14-3-3 Antibody

Species: Hu Applications: WB Clonality: Polyclonal

Gene: YWHAQ Purity: protein G purified Host: Sheep

Specificity:

NB100-62100 recognizes the lower molecular weight subunit (26 kD) of protein 14-3-3. The 14-3-3 family consists of 30kD proteins that are involved in multiple cellular processes including protein kinase signalling pathways, regulation of cell cycle progression, transcription and intracellular trafficking. Protein interactions with 14-3-3 show distinct preferences for its different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein.

Isotype:	IgG
Immunogen:	Native, purified from pathogen-free normal human brain.
Localization:	Cytoplasmic

Entrez:	10971 (Human)
Related Genes:	MAPT, MAPT, MAPT, TH, CSNK1E, AKT1, PRNP, AKT1, LAMC2, