Novus Biologicals, LLC
PO Box 802; Littleton, CO, 80160 USA
Phone: 303.730.1950 Fax: 303.730.1966
pan Actin Antibody (NB100-74340)
pan Actin Antibody (mAbGEa)
| Catalog#: | NB100-74340 |
This product is fully covered under guarantee+ |
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| Storage: | Store at -20C. Avoid freeze-thaw cycles. |
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| Preservative: | 0.05% Sodium Azide |
| Purity: | Ascites |
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| Host: | Mu |
| Clonality: | Monoclonal |
| Specificity: | Actin (mAbGEa) |
| Modification: | No |
| Clone: | mAbGEa |
| Isotype: | IgG1 |
| Immunogen: | Purified Arabidopsis actin protein. |
| Entrez: | 58 (Human) |
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| 11459 (Mouse) | |
| 29437 (Rat) | |
| 60 (ACTB) | |
| 71 (ACTG1) | |
| Related Genes: | 58 |
Background:
Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in nonmuscle cells.
| Pl, Bv, Rt, Mu, Sh, Hu, Ze, Dr, Xp, Ye | |
| reactivity: | Yeast, Xenopus laevis, Drosophila, Zebra Fish, Human, Sheep, Mouse, Rat, Bovine and Arabidopsis.. |
| WB, ELISA | |
| Uses: | ELISA and Western Blot In WB detects a 45 kDa protein representing actin. |
| Dilutions: | ELISA , Western Blot 1:1000 |
| Publications: | Shulga N, Hoek JB, Pastorino JG. J Biol Chem. Elevated PTEN levels account for the increased sensitivity of ethanol-exposed cells to tumor necrosis factor-induced cytotoxicity. 2005 Mar 11;280(10):9416-24. [pubmed: 15623531] Trost SU, Belke DD, Bluhm WF, Meyer M, et al. Overexpression of the sarcoplasmic reticulum Ca(2+)-ATPase improves myocardial contractility in diabetic cardiomyopathy. Diabetes. 2002 Apr;51(4):1166-71. [pubmed: 11916940] |
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Reviews
There are 1 reviews for this product.
Working with pan Actin
Publications (2)
General:
Shulga N, Hoek JB, Pastorino JG. J Biol Chem. Elevated PTEN levels account for the increased sensitivity of ethanol-exposed cells to tumor necrosis factor-induced cytotoxicity. 2005 Mar 11;280(10):9416-24. [pubmed: 15623531]
Trost SU, Belke DD, Bluhm WF, Meyer M, et al. Overexpression of the sarcoplasmic reticulum Ca(2+)-ATPase improves myocardial contractility in diabetic cardiomyopathy. Diabetes. 2002 Apr;51(4):1166-71. [pubmed: 11916940]
Images (1)
![Western Blot: Actin Antibody (mAbGEa) [NB100-74340] - MDCK cell extract](/sites/default/files/100-74340.jpg "Western Blot: Actin Antibody (mAbGEa) [NB100-74340] - MDCK cell extract")
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Western Blot: Actin Antibody (mAbGEa) [NB100-74340] - MDCK cell extract |
Reviews (1) Have you used this product? Submit a review to earn Novus Rewards.
Carol Bookwalter on 31/12/69
| Primary Antibody: | Diluted |
|---|---|
| Dilution | 1/1000 |
| Incubation Dilution Buffer: | |
| Incubation Time: | Overnight |
| Incubation Temp: | 4 |
| Amount Loaded: | Control Lane - #7 is 1ug |
| Gel Details: | Lane1 perfect proteins Lane2 total lysate Lane3 Clarified lysate Lane4 After overnight dialysis Lane5 Claified overnight dialysis Lane6 Column flow through Lane7 Purified yeast actin (S. cerevisiae) |
| Detection Method | DAB |
| Used Controls: | True |
| Observed Bands: | True |
| Specific Bands: | ~45kD |
| Nonspecific Bands: | No |
| Secondary Name: | Goat anti mouse HRP |
|---|---|
| Host Species: | |
| Conjugation: | HRP |
| Diluted? | Diluted |
| Dilution: | 1/2000 |
| Blocking Buffer: | powdered milk in PBS |
| Blocking Concentration: | 3% |
| Blocking Time: | 30 minutes |
| Blocking Temp | Room temp |