Acetylcholinesterase Lysate

Applications: WB

Gene: ACHE Purity: Protein

Specificity:	Homo sapiens acetylcholinesterase (Yt blood group) (ACHE), transcript variant E4-E5, mRNA.

Immunogen:	The lysate was created in HEK293T cells, using plasmid ID RC211249 and based on accession number NM_015831.

Type: Overexpression Tissue Condition: Normal

Life Stage: Adult Protein State: Native Sub-cellular Fraction: Whole

Entrez:	43 (Human)
Swiss Prot:	NM_015831 (Human)
Related Genes:	CHAT, CHAT, CHAT, BCHE, BCHE, BCHE, CAT, CAT, CAT, SH2D3C, SH2D3C, MIP, GLYAT, TRPV6, GLYAT, NGF, CSF1, SPP1, VIP, VIP, TH, TAC1, TAC1, NGF, G4, NGF, CHRNA2, VIP, NPY, NPY, NPY, AFP, AFP, CALCA, APP, ES1, H2-AB1, PNPLA6, PNPLA6, ACE

Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. [provided by RefSeq]. Transcript Variant: The splice variant E4-E5 is primarily expressed in erythroid tissues, and appears to encode the PI-linked form of acetylcholinesterase. It results from tissue-specific alternative splicing of exon 4 to exon 5. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Uses:	This product is intended for use as a positive control in Western Blot.
Dilutions:	Western Blot
TMW:	60.8 kDa
Concentrations:	1 mg/ml

This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Buffer:	This product is provided in modified RIPA buffer.
Storage:	Store at -20C. Avoid freeze-thaw cycles.
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.