Hela Whole Cell Lysate

Applications: WB	Purity: Protein
Specificity: NB800_PC1 contains HeLa whole cell extract lysate.
See All Hela Whole Cell Products See All Hela Whole Cell Related Products: Lysates

Type: Cell Tissue: Reproductive Tissue Condition: Normal

Life Stage: Adult Sub-cellular Fraction: Whole

NB800-PC1 whole cell lysate is derived from a cell line or tissue using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot analysis using antibodies to key cell signaling components to confirm the presence of both high molecular weight and low molecular weight proteins.

Uses:	Lysates are prepared as positive controls for separation by SDS-PAGE and subsequent Western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). Heat lysate to 95 degrees C for 5 minutes and rapidly cool. If dissociating conditions are desired, add reducing agent prior to heating.
Dilutions:	Western Blot
Concentrations:	2.5 mg/ml

Protein supplied in 1x Laemmli Sample Buffer without 2-mercaptoethanol. 2-Mercaptoethanol should be added to 5% prior to use.

Buffer:	1X SDS-PAGE Sample Buffer [62.5 mM Tris HCl, 2% SDS, 10% Glycerol, 0.005% bromophenol blue, pH 6.8]
Storage:	Store at -80C. Avoid freeze-thaw cycles.
Preservative:	No Preservative
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.