We are looking for an NBS mouse monoclonal antibody that would work for IF. NBS1 Antibody (NB100-221) might be ideal, except we need to use it in mouse cells, and the data sheet says it is reactive with human cells. Could you tell us if it has been tested in mouse cells? If it has not, we would be willing to test it, and send you verifying images. However, if we test it and it does not work in mouse cells, would we be able to receive another antibody in exchange? We would also like to purchase a STAT1 mouse monoclonal antibody that would work for IF. STAT1 Antibody (NBP1-48011). The species reactivity of the antibody indicates that it is reactive with human, canine, and primate cells. Has it been tested in mouse cells?

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I have a couple of thoughts after reading through your needs for this antibody. This antibody is validated for mouse reactivity. I assume you realize that if you want to use a mouse mAb on mouse tissue, you will need to do a mouse on mouse block to prevent non-specific staining with your secondary. If you would like to test an antibody in an unvalidated species or application, please consider our Innovators Reward Program. STAT1, NBP1-48011, has not been validated for mouse reactivity so this would qualify for the Innovators Reward Program.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Immunogen	Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human p95 NBS1 aa 650-750 (C terminal).
Isotype	IgG
Clonality	Monoclonal
Host	Rabbit
Gene	NBN
Purity	Tissue culture supernatant
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Store at -20C. Avoid freeze-thaw cycles.
Buffer	59% PBS (pH 7.2), 0.05% BSA and 40% Glycerol
Preservative	0.01% Sodium Azide
Purity	Tissue culture supernatant

Publications using NB110-57272	Applications	Species
Chen G, Zhang B, Xu H et al. Suppression of Sirt1 sensitizes lung cancer cells to WEE1 inhibitor MK-1775-induced DNA damage and apoptosis Oncogene 2017-09-04 [PMID: 28869605] (WB, Human)	WB	Human
Hartlerode AJ, Morgan MJ, Wu Y et al. Recruitment and activation of the ATM kinase in the absence of DNA-damage sensors. Nat. Struct. Mol. Biol. 2015-08-17 [PMID: 26280532] (WB, Mouse)	WB	Mouse
Mason JM, Das I, Arlt M et al. The SNM1B/APOLLO DNA nuclease functions in resolution of replication stress and maintenance of common fragile site stability. Hum Mol Genet 2013-07-30 [PMID: 23863462] (ICC/IF, Human)	ICC/IF	Human
Bensimon A, Schmidt A, Ziv Y et al. ATM-dependent and -independent dynamics of the nuclear phosphoproteome after DNA damage. Sci Signal 2010-12-01 [PMID: 21139141]
Uhl M, Csernok A, Aydin S et al. Role of SIRT1 in homologous recombination. DNA Repair (Amst) 2010-04-01 [PMID: 20097625] (WB)	WB
Momcilovi? O, Choi S, Varum S et al. Ionizing radiation induces ataxia telangiectasia mutated-dependent checkpoint signaling and G(2) but not G(1) cell cycle arrest in pluripotent human embryonic stem cells. Stem Cells 2009-08-01 [PMID: 19544417]
Smith CJ. Mechanisms in suppressing chromosomal translocations and maintaining genome stability. Clinical Colorectal Cancer. 2014-09-21 (WB, Mouse) Details: NBS1 antibody used for WB in experiments involving Artemis+/+ and ArtemisP70/P70 mouse embryonic fibroblasts	WB	Mouse
Blickwedehl J, Agarwal M, Seong C et al. Role for proteasome activator PA200 postglutamyl proteasome activity in genomic stability. PNAS;105(42):16165-16170. 2008-01-01 [PMID: 18845680]

	Verified Customer 01/07/2015
Application:	WB
Species:	Human

Nbs1 Antibody (Y112)

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Gene Symbol	NBN
Entrez	4683(Human) 27354(Mouse) 85482(Rat)
Uniprot	O60934()