ABCA1 Antibody [DyLight 549]

Species: Rt, Mu, Hu, Ha Applications: IHC-P, ICC, WB, IP, IF, FACS Clonality: Polyclonal

Gene: ABCA1 Purity: Affinity purified Host: Rabbit

Specificity:	This is specific for ABCA1.
Publications:	Antibody has been mentioned in at least 92 publications.

Conjugate:	DyLight 549
Immunogen:	Partial peptide sequence (peptide used resides somewhere between a.a 1100-1300) of the human ABCA1 gene. Actual immunogen sequence is proprietary information.
Localization:	Astrocyte Marker

Entrez:	19 (Human)
	313210 (Rat)
Swiss Prot:	O95477 (Human)
Related Genes:	APOA1, NR1H2, APOA1, APOA1, SCARB1, NR1H3, SCARB1, ATP6V0A1, KLRA1, RFC4, BRP1, RFC2, RFC1, TCF3, RFC4, BCL2A1A, ABCG1, ABCG1, ABCG1, PPARG, ABCA4, SCARB1, NR1H3, CD36, CD36, CD36, APOE, APOE, APOE, PPARA, PPARA, PPARG, ABCA13, ABCC3, LDLR, CXCL16, LDLR, TNF, NR1H3, SREBF1

Mutations in the ABCA1 gene (ATP-binding cassette transporter 1) have been reported in Tangier disease (TD). TD is an autosomal recessive disorder characterized by an absence of plasma HDL, cholesterol ester depositing in the reticulo-endothelial system and deviations in cellular lipid trafficking. ABCA1 mediates the apo-A1 associated export of cholesterol and phospholipids from the cell. It is expressed on the plasma membrane and the Golgi complex, and is regulated by cholesterol flux. Regulation of the cholesterol flux between HDL and macrophages is competitive between ABCA1 and SR-BI.

Reactivity:	NB 400-105 reacts with human, rat, hamster and mouse ABCA1. Other species have not been tested.

Uses:	NB 400-105 may be used for Western analysis where a band representing ABCA1 is seen at ~220 kDa. Additional non-specific bands are seen at lower molecular weights, but do not interfere with the ABCA1 signal. Other applications have been reported in literature using this antibody including immunofluorescence, immunohistochemistry, flow cytometry, and immunocytochemistry. NOTE: It is important not to boil the sample before loading onto a gel. Boiling can cause aggregation in large proteins, resulting in the proteins inability to enter the gel.
Dilutions:	flow cytometry / FACS analysis , immunofluorescence , immunoprecipitation , Western Blot 1:500, Immunocytochemistry , Immunohistochemistry-Paraffin 1:200
Concentrations:	1.0 mg/ml
Conjugated Ab's:	4 conjugated antibodies

Buffer:	50mM sodium borate
Storage:	Store at 4C. Do not freeze.
Preservative:	No Preservative
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.