Novus Biologicals, LLC
PO Box 802; Littleton, CO, 80160 USA
Phone: 303.730.1950 Fax: 303.730.1966
HA Epitope Tag Antibody (NB600-361)
HA Epitope Tag Antibody
| Catalog#: | NB600-361 |
This product is fully covered under guarantee+ |
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| Storage: | Store at 4C. Do not freeze. |
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| Buffer: | Phosphate Buffered Saline, pH 7.2 |
| Preservative: | 0.09% Sodium Azide |
| Purity: | Immunogen affinity purified |
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| Host: | Ch |
| Clonality: | Polyclonal |
| Specificity: | Antibody recognizes the HA cleavage site. |
| Modification: | No |
| Immunogen: | Chickens were immunized with HA cleavage site (YPYDVPDYA) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support. Chicken anti- human HA affinity purified antibodies were coupled to peptide immobilized on solid support. |
Background:
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA
molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
| NA | |
| reactivity: | Epitope Tag. Non-species specific.. |
| ICC, WB, ELISA, IF, IP | |
| Uses: | Suggested working dilutions: * ELISA Coating 1:100-1:500Primary 1:1000-1:30,000 Western Blots Colorimetric detection 1:1000-1:10,000ECL 1:1000-1:30,000 Cytochemistry 1:100-1:400 *The investigator should determine the optimal working dilution for a specific application. ELISA, Western blot, and immunocytochemistry. |
| Dilutions: | ELISA, Immunocytochemistry, immunofluorescence, immunoprecipitation, Western Blot |
| Publications: | Bedard, J. E. J., Purnell, J. D., Ware, S. M. (2007) Nuclear import export signals are essential for proper cellular trafficking function of ZIC3, 16, 187-198. 2. Wang, J., Shao, Y., Bennett, T. A., Shankar, R. A., Wightman, P. D., Reddy, L. G. (2006) The Functional Effects of Physical Interactions among Toll-like Receptors 7, 8, 9, 281, 37427-37434. |
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HA Epitope Tag in Context
Working with HA Epitope Tag
Publications (1)
Product Specific:
Bedard, J. E. J., Purnell, J. D., Ware, S. M. (2007) Nuclear import export signals are essential for proper cellular trafficking function of ZIC3, 16, 187-198.
2. Wang, J., Shao, Y., Bennett, T. A., Shankar, R. A., Wightman, P. D., Reddy, L. G. (2006) The Functional Effects of Physical Interactions among Toll-like Receptors 7, 8, 9, 281, 37427-37434.