ABCG1 Antibody

Species: Mu, Hu Applications: IHC-P, WB Clonality: Polyclonal

Gene: ABCG1 Purity: peptide affinity purified Host: Rabbit

Specificity:	The specificity of this is for ABCG1.
Publications:	Antibody has been mentioned in at least 28 publications.

Immunogen:	A synthetic peptide made to an internal region of human ABCG1 (between residues 300-400).

Entrez:	9619 (Human)
	11307 (Mouse)
Swiss Prot:	P45844 (Human)
Related Genes:	ABCA1, LXR, APOA1, PPARG, SCARB1, RFC2, RFC1, ATP6V0A1, RFC4, ABCG5, ABCG4, APOE, ABCG2, SREBF1, CD36, NR1H3, ABCG8, CYP27A1, LDLR, ABCA4, CETP, FASN, PPARA, LCAT, NR0B2, ABCB1, LPL, NPC1, ABCB4, SREBF2, CYP7A1, TG, PLTP, SREBP-2, CNBP, ABCA7, NR1H4, FAS, CCL2, CE

ATP-binding cassette (ABC) transporter genes are involved in the regulation of the amount of dietary cholesterol retained in the body. The ABC transporter ABCG1 (ABC8) is expressed on the cell surface and in intracellular compartments of cholesterol-laden macrophages and is induced in monocyte-derived macrophages during cholesterol influx mediated by acetylated low-density lipoprotein. In contrast, lipid efflux in cholesterol-laden macrophages, mediated by the cholesterol acceptor HDL(3), suppresses the expression of ABCG1.

Reactivity:	This antibody reacts with mouse and human protein. Other species have not been tested. There is 100%/100% identity/homology of the immunogen used for this production and the human protein sequence. There is 84%/89% for mouse and 78%/88% for rat.

Uses:	This antibody is useful for Western blot on mouse peritoneal macrophages treated with 22-R-hydroxycholesterol and T0901317 (stimulating ABCG1 expression) and immunohistochemistry. The theoretical molecular weight of ABCG1 is ~75.6 kDa, as seen by Western blot. Preliminary results suggest that this antibody may be also used for immunofluorescence on mouse ABCG1 overexpressed in HEK93 cells. It is not recommended for immunoprecipitation.
Dilutions:	Western Blot , Immunohistochemistry-Paraffin
Unit Size:	0.1ml (also available: 0.025ml (sample size))
Concentrations:	1.0 mg/ml
Conjugated Ab's:	6 conjugated antibodies

Storage:	Store at 4C. Do not freeze.
Preservative:	0.1% Sodium Azide
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.