Novus Reward Points: Review this product for a reward and claim prizes (novusbio.com/about/rewards) 100 points for each review and 100 for an image We value both positive and negative feedback! Reviews can be done at www.novusbio.com/reviews/1/NB100-79865/988818/1166149

We stand behind our products 100%. If you cannot get a product to work in an application or species stated on our datasheet, our technical service team will troubleshoot with you to get it to work. If a product still does not work after troubleshooting, you can receive a free of charge replacement product or a full refund. Our Novus Guarantee + covers 100% of the products we carry. No hassles, no nonsense. It is that simple! If you decide to use a product in an application or species for which it has not been tested, and you report to us your success or failure, we will give you a significant discount on the future purchase of another product. This discount can be up to 80% of your purchase price.

Clonality:	Polyclonal
Specificity:	ACVR1
Purity:	Immunogen affinity purified
Host:	Goat

Immunogen:	Synthetic peptide, C-RKFKRRNQERLNPRD, representing the internal region of the sequence according to NP_001096.1
Epitope:	RKFKRRNQERLNPRD
Isotype:	IgG

Entrez:	90 (Human)
	11477 (Mouse)
	79558 (Rat)
Swiss Prot:	NP_001096.1 (Human)

Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I ( I and IB) and two type II (II and IIB) receptors. These receptors are all transmembrane proteins, composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling; and type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. This gene encodes activin A type I receptor which signals a particular transcriptional response in concert with activin type II receptors. Mutations in this gene are associated with fibrodysplasia ossificans progressive.

	Rat, Mouse, Human, Canine
Reactivity:	Human, mouse, rat and dog. .
*Note not all species have been tested for reactivity. Only those species listed have been tested. We cannot make any guarantees about additional reactivities or cross reactivities beyond those that have been tested. This antibody may or may not react with other species.

	ELISA, WB, IHC
Uses:	This antibody is useful for Peptide ELISA, Immunohistochemistry and Western Blot. Approx 60kDa band observed in Human Umbilical Cord lysates (calculated MW of 57.2kDa according to NP_001096.1). Immunohistochemistry: In paraffin embedded Human Testis shows preferential staining of cytoplasm in primary spermatocytes.
Dilutions:	ELISA 1:128000, immunohistochemistry 4-6µg/ml, Western Blot 0.3-1µg/ml

Storage:	Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer:	Tris-saline [pH7.3] with 0.5% BSA
Preservative:	0.02% Sodium Azide
Concentration:	0.5 mg/ml
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

1. Shore EM, Xu M, Feldman GJ, Fenstermacher DA, Cho TJ, Choi IH, Connor JM, Delai P, Glaser DL, LeMerrer M, Morhart R, Rogers JG, Smith R, Triffitt JT, Urtizberea JA, Zasloff M, Brown MA, Kaplan FS. A recurrent mutation in the BMP type I receptor ACVR1 causes inherited sporadic fibrodysplasia ossificans progressiva. Nat Genet. 2006 May;38(5):525-7. Epub 2006 Apr 23. Erratum in: Nat Genet. 2007 Feb;39(2):276. FOP International Research Consortium

 

Western Blot: ACVR1 Antibody [NB100-79865] - NB100-79865 (0.3ug/ml) staining of Human Umbilical Cord lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry: ACVR1 Antibody [NB100-79865]- (4µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

3 Peptides and Proteins

9 Primary Antibodies

1 RNAi

95 Secondary Antibodies

BMP4, BMP7, BMPR1A, BMP2, SMAD1, ACVR1B, GARS, ACVRL1, BMPR1B, ACVR2A, ACVR2B, TGFBR1, INHBA, BMPR2, SLPI, SMAD2, BMP6, FST, SMAD5, TGFB1, KCNA1, ALK, TDGF1, PPM1A, FGFR1, SMAD4, NOG, NODAL, TGFBR3, SMAD9, TNF, ACVR1C, BRD2, ENG, VEGFA, GDF2, EGF, ATP8A2, ZMYM2, SMAD3