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Clonality:	Monoclonal
Specificity:	The ACR 2 reacts with various forms of porcine acrosin (55, 53, 45 and 35 kDa) in the acrosome of undamaged spermatozoa.
Purity:	Affinity purified
Host:	Mouse

Immunogen:	Acid extracts of boar spermatozoa were subjected to hydrophobic chromatography and the pooled fraction with reactivity to N-alpha benzoylarginine-4-nitroanilide was used for immunization.
Clone:	ACR-2
Isotype:	IgG1

Acrosin is the major proteinase present in the acrosome of mature spermatozoa. It is a typical serine proteinase with trypsin-like specificity. It is stored in the acrosome in its precursor form, proacrosin. The active enzyme functions in the lysis of the zona pellucida, thus facilitating penetration of the sperm through the innermost glycoprotein layers of the ovum. The mRNA for proacrosin is synthesized only in the postmeiotic stages of spermatogenesis. In humans proacrosin first appears in the haploid spermatids.

	Porcine
Reactivity:	Cross-reacts with Pig.Does not cross-react with Cow, Dog or Human.Not yet tested in other species..
*Note not all species have been tested for reactivity. Only those species listed have been tested. We cannot make any guarantees about additional reactivities or cross reactivities beyond those that have been tested. This antibody may or may not react with other species.

	ICC, WB
Uses:	Immunocytochemistry, Western Blot ICC: Use at an assay dependent dilution membrane permeabilization (acetone) essential. The antibody ACR-2 reacts with various forms of porcine acrosin (55, 53, 45 and 35 kDa) in the acrosome of undamaged spermatozoa. Optimal dilutions and concentrations should be determined by the end user.
Dilutions:	Western Blot , Immunocytochemistry

Storage:	Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer:	PBS. pH 7.4
Preservative:	15mM Sodium Azide
Concentration:	1.0 mg/ml
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

1. This product has been used in:Moos J et al. Protein-protein interactions controlling acrosin release solubilization during the boar sperm acrosome reaction. Biol Reprod 49:408-15 (1993).
2. Peknicova J Moos J Monoclonal antibodies against boar acrosomal antigens labelling undamaged acrosomes of spermatozoa in immunofluorescence test. rologia 22:427-35 (1990).

2 publications available on website

ALB, TNF, CRP, SPINK6, CRIP2, DMBT1, CSRP1, GRPCB, IL6, ESR1, ES5R, RAB27A, AR, AKR1B1, INS, CTLA4, AKR1B7, AKR1B8, AREG, FDXR, AKR1B3, TNP2, HLA-DQA1, NOD2, LRP6, IL2, RAPGEF5, SLC17A5, ACRBP, ZP2, RANGAP1, IL10, CAT, TRPV6, PRM1, GLYAT, CRAT, PRM2, MIP, IL1RN