ACCN4 Antibody

Catalog#:	NB110-74734	This product is fully covered under guarantee+

Storage:	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer:	Lyophilized
Preservative:	No Preservative

Purity:	Whole antisera
Host:	Rb
Clonality:	Polyclonal
Specificity:	ASIC4.
Modification:	No
Immunogen:	A synthetic peptide as a part of human, rat and mouse amiloride-sensitive cation channel 4 (ACCN4, ASIC4) conjugated to immunogenic carrier protein was used as the immunogen.

Entrez:	55515 (Human)
	241118 (Mouse)
	63882 (Rat)
Omim:	ASIC4_HUMAN ; ASIC4_RAT ; ASIC4_MOUSE (Human)
Related Genes:	55515
Swiss Prot:	Q96FT7 (Human)

ACCN4 belongs to the superfamily of acid-sensing ion channels, which are proton-gated, amiloride-sensitive sodium channels. These channels have been implicated in synaptic transmission, pain perception as well as mechanoperception. ACCN4 is predominantly expressed in the pituitary gland, and might be a candidate for paroxysmal dystonic choreoathetosis (PDC), a movement disorder. FUNCTION: Probable cation channel with high affinity for sodium. These channels have been implicated in synaptic transmission, pain perception as well as mechanoperception. SUBUNIT: Homotetramer or heterotetramer with other ASIC proteins (Probable). SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: This gene is predominantly expressed in the pituitary gland. Expressed in brain, spinal chord and dorsal root ganglion (DRG). Expressed by a subset of sensory neurons in the DRG. Expressed by granule cells in the cerebellar cortex. In hippocampus, expression is detected in dentate gyrus granule cells, in pyramidal cells of CA1-CA3 subfields and in interneurons of the striatum oriens and radiatum of all subfields. In cerebral cortex expressed in small, medium and large pyramidal cells in layers 2, 3 and 5 respectively. Also expressed in striatum, globus pallidus, inferior and superior calliculi, amygdala, magnocellular preoptic nucleus, islands of Calleja and large neurons of olfactory tubercules. DEVELOPMENTAL STAGE: Highly expressed in newborn spinal chord but hardly detected in the cerebellum compared to adult. Expressed at postnatal day 1 in ependymal cells lining the central canal of spinal chord and in motor neurons. In adult, expression decreases in ependymal cells and increases in motor neurons. The number of positive interneurons decreases but the individual interneuron expression increases in adult spinal chord compared to newborn. MISCELLANEOUS: In vitro, has no proton-gated channel activity.

	Mu, Rt, Hu
reactivity:	Human, Rat and Mouse. .

	WB, IHC
Uses:	Immunohistochemistry and Western Blot
Dilutions:	immunohistochemistry Western Blot 1:300
Positive Controls:	ACCN4 Lysate (ACCN4 Lysate (NBL1-07233)(0.1mg))

Publications:	Gruender S, et al. NeuroReport 11:1607-1611(2000). Akopian A.N, et al. NeuroReport 11:2217-2222(2000). 2 publications available on website

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Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. When reconstituting, glycerol (1:1) may be added for an additional stability.