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Summary:
Clonality: Polyclonal
Specificity: Antibody recognizes the HA cleavage site.
Purity: Immunogen affinity purified
Host: Chicken
Details:
Immunogen: Chickens were immunized with HA cleavage site (YPYDVPDYA) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support. Chicken anti- human HA affinity purified antibodies were coupled to peptide immobilized on solid support.
 
Background:
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
Species:
Non-species specific
Reactivity: Epitope Tag. Non-species specific..
*Note not all species have been tested for reactivity. Only those species listed have been tested. We cannot make any guarantees about additional reactivities or cross reactivities beyond those that have been tested. This antibody may or may not react with other species.
Applications:
IP, IF, ICC, WB, ELISA
Uses: Suggested working dilutions: * ELISA Coating 1:100-1:500Primary 1:1000-1:30,000 Western Blots Colorimetric detection 1:1000-1:10,000ECL 1:1000-1:30,000 Cytochemistry 1:100-1:400 *The investigator should determine the optimal working dilution for a specific application. ELISA, Western blot, and immunocytochemistry.
Dilutions: ELISA, Immunocytochemistry, immunofluorescence, immunoprecipitation, Western Blot
Packaging:
Storage: Store at 4C. Do not freeze.
Buffer: Phosphate Buffered Saline, pH 7.2
Preservative: 0.09% Sodium Azide
Limitations: This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Publications:
  1. Bedard, J. E. J., Purnell, J. D., Ware, S. M. (2007) Nuclear import export signals are essential for proper cellular trafficking function of ZIC3, 16, 187-198. 2. Wang, J., Shao, Y., Bennett, T. A., Shankar, R. A., Wightman, P. D., Reddy, L. G. (2006) The Functional Effects of Physical Interactions among Toll-like Receptors 7, 8, 9, 281, 37427-37434.

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