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Clonality:	Polyclonal
Specificity:	The specificity of this is for ABCG1.
Purity:	Peptide affinity purified
Host:	Rabbit

Immunogen:	A synthetic peptide made to an internal region of human ABCG1 (between residues 300-400).

Entrez:	9619 (Human)
	11307 (Mouse)
Swiss Prot:	P45844 (Human)

ATP-binding cassette (ABC) transporter genes are involved in the regulation of the amount of dietary cholesterol retained in the body. The ABC transporter ABCG1 (ABC8) is expressed on the cell surface and in intracellular compartments of cholesterol-laden macrophages and is induced in monocyte-derived macrophages during cholesterol influx mediated by acetylated low-density lipoprotein. In contrast, lipid efflux in cholesterol-laden macrophages, mediated by the cholesterol acceptor HDL(3), suppresses the expression of ABCG1.

	Mouse, Human
Reactivity:	This antibody reacts with mouse and human protein. Other species have not been tested. There is 100%/100% identity/homology of the immunogen used for this production and the human protein sequence. There is 84%/89% for mouse and 78%/88% for rat..
*Note not all species have been tested for reactivity. Only those species listed have been tested. We cannot make any guarantees about additional reactivities or cross reactivities beyond those that have been tested. This antibody may or may not react with other species.

	IHC-P, WB
Uses:	This antibody is useful for Western blot on mouse peritoneal macrophages treated with 22-R-hydroxycholesterol and T0901317 (stimulating ABCG1 expression) and immunohistochemistry. The theoretical molecular weight of ABCG1 is ~75.6 kDa, as seen by Western blot. Preliminary results suggest that this antibody may be also used for immunofluorescence on mouse ABCG1 overexpressed in HEK93 cells. It is not recommended for immunoprecipitation.
Dilutions:	Immunohistochemistry-Paraffin, Western Blot 1:500
Conjugated Ab's:	6 conjugated antibodies

Storage:	Store at 4C. Do not freeze.
Preservative:	0.1% Sodium Azide
Unit Size:	0.1ml (also available: 0.025ml (sample size))
Concentration:	1.0 mg/ml
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

1. Hu, W., S. Abe-Dohmae, et al. (2008). Biogenesis of HDL by SAA is dependent on ABCA1 in the liver in vivo. J. Lipid Res. 49: 386-393.
2. Tansley, Gavin H., et al. The Cholesterol Transporter ABCG1 Modulates The Subcellular Distribution Protecolytic Processing of B-Amyloid Precursor Protein. Journal of Lipid Research. doi: 10.1194/jlr.M600542-JLR200, 2007.
3. Wang, M., et al. Different cellular traffic of LDL-cholesterol acetylated LDL-cholesterol leads to distinct reverse cholesterol transport pathways. Journal of Lipid Research. 48: 633-645, 2007.

29 publications available on website

 

Western Blot: ABCG1 antibody [NB400-132] - Detection of ABCG1 in mouse peritoneal macrophages (40 ug), using NB 400-132. Lane 1: positive ABCG1 control, lane 2: 10 uM 22-(R)-hydrocholesterol treated macrophages, Lane 3: 100 nM T0901713 (LXRa ligand) treat

Immunohistochemistry: ABCG1 antibody [NB400-132] - Staining of lung, alveolar macrophages using NB400-132.

4 Peptides and Proteins

3 Primary Antibodies

7 RNAi

123 Secondary Antibodies

ABCA1, APOA1, NR1H3, SCARB1, TCF3, RFC1, UGT1A6, BRP1, ATP6V0A1, RFC4, KLRA1, RFC2, BCL2A1A, ABCA13, SREBF1, ABCG5, APOE, CD36, ABCG4, CETP, ABCG2, LDLR, ABCG8, CYP27A1, ABCA4, SREBF2, LCAT, ABCB1B, ABCB1, PPARG, TNF, LPL, FASN, TOM1L2, NR0B2, APP, PLTP, NPC1, TG, ABCB1A

General Protocols

• Protocol specific for ABCG1 Antibody (NB400-132)