Novus Biologicals, LLC
PO Box 802; Littleton, CO, 80160 USA
Phone: 303.730.1950 Fax: 303.730.1966
Acrosin Antibody (NB500-419)
Acrosin Antibody (ACR-2)
| Catalog#: | NB500-419 |
This product is fully covered under guarantee+ |
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| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
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| Buffer: | PBS. pH 7.4 |
| Preservative: | 15mM Sodium Azide |
| Purity: | Affinity purified |
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| Host: | Mu |
| Clonality: | Monoclonal |
| Specificity: | The ACR 2 reacts with various forms of porcine acrosin (55, 53, 45 and 35 kDa) in the acrosome of undamaged spermatozoa. |
| Modification: | No |
| Clone: | ACR-2 |
| Isotype: | IgG1 |
| Immunogen: | Acid extracts of boar spermatozoa were subjected to hydrophobic chromatography and the pooled fraction with reactivity to N-alpha benzoylarginine-4-nitroanilide was used for immunization. |
| Related Genes: | 49 |
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Background:
Acrosin is the major proteinase present in the acrosome of mature spermatozoa. It is a typical serine proteinase with trypsin-like specificity. It is stored in the acrosome in its precursor form, proacrosin. The active enzyme functions in the lysis of the zona pellucida, thus facilitating penetration of the sperm through the innermost glycoprotein layers of the ovum. The mRNA for proacrosin is synthesized only in the postmeiotic stages of spermatogenesis. In humans proacrosin first appears in the haploid spermatids.
| Po | |
| reactivity: | Cross-reacts with Pig.Does not cross-react with Cow, Dog or Human.Not yet tested in other species.. |
| ICC, WB | |
| Uses: | Immunocytochemistry, Western Blot ICC: Use at an assay dependent dilution membrane permeabilization (acetone) essential. The antibody ACR-2 reacts with various forms of porcine acrosin (55, 53, 45 and 35 kDa) in the acrosome of undamaged spermatozoa. Optimal dilutions and concentrations should be determined by the end user. |
| Dilutions: | Western Blot , Immunocytochemistry |
| Publications: | This product has been used in:Moos J et al. Protein-protein interactions controlling acrosin release solubilization during the boar sperm acrosome reaction. Biol Reprod 49:408-15 (1993). Peknicova J Moos J Monoclonal antibodies against boar acrosomal antigens labelling undamaged acrosomes of spermatozoa in immunofluorescence test. rologia 22:427-35 (1990). |
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Acrosin in Context
Working with Acrosin
Publications (2)
General:
This product has been used in:Moos J et al. Protein-protein interactions controlling acrosin release solubilization during the boar sperm acrosome reaction. Biol Reprod 49:408-15 (1993).
Peknicova J Moos J Monoclonal antibodies against boar acrosomal antigens labelling undamaged acrosomes of spermatozoa in immunofluorescence test. rologia 22:427-35 (1990).