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Activin A Receptor Type I Antibody (NB100-79865)

Activin A Receptor Type I Antibody

Catalog#:	NB100-79865	This product is fully covered under guarantee+

Storage:	Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer:	Tris-saline [pH7.3] with 0.5% BSA
Preservative:	0.02% Sodium Azide

Purity:	Immunogen affinity purified
Host:	Gt
Clonality:	Polyclonal
Specificity:	ACVR1
Modification:	No
Isotype:	IgG
Immunogen:	Synthetic peptide, C-RKFKRRNQERLNPRD, representing the internal region of the sequence according to NP_001096.1
Epitope:	RKFKRRNQERLNPRD

Gene Symbol: ACVR1

Entrez:	90 (Human)
	11477 (Mouse)
	79558 (Rat)
Related Genes:	90
Swiss Prot:	NP_001096.1 (Human)

Background:

Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I ( I and IB) and two type II (II and IIB) receptors. These receptors are all transmembrane proteins, composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling; and type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. This gene encodes activin A type I receptor which signals a particular transcriptional response in concert with activin type II receptors. Mutations in this gene are associated with fibrodysplasia ossificans progressive.

Species:

	Rt, Mu, Hu, Ca
reactivity:	Human, mouse, rat and dog. .

Applications:

	ELISA, WB, IHC
Uses:	This antibody is useful for Peptide ELISA, Immunohistochemistry and Western Blot. Approx 60kDa band observed in Human Umbilical Cord lysates (calculated MW of 57.2kDa according to NP_001096.1). Immunohistochemistry: In paraffin embedded Human Testis shows preferential staining of cytoplasm in primary spermatocytes.
Dilutions:	ELISA 1:128000, immunohistochemistry 4-6µg/ml, Western Blot 0.3-1µg/ml

Publications:	Shore EM, Xu M, Feldman GJ, Fenstermacher DA, Cho TJ, Choi IH, Connor JM, Delai P, Glaser DL, LeMerrer M, Morhart R, Rogers JG, Smith R, Triffitt JT, Urtizberea JA, Zasloff M, Brown MA, Kaplan FS. A recurrent mutation in the BMP type I receptor ACVR1 causes inherited sporadic fibrodysplasia ossificans progressiva. Nat Genet. 2006 May;38(5):525-7. Epub 2006 Apr 23. Erratum in: Nat Genet. 2007 Feb;39(2):276. FOP International Research Consortium

Publications:

Shore EM, Xu M, Feldman GJ, Fenstermacher DA, Cho TJ, Choi IH, Connor JM, Delai P, Glaser DL, LeMerrer M, Morhart R, Rogers JG, Smith R, Triffitt JT, Urtizberea JA, Zasloff M, Brown MA, Kaplan FS. A recurrent mutation in the BMP type I receptor ACVR1 causes inherited sporadic fibrodysplasia ossificans progressiva. Nat Genet. 2006 May;38(5):525-7. Epub 2006 Apr 23. Erratum in: Nat Genet. 2007 Feb;39(2):276. FOP International Research Consortium

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Activin A Receptor Type I in Context

Research Areas related to Activin A Receptor Type I (1):

Protein Kinase

Working with Activin A Receptor Type I

Publications (1)

General:

Images (2)

Western Blot: ACVR1 Antibody [NB100-79865] - NB100-79865 (0.3ug/ml) staining of Human Umbilical Cord lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry: ACVR1 Antibody [NB100-79865]- (4µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.