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Clonality:	Monoclonal
Specificity:	This does not detect butyrylcholinesterase (BChE).
Purity:	Ascites
Host:	Mouse

Immunogen:	Purified human cerebellar acetylcholinesterase
Clone:	HR2
Isotype:	IgG2b
Localization:	Cell Membrane and Secreted

Entrez:	43 (Human)

Cholinergic neurotransmission occurs in motor, autonomic and central nervous synapses and requires very rapid inactivation of its transmitter, acetylcholine (ACh). Acetylcholinesterase (AChE) rapidly hydrolyzes ACh to acetate and choline, thereby inactivating it. AChE is found in the neuromuscular junction anchored to the basal lamina which runs between the nerve terminal and muscle membrane. AChE is also found outside the nervous and neuromuscular system in blood, lymph, germ and liver cells suggesting a role for AChE not related to cholinergic transmission. Another less specific cholinesterase, butyrylcholinesterase (BChE), seems to contribute to the regulation of the ACh concentration in the synaptic cleft.

	Feline, Rabbit, Human, Bovine
Reactivity:	Cross-reacts with Human, Rabbit, Cow, Cat and Guinea pig. Not yet tested in other species..
*Note not all species have been tested for reactivity. Only those species listed have been tested. We cannot make any guarantees about additional reactivities or cross reactivities beyond those that have been tested. This antibody may or may not react with other species.

	IHC-Fr, IP, IHC, ELISA
Uses:	Immunohistochemistry (frozen), ELISA, Immunoprecipitation. Immunohistochemical staining of AChE in human brain samples results in staining of nerve fibers and terminals. This antibody cannot be used in Western blot to detect AChE. This antibody is not suitable for FACS.
Dilutions:	ELISA assay dependent, immunohistochemistry 1:50, immunoprecipitation Assay dependent, Immunohistochemistry-Frozen 1:50
Positive Controls:	Acetylcholinesterase Lysate (Acetylcholinesterase Lysate (NBL1-07238)(0.1mg))

Storage:	Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer:	PBS
Preservative:	0.05% Sodium Azide
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

1. This product has been used in:Rakonczay Z Brimijoin S Monoclonal antibodies to human brain acetylcholinesterase: properties applications. Cell Mol Neurobiol 8:85-93 (1988).
2. Mesulam MM et al. Immunocytochemical demonstration of axonal perikaryal acetylcholinesterase in human cerebral cortex. Brain Res 539:233-8 (1991). PUBMED:2054599 J. Neural. Transm. 34:139-1450 (1991).

2 publications available on website

BCHE, CHAT, CAT, SH2D3C, CRAT, MIP, TRPV6, GLYAT, NGF, SPP1, TH, TAC1, APP, QPCT, G4, CHRNA2, VIP, NPY, NT5E, AFP, CSF2, H2-AB1, LAMC2, PNPLA6, GLUL, ACE, GSTA4, SST, SOD1, DLD, SLCO6A1, GSTK1, MGST1, DBH, CA1, ERMAP, CPAMD8, CAR1, ALB, GSR