancer Res. 2006 Sep 15;66(18):9211-20.
- Verdun, R., et al. Functional human telomeres are recognized as DNA damage in G2 of the cell cycle. Mol Cell. 2005 Nov 23;20(4):551-61.
- Gueven, N., et al. Defective p53 response and apoptosis associated with an ataxia-telangiectasia-like phenotype. Cancer Res. 2006 Mar 15;66(6):2907-12.
- Brown, K., et al. The mismatch repair system is required for S-phase checkpoint activation. Nat Genet. 2003 Jan;33(1):80-4. Epub 2002 Nov 25.
- Lukas, C., et al. Distinct spatiotemporal dynamics of mammalian checkpoint regulators induced by DNA damage. Nat Cell Biol. 2003 Mar;5(3):255-60.
- Giannini, G., et al. Human MRE11 is inactivated in mismatch repair-deficient cancers. EMBO Rep. 2002 Mar;3(3):248-54. Epub 2002 Feb 15.
- Huang, Juren, et al. Reconstitution of the mammalian DNA double-strand break end-joining reaction reveals a requirement for an Mre11/Rad50/NBS1-containing fraction. Nucleic Acids Res. 2002 Feb 1;30(3):667-74.
- Stracker, T.H., etal. Adenovirus oncoproteins inactivate the Mre11-Rad50-NBS1 DNA repair complex. Nature. 2002 Jul 18;418(6895):348-52.
- Gatei, M., et al. ATM-dependent phosphorylation of nibrin in response to radiation exposure. Nat Genet. 2000 May;25(1):115-9.
- Wu, X., et al. Independence of R/M/N focus formation and the presence of intact BRCA1. Science. 2000 Jul 7;289(5476):11.
- Schwartz RA, Carson CT, Schuberth C, et al. Adeno-Associated Virus Replication Induces a DNA Damage Response Coordinated by DNA-Dependent Protein Kinase. J Virol 2009;83(12):6269-6278. [pubmed: 19339345]
- Shimada M, Sagae R, Kobayashi J, et al. Inactivation of the Nijmegen Breakage Syndrome Gene Leads to Excess Centrosome Duplication via the ATR/BRCA1 Pathway. 2009;69(5):1768-1775. [pubmed: 19244116]
- Nahas SA, Butch AW, Du L, et al. Rapid Flow Cytometry-Based Structural Maintenance of Chromosomes 1 (SMC1) Phosphorylation Assay for Identification of Ataxia-Telangiectasia Homozygotes Heterozygotes. Clin Chem 2009:clinchem.2008.107128 [pubmed: 19147735].
- Karen KA, Hoey PJ, Young CSH, et al. Temporal regulation of the Mre11-Rad50-Nbs1 complex during adenovirus infection. J Virol. 2009 May;83(9):4565-73. [pubmed: 19244322]
- Quanz M, Berthault N, Roulin C, et al. Small-Molecule Drugs Mimicking DNA Damage: A New Strategy for Sensitizing Tumors to Radiotherapy. 2009;15(4):1308-1316. [pubmed: 19190126]
- Blickwedehl J, Agarwal M, Seong C, et al. Role for proteasome activator PA200 and postglutamyl proteasome activity in genomic stability. Proc Natl Acad Sci U S A. 2008 Oct 21;105(42):16165-70. Epub 2008 Oct 9.
- Ewald B, Sampath D, Plunkett W, et al. ATM and the Mre11-Rad50-Nbs1 complex respond to nucleoside analogue-induced stalled replication forks and contribute to drug resistance. Cancer Res. 2008 Oct 1;68(19):7947-55.
- Lakdawala SS, Schwartz RA, Ferenchak K, et al. Differential requirements of the C terminus of Nbs1 in suppressing adenovirus DNA replication and promoting concatemer formation. J Virol. 2008 Sep;82(17):8362-72. Epub 2008 Jun 18.
- Li L, Monckton EA, et al. A role for DEAD box 1 at DNA double-strand breaks. Mol Cell Biol. 2008 Oct;28(20):6413-25. Epub 2008 Aug 18.
- Schwartz RA, Lakdawala SS, Eshleman HD, et al. Distinct requirements of adenovirus E1b55K protein for degradation of cellular substrates. J Virol. 2008 Sep;82(18):9043-55. Epub 2008 Jul 9.
- Bouska A, Lushnikova T, Plaza S, et al. Mdm2 promotes genetic instability and transformation independent of p53. Mol Cell Biol. 2008 Aug;28(15):4862-74. Epub 2008 Jun 9.
- Spycher C, Miller ES, Townsend K, et al. Constitutive phosphorylation of MDC1 physically links the MRE11-RAD50-NBS1 complex to damaged chromatin. J Cell Biol. 2008 Apr 21;181(2):227-40. Epub 2008 Apr 14.
