Novus Biologicals, LLC
PO Box 802; Littleton, CO, 80160 USA
Phone: 303.730.1950 Fax: 303.730.1966


ABCG1 Antibody (NB400-132)

ABCG1 Antibody

Catalog#: NB400-132 100% Guarantee
This product is fully covered under guarantee+
Storage: Store at 4C. Do not freeze.
Preservative: 0.1% Sodium Azide
Purity: peptide affinity purified
Host: Rb
Clonality: Polyclonal
Specificity: The specificity of this is for ABCG1.
Modification: No
Immunogen: A synthetic peptide made to an internal region of human ABCG1 (between residues 300-400).
Gene Symbol: ABCG1 
Entrez: 9619 (Human)
11307 (Mouse)
Related Genes: 9619
Swiss Prot: P45844 (Human)
Background:
ATP-binding cassette (ABC) transporter genes are involved in the regulation of the amount of dietary cholesterol retained in the body. The ABC transporter ABCG1 (ABC8) is expressed on the cell surface and in intracellular compartments of cholesterol-laden macrophages and is induced in monocyte-derived macrophages during cholesterol influx mediated by acetylated low-density lipoprotein. In contrast, lipid efflux in cholesterol-laden macrophages, mediated by the cholesterol acceptor HDL(3), suppresses the expression of ABCG1.
Species:
Mu, Hu
reactivity: This antibody reacts with mouse and human protein. Other species have not been tested. There is 100%/100% identity/homology of the immunogen used for this production and the human protein sequence. There is 84%/89% for mouse and 78%/88% for rat..
Applications:
IHC-P, WB
Uses: This antibody is useful for Western blot on mouse peritoneal macrophages treated with 22-R-hydroxycholesterol and T0901317 (stimulating ABCG1 expression) and immunohistochemistry. The theoretical molecular weight of ABCG1 is ~75.6 kDa, as seen by Western blot. Preliminary results suggest that this antibody may be also used for immunofluorescence on mouse ABCG1 overexpressed in HEK93 cells. It is not recommended for immunoprecipitation.
Dilutions: Western Blot , Immunohistochemistry-Paraffin
Publications: Hu, W., S. Abe-Dohmae, et al. (2008). Biogenesis of HDL by SAA is dependent on ABCA1 in the liver in vivo. J. Lipid Res. 49: 386-393. Tansley, Gavin H., et al. The Cholesterol Transporter ABCG1 Modulates The Subcellular Distribution Protecolytic Processing of B-Amyloid Precursor Protein. Journal of Lipid Research. doi: 10.1194/jlr.M600542-JLR200, 2007. Wang, M., et al. Different cellular traffic of LDL-cholesterol acetylated LDL-cholesterol leads to distinct reverse cholesterol transport pathways. Journal of Lipid Research. 48: 633-645, 2007.
Protocols

General Protocols

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ABCG1 in Context

Research Areas related to ABCG1 (5):

Working with ABCG1

Publications (28)
Product Specific:

Hu, W., S. Abe-Dohmae, et al. (2008). Biogenesis of HDL by SAA is dependent on ABCA1 in the liver in vivo. J. Lipid Res. 49: 386-393.

Tansley, Gavin H., et al. The Cholesterol Transporter ABCG1 Modulates The Subcellular Distribution Protecolytic Processing of B-Amyloid Precursor Protein. Journal of Lipid Research. doi: 10.1194/jlr.M600542-JLR200, 2007.

Wang, M., et al. Different cellular traffic of LDL-cholesterol acetylated LDL-cholesterol leads to distinct reverse cholesterol transport pathways. Journal of Lipid Research. 48: 633-645, 2007.

28 product specific publications available on website.


 
Images (2)
Western Blot: ABCG1 antibody [NB400-132] - Detection of ABCG1 in mouse peritoneal macrophages (40 ug), using NB 400-132. Lane 1: positive ABCG1 control, lane 2: 10 uM 22-(R)-hydrocholesterol treated macrophages, Lane 3: 100 nM T0901713 (LXRa ligand) treat Western Blot: ABCG1 antibody [NB400-132] - Detection of ABCG1 in mouse peritoneal macrophages (40 ug), using NB 400-132. Lane 1: positive ABCG1 control, lane 2: 10 uM 22-(R)-hydrocholesterol treated macrophages, Lane 3: 100 nM T0901713 (LXRa ligand) treat Immunohistochemistry: ABCG1 antibody [NB400-132] - Staining of lung, alveolar macrophages using NB400-132. Immunohistochemistry: ABCG1 antibody [NB400-132] - Staining of lung, alveolar macrophages using NB400-132.

Protocols/Procedures (1)

Additional General Protocols

  • Protocol specific for ABCG1 Antibody (NB400-132)