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Clonality:	Monoclonal
Specificity:	This antibody has shown cross-reactivity with actin 1, 2, 3, 4, 7, 8, 11 and 12.
Purity:	Ascites
Host:	Mouse

Immunogen:	Purified Arabidopsis actin protein.
Clone:	mAbGEa
Isotype:	IgG1

Entrez:	58 (Human)
	11459 (Mouse)
	29437 (Rat)
	60 (ACTB)
	71 (ACTG1)

Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in nonmuscle cells.

	Plant, Bovine, Rat, Mouse, Sheep, Human, Zebra Fish, Drosophila, Xenopus, Yeast
Reactivity:	Yeast, Xenopus laevis, Drosophila, Zebra Fish, Human, Sheep, Mouse, Rat, Bovine and Arabidopsis..
*Note not all species have been tested for reactivity. Only those species listed have been tested. We cannot make any guarantees about additional reactivities or cross reactivities beyond those that have been tested. This antibody may or may not react with other species.

	WB, ELISA
Uses:	ELISA and Western Blot In WB detects a 45 kDa protein representing actin.
Dilutions:	ELISA , Western Blot 1:1000

Storage:	Store at -20C. Avoid freeze-thaw cycles.
Preservative:	0.05% Sodium Azide
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

1. Shulga N, Hoek JB, Pastorino JG. J Biol Chem. Elevated PTEN levels account for the increased sensitivity of ethanol-exposed cells to tumor necrosis factor-induced cytotoxicity. 2005 Mar 11;280(10):9416-24. [pubmed: 15623531]
2. Trost SU, Belke DD, Bluhm WF, Meyer M, et al. Overexpression of the sarcoplasmic reticulum Ca(2+)-ATPase improves myocardial contractility in diabetic cardiomyopathy. Diabetes. 2002 Apr;51(4):1166-71. [pubmed: 11916940]

2 publications available on website

Application: Western Blot on 11/18/2009

Images (2)

Western Blot: Actin Antibody (mAbGEa) [NB100-74340] - MDCK cell extract

Western Blot: pan Actin Antibody (mAbGEa) [NB100-74340] - illustrates Western blot detection of actin in MDCK cell extract using NB100-74340

7 Primary Antibodies

216 Secondary Antibodies

QPCT, ALB, PLB1, CALM3, CALM2, CALM1, CD74, ST6GALNAC2, VIPR1, TAF8, GCNT2, VIM, DES, PLA2G1B, INS, YWHAZ, CYCS, ESR1, SLC11A2, CAT, ZBTB8OS, TRF, TNIP1, NEB, MYH14, BBS9, PREP, PRB2, ES5R, TF, APOA1, AP3B1, CD34, ACHE, SERPINA1, TPM3, HNRNPC, GSTP1, GRXCR1, TYR