- Zhao X, Madden-Fuentes RJ, Lou BX, et al. Ataxia telangiectasia-mutated damage-signaling kinase- and proteasome-dependent destruction of Mre11-Rad50-Nbs1 subunits in Simian virus 40-infected primate cells. J Virol. 2008 Jun;82(11):5316-28. Epub 2008 Mar 19.
- Gatz SA, Keimling M, Baumann C, et al. Resveratrol modulates DNA double-strand break repair pathways in an ATM/ATR-p53- and -Nbs1-dependent manner. Carcinogenesis. 2008 Mar;29(3):519-27. Epub 2008 Jan 3.
- Tanaka, T., A. Munshi, et al. Gefitinib radiosensitizes non-small cell lung cancer cells by suppressing cellular DNA repair capacity. Clin Cancer Res. 2008 Feb 15;14(4):1266-73.
- Dery U, A, et al. glycine-arginine domain in control of the human MRE11 DNA repair protein. Mol Cell Biol. 2008 May;28(9):3058-69. Epub 2008 Feb 19.
- Cervelli, T., J. A. Palacios, et al. Processing of recombinant AAV genomes occurs in specific nuclear structures that overlap with foci of DNA-damage-response proteins. J Cell Sci. 2008 Feb 1;121(Pt 3):349-57.
- Compton, S.A., et al. Xrcc3 and Nbs1 are required for the production of extrachromosomal telomeric circles in human alternative lengthening of telomere cells. Cancer Res. 2007 Feb 15;67(4):1513-9.
- Kato, T., N. Sato, et al. Activation of Holliday junction recognizing protein involved in the chromosomal stability and immortality of cancer cells. Cancer Res. 2007 Sep 15;67(18):8544-53. Epub 2007 Sep 6.
- Kruger, L., et al. Cancer incidence in Nijmegen breakage syndrome is modulated by the amount of a variant NBS protein. Carcinogenesis. 2007 Jan;28(1):107-11. Epub 2006 Jul 13.
- Stewart, G.S. et al. RIDDLE immunodeficiency syndrome is linked to defects in 53BP1-mediated DNA damage signaling. Proc Natl Acad Sci U S A. 2007 Oct 23;104(43):16910-5. Epub 2007 Oct 16.
- Schwartz, R. A. et al. The Mre11/Rad50/Nbs1 complex limits adeno-associated virus transduction and replication. J Virol. 2007 Dec;81(23):12936-45. Epub 2007 Sep 26.
- Jacquemont, C. Taniguchi, T. Proteasome function is required for DNA damage response and fanconi anemia pathway activation. Cancer Res. 2007 Aug 1;67(15):7395-405.
- Takemura, H., et al. Defective Mre11-dependent activation of Chk2 by ataxia telangiectasia mutated in colorectal carcinoma cells in response to replication-dependent DNA double strand breaks. J Biol Chem. 2006 Oct 13;281(41):30814-23. Epub 2006 Aug 10.
- Varon, R., et al. Mild Nijmegen breakage syndrome phenotype due to alternative splicing. Hum Mol Genet. 2006 Mar 1;15(5):679-89. Epub 2006 Jan 13.
- Seemanova, E., et al. Nijmegen breakage syndrome (NBS) with neurological abnormalities and without chromosomal instability. J Med Genet. 2006 Mar;43(3):218-24. Epub 2005 Jul 20.
- Myers, J.S. Cortez, D. Rapid activation of ATR by ionizing radiation requires ATM and Mre11. J Biol Chem. 2006 Apr 7;281(14):9346-50. Epub 2006 Jan 23.
- Tseng, S-F., et al. Importin KPNA2 is required for proper nuclear localization and multiple functions of NBS1. J Biol Chem. 2005 Nov 25;280(47):39594-600. Epub 2005 Sep 27.
- Yang, MW., et al. Increased NBS1 expression is a marker of aggressive head and neck cancer and overexpression of NBS1 contributes to transformation. Clin Cancer Res. 2006 Jan 15;12(2):507-15.
- Sheen, M.R., et al. Mre11-Rad50-Nbs1 complex is activated by hypertonicity. Am J Physiol Renal Physiol. 2006 Nov;291(5):F1014-20. Epub 2006 Jun 20.
- Pan, J., et al. Farnesyltransferase inhibitors induce DNA damage via reactive oxygen species in human cancer cells. Cancer Res. 2005 May 1;65(9):3671-81.
- Lilley, C.E., et al. DNA repair proteins affect the lifecycle of herpes simplex virus 1. Proc Natl Acad Sci U S A. 2005 Apr 19;102(16):5844-9. Epub 2005 Apr 11. [Human, Western Blot]
- Alt, J.R., et al. Mdm2 binds to Nbs1 at sites of DNA damage and regulates double strand break repair. J Biol Chem. 2005 May 13;280(19):18771-81. Epub 2005 Feb 25. [Immunofluorescence, Human]
- Laposa, R.R. et al. Recapitulation of the cellular xeroderma pigmentosum-variant phenotypes using short interfering RNA for DNA polymerase H. Cancer Res. 2003 Jul 15;63(14):3909-12. [Western Blot, Human]
- Donahue, S.L. et al. Deficient regulation of DNA double-strand break repair in Fanconi anemia fibroblasts. J Biol Chem. 2003 Aug 8;278(32):29487-95. Epub 2003 May 14. [Western Blot, Human]
- Fan, R., et al. Defective DNA strand break repair after DNA damage in prostate cancer cells: implications for genetic instability and prostate cancer progression. Cancer Res. 2004 Dec 1;64(23):8526-33.
- Donahue, S., et al. A Rad50-dependent pathway of DNA repair is deficient in Fanconi anemia fibroblasts. Nucleic Acids Res. 2004 Jun 15;32(10):3248-57. Print 2004. [Human]
- Kurz, E., et al. Doxorubicin activates ATM-dependent phosphorylation of multiple downstream targets in part through the generation of reactive oxygen species. J Biol Chem. 2004 Dec 17;279(51):53272-81. Epub 2004 Oct 14. [Western Blot, Immunoprecipitation, Immunofluorescence, Human]
- Kitagawa, R., et al. Phosphorylation of SMC1 is a critical downstream event in the ATM-NBS1-BRCA1 pathway. Genes Dev. 2004 Jun 15;18(12):1423-38. Epub 2004 Jun 2. [Western Blot, Immunofluorescence, Mouse/Human]
- Demuth. I., et al. An inducible null mutant murine model of Nijmegen breakage syndrome proves the essential function of NBS1 in chromosomal stability and cell viability. Hum Mol Genet. 2004 Oct 15;13(20):2385-97. Epub 2004 Aug 27. [Mouse]
- Demuth, I., et al. Human SNM1B is required for normal cellular response to both DNA interstrand crosslink-inducing agents and ionizing radiation. Oncogene. 2004 Nov 11;23(53):8611-8.
- Zhang, J., et al. Chk2 phosphorylation of BRCA1 regulates DNA double-strand break repair. Mol Cell Biol. 2004 Jan;24(2):708-18. [Immunofluorescence, Immunoprecipitation, Western Blot, Human]
- Wilkinson, D., et al. Recruitment of cellular recombination and repair proteins to sites of herpes simplex virus type 1 DNA replication is dependent on the composition of viral proteins within prereplicative sites and correlates with the induction of the DNA damage response. J Virol. 2004 May;78(9):4783-96. [Immunofluorescence, Western Blot, Monkey]
- Collis, S., et al. Evasion of early cellular response mechanisms following low level radiation-induced DNA damage. J Biol Chem. 2004 Nov 26;279(48):49624-32. Epub 2004 Sep 17.[Immunofluorescence, Western Blot, Flow Cytometry, Human]
- Lukas, C., et al. Mdc1 couples DNA double-strand break recognition by Nbs1 with its H2AX-dependent chromatin retention. EMBO J. 2004 Jul 7;23(13):2674-83. Epub 2004 Jun 17. [Human]
- Cerosaletti, K., et al. Independent roles for nibrin and Mre11-Rad50 in the activation and function of Atm. J Biol Chem. 2004 Sep 10;279(37):38813-9. Epub 2004 Jul 1. [Immunofluorescence, Western Blot, Human]
- Fabbro, M., et al. BRCA1-BARD1 Complexes Are Required for p53(Ser-15) Phosphorylation a G(1)/S Arrest following Ionizing Radiation-induced DNA Damage. JBC. 279(30): 31251-31258, 2004. [Immunofluorescence, Western Blot, Human] [Immunofluorescence, Western Blot, Human]
- Krause, D. Suppression of Tousled-like kinase activity after DNA damage or replication block requires ATM, NBS1 and Chk1. Oncogene. 2003 Sep 4;22(38):5927-37.
- Cheng, W., et al. Linkage between Werner syndrome protein and the Mre11 complex via Nbs1. J Biol Chem. 2004 May 14;279(20):21169-76. Epub 2004 Mar 16. [Western Blot, Immunofluorescence, Immunoprecipitation, Human]
- Lee, J., et al. Distinct functions of Nijmegen breakage syndrome in ataxia telangiectasia mutated-dependent responses to DNA damage. Mol Cancer Res. 2003 Jul;1(9):674-81. [Immunoprecipitation, Western Blot, Human]
- Xu, X., Stern, DF. NFBD1/MDC1 regulates ionizing radiation-induced focus formation by DNA checkpoint signaling and repair factors. FASEB J. 2003 Oct;17(13):1842-8. [Immunofluorescence, Human]
- Davalos, A., Campisi, J. Bloom syndrome cells undergo p53-dependent apoptosis and delayed assembly of BRCA1 and NBS1 repair complexes at stalled replication forks. J Cell Biol. 2003 Sep 29;162(7):1197-209. [Western Blot, Immunoprecipitation, Immunofluorescence, Human]
- Udayakumar, D., et al. Distinct pathways of nonhomologous end joining that are differentially regulated by DNA-dependent protein kinase-mediated phosphorylation. J Biol Chem. 2003 Oct 24;278(43):41631-5. Epub 2003 Aug 12. [Western Blot, Human]
- Wu, G., et al. NBS1 and TRF1 colocalize at promyelocytic leukemia bodies during late S/G2 phases in immortalized telomerase-negative cells. Implication of NBS1 in alternative lengthening of telomeres. J Biol Chem. 2000 Sep 29;275(39):30618-22. [Immunoprecipitation, Human]
- Beamish, H., et al. Functional link between BLM defective in Bloom's syndrome and the ataxia-telangiectasia-mutated protein, ATM. J Biol Chem. 2002 Aug 23;277 (34):30515-23. Epub 2002 May 28. [Western Blot, Immunoprecipitation, Flow Cytometry, Human]
- Siwicki, J., et al. Telomere maintenance and cell cycle regulation in spontaneously immortalized T-cell lines from Nijmegen breakage syndrome patients. Exp Cell Res. 2003 Jul 1;287(1):178-89. [Western Blot, Immunoprecipitation, Flow Cytometry, Human]
- Cerosaletti, K: Nibrin forkhead-associated domain and breast cancer C-terminal domain are both required for nuclear focus formation and phosphorylation. J Biol Chem. 2003 Jun 13;278(24):21944-51. Epub 2003 Apr 4. [Immunoprecipitation, Western Blot, Immunofluorescence, Human]
- Paull, T., et al. A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage. Curr Biol. 2000 Jul 27-Aug 10;10(15):886-95. [Immunofluorescence, Human]
- Franchitto, A., et al. Bloom's syndrome protein is required for correct relocalization of RAD50/MRE11/NBS1 complex after replication fork arrest. J Cell Biol. 2002 Apr 1;157(1):19-30. Epub 2002 Mar 26. [Western Blot, Immunoprecipitation, Immunofluorescence, Human]
- Wang, et al. BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures. Genes Dev. 2000 Apr 15;14 (8):927-39.[Western Blot, Immunoprecipitation, Immunofluorescence, Human]
- Buscemi, Giacomo., et al. Chk2 activation dependence on Nbs1 after DNA damage. Mol Cell Biol. 2001 Aug;21(15):5214-22. [Western Blot, Immunoprecipitation, Immunofluorescence, Human]
- Goldberg, M., et al. MDC1 is required for the intra-S-phase DNA damage checkpoint. Nature. 2003 Feb 27;421(6926):952-6. [Immunofluorescence, Human]
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Jump to: Positive Controls, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies
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Related ProductsPositive Controls| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| NBS1 Lysate | NBL1-13497 | | WB | (1) | | | | Other Positive Controls- TK6 and HeLa whole cell extract (NB 800-PC1)
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Lysates (1)Peptides and ProteinsPrimary Antibodies (32)| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| NBS1 Antibody | NBP1-06705 | Hu, Mu | IHC-P, WB | (2) | (1) | | | | NBS1 Antibody | NBP1-06609 | Hu, Mu | IHC-P, WB | (2) | | | | | NBS1 Antibody (Y112) | NB110-57272 | Hu, Mu, Rt | ICC, IHC-P, IP, WB | (3) | (1) | | | | NBS1 Antibody | NB100-277 | Hu | IP, WB | (1) | (1) | | | | NBS1 Antibody (1D7) | NB100-221 | Hu | IP, WB | (3) | (2) | | | | ... and 27 more. See All » |
RNAi (2)Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Rabbit IgG Antibody [HRP] | NB730-H | Rb | ELISA, ICC, IHC-P, WB | | (5) | | | | Rabbit IgG Antibody [FITC] | NB730-F | Rb | ICC, IHC-P | | (1) | | | | Rabbit IgG Antibody [Biotin] | NB730-B | Rb | ELISA, ICC, IHC-P, WB | | | | | | Rabbit IgG, H/L Chains Antibody [DyLight 488] | NBP1-72944 | Rb | FLISA, IF, WB | (3) | | | | | Rabbit IgG Antibody [Alkaline Phosphatase] | NB730-AP | Rb | ELISA, ICC, IHC-P, WB | | (1) | | | | See All » |
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