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Abeta 42 Antibody

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-05001
    • Availability
      Product Discontinued

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Abeta 42 Antibody Summary

Immunogen
This polyclonal antibody was raised by immunizing rabbit with a synthetic peptide containing 1-40 amino acids of amyloid.
Specificity
Amyloid beta (37-42)
Clonality
Polyclonal
Host
Rabbit
Gene
APP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Dot Blot
  • Western Blot
Application Notes
The antibody specificity was assayed by dot blot analysis with the synthetic Amyloid (1-40) peptide antigen and Western blot analysis. The antibody titer is more than 10K for Dot blot and 1,000X for Western blot analysis (data not shown). It has not been tested in the other applications. However, for the first testing, we recommend 1/5,000 dilution for ELISA, 1/1,000 dilution for Western blot analysis (WB) of recombinant protein, 1/400 dilution for tissue extracts or cell lysates, 1/100 dilution for immunohistochemistry (IHC) staining on frozen cryosections, 1/50 dilution for IHC staining on paraffin embedded sections.

Reactivity Notes

Chemical

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
No buffer
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Please contact technical support for detailed reconstituion instruction.

Notes

Add diH20 for desired concentration.

Alternate Names for Abeta 42 Antibody

  • Amyloid beta 42
  • Beta-amyloid protein 42

Background

The amyloid beta peptide (A beta) is the main component of amyloid deposits found in the brain of patients afflicted with Alzheimer's disease. Brain to blood transport is believed to be a major determinant of the amount of amyloid beta protein (AbetaP) found in brain. Chronic brain hypoperfusion (CBH) initiates spatial memory loss in aging rats thus mimicking human mild cognitive impairment (MCI) and also increases A beta in the hippocampus. The memory and amyloid changes are preceded by NO upregulation in the hippocampus. These preliminary findings may be important in understanding, at least in part, the molecular mechanisms that precede memory impairment during chronic brain ischemia and as such, the pre-clinical stage leading to Alzheimer's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional Abeta 42 Products

Research Areas for Abeta 42 Antibody (NBP1-05001)

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Blogs on Abeta 42.

Using Amyloid beta peptides in Alzheimer's Disease Immunization
Amyloid beta (AB) peptide has a central role in the neurodegeneration of Alzheimer's disease (AD). Immunization of AD transgenic mice with AB-42 peptide reduces both the spatial memory impairments and AD-like neuropathologic changes.Therapeutic im...  Read full blog post.

Amyloid beta and Methionine Sulfoxide Related to Abeta 42 Antibody and Abeta 40 Antibody
By Eric NeeleyAlzheimer's disease is a devastating neurodegenerative illness characterized by the formation of plaques, tangles, and eventually synaptic loss. Amyloid beta (A beta ) is the processed form of the Amyloid precursor protein (APP), and whose ...  Read full blog post.

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Bioinformatics

Gene Symbol APP