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Clonality:	Polyclonal
Specificity:	This is specific for ABCA1.
Purity:	Affinity purified
Host:	Rabbit

Immunogen:	Partial peptide sequence (peptide used resides somewhere between a.a 1100-1300) of the human ABCA1 gene. Actual immunogen sequence is proprietary information.
Localization:	Astrocyte Marker

Entrez:	19 (Human)
	11303 (Mouse)
	313210 (Rat)
Swiss Prot:	O95477 (Human)

Mutations in the ABCA1 gene (ATP-binding cassette transporter 1) have been reported in Tangier disease (TD). TD is an autosomal recessive disorder characterized by an absence of plasma HDL, cholesterol ester depositing in the reticulo-endothelial system and deviations in cellular lipid trafficking. ABCA1 mediates the apo-A1 associated export of cholesterol and phospholipids from the cell. It is expressed on the plasma membrane and the Golgi complex, and is regulated by cholesterol flux. Regulation of the cholesterol flux between HDL and macrophages is competitive between ABCA1 and SR-BI.

	Rat, Mouse, Human
Reactivity:	NB 400-105 reacts with human, rat and mouse ABCA1. Other species have not been tested..
*Note not all species have been tested for reactivity. Only those species listed have been tested. We cannot make any guarantees about additional reactivities or cross reactivities beyond those that have been tested. This antibody may or may not react with other species.

	IHC-P, ICC, WB, IP, IF, FACS
Uses:	NB 400-105 may be used for Western analysis where a band representing ABCA1 is seen at ~220 kDa. Additional non-specific bands are seen at lower molecular weights, but do not interfere with the ABCA1 signal. Other applications have been reported in literature using this antibody including immunofluorescence, immunohistochemistry, flow cytometry, and immunocytochemistry. NOTE: It is important not to boil the sample before loading onto a gel. Boiling can cause aggregation in large proteins, resulting in the proteins inability to enter the gel.
Dilutions:	flow cytometry / FACS analysis , immunofluorescence , immunoprecipitation , Western Blot 1:500, Immunocytochemistry , Immunohistochemistry-Paraffin 1:200
Conjugated Ab's:	5 conjugated antibodies
Positive Controls:	Raw 264.7 Whole Cell [T0901317 treated and untreated lysates] Lysate (Raw 264.7 Whole Cell Lysate (NBP1-30159)(2 vials))

Storage:	Store at 4C. Do not freeze.
Buffer:	Tris-citrate pH 7-8
Preservative:	0.02% Sodium Azide
Unit Size:	0.1ml (also available: 0.025ml (sample size))
Concentration:	1.0 mg/ml
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

1. Crisafulli C, Di Paola R, Mazzon E, et al. Liver X receptor agonist treatment reduced splanchnic ischemia and reperfusion injury. J Leukoc Biol. 2009 Dec 22. [PMID: 20028773]
2. Murthy, S., et al. LXR/RXR activation enhances basolateral efflux of cholesterol in CaCo-2 cells. J. Lipid Research. 43: 1054-1064, 2002. [WB, human]
3. Burgess, J.W., et al. Trypsin-sensitive lipid-containing sites of the macrophage extracellular matrix bind apolipoprotein A-I participate in the ABCA-1-dependent cholesterol efflux. JBC Papers in Press. 277(35): 31318-31326, 2002. [WB, human/mouse]

103 publications available on website

Application: Western Blot on 06/15/2009

Images (3)

	Western Blot: ABCA1 Antibody [NB400-105] - Western Blot analysis of ABCA1, using NB400-105. Samples: 40 ug of total cell post-nuclear lysate of raw macrophages treated with 9-cisretinoic acid and 22R-hydroxycholesterol.		Western Blot: ABCA1 antibody [NB400-105] - Detection of ABCA1 in mouse peritoneal macrophages using NB 400-105 (Lot L). ECL exposure, 1 min. Lane 4: T09 uninduced lysate Lane 5: T09 induced lysate
	Immunohistochemistry: ABCA1 antibody [NB400-105] - Detection of ABCA1 in prostate epithelium showing luminal and membrane staining using NB400-105.

2 Peptides and Proteins

11 Primary Antibodies

1 RNAi

123 Secondary Antibodies

APOA1, NR1H2, NR1H3, SCARB1, ABCG1, RFC1, ATP6V0A1, BCL2A1A, RFC4, TCF3, RFC2, UGT1A6, BRP1, KLRA1, ABCA4, APOE, CD36, LDLR, CETP, ABCA13, SREBF1, TNF, LCAT, APP, LPL, INS, ABCG5, PPARA, ABCG8, SREBF2, PLTP, ARG1, H2-AB1, ABCA2, TG, CD320, NR1H4, PPARG, HMGCR, CYP27A1

General Protocols

• Protocol specific for ABCA1 Antibody (NB400-105)