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PO Box 802; Littleton, CO, 80160 USA
Phone: 303.730.1950 Fax: 303.730.1966
Acetylcholinesterase Antibody (NB300-528)
Acetylcholinesterase Antibody (HR2)
| Catalog#: | NB300-528 |
This product is fully covered under guarantee+ |
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| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
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| Buffer: | PBS |
| Preservative: | 0.05% Sodium Azide |
| Purity: | Ascites |
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| Host: | Mu |
| Clonality: | Monoclonal |
| Specificity: | This does not detect butyrylcholinesterase (BChE). |
| Modification: | No |
| Clone: | HR2 |
| Isotype: | IgG2b |
| Immunogen: | Purified human cerebellar acetylcholinesterase |
| Entrez: | 43 (Human) |
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| Related Genes: | 43 |
Background:
Cholinergic neurotransmission occurs in motor, autonomic and central nervous synapses and requires very rapid inactivation of its transmitter, acetylcholine (ACh). Acetylcholinesterase (AChE) rapidly hydrolyzes ACh to acetate and choline, thereby inactivating it. AChE is found in the neuromuscular junction anchored to the basal lamina which runs between the nerve terminal and muscle membrane. AChE is also found outside the nervous and neuromuscular system in blood, lymph, germ and liver cells suggesting a role for AChE not related to cholinergic transmission. Another less specific cholinesterase, butyrylcholinesterase (BChE), seems to contribute to the regulation of the ACh concentration in the synaptic cleft.
| Fe, Rb, Hu, Bv | |
| reactivity: | Cross-reacts with Human, Rabbit, Cow, Cat and Guinea pig. Not yet tested in other species.. |
| IHC-Fr, IP, IHC, ELISA | |
| Uses: | Immunohistochemistry (frozen), ELISA, Immunoprecipitation. Immunohistochemical staining of AChE in human brain samples results in staining of nerve fibers and terminals. This antibody cannot be used in Western blot to detect AChE. This antibody is not suitable for FACS. |
| Dilutions: | ELISA assay dependent, immunohistochemistry 1:50, immunoprecipitation Assay dependent, Immunohistochemistry-Frozen 1:50 |
| Positive Controls: | Acetylcholinesterase Lysate (Acetylcholinesterase Lysate (NBL1-07238)(0.1mg)) |
| Publications: | This product has been used in:Rakonczay Z Brimijoin S Monoclonal antibodies to human brain acetylcholinesterase: properties applications. Cell Mol Neurobiol 8:85-93 (1988). Mesulam MM et al. Immunocytochemical demonstration of axonal perikaryal acetylcholinesterase in human cerebral cortex. Brain Res 539:233-8 (1991). PUBMED:2054599 J. Neural. Transm. 34:139-1450 (1991). |
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Working with Acetylcholinesterase
Publications (2)
General:
This product has been used in:Rakonczay Z Brimijoin S Monoclonal antibodies to human brain acetylcholinesterase: properties applications. Cell Mol Neurobiol 8:85-93 (1988).
Mesulam MM et al. Immunocytochemical demonstration of axonal perikaryal acetylcholinesterase in human cerebral cortex. Brain Res 539:233-8 (1991). PUBMED:2054599 J. Neural. Transm. 34:139-1450 (1991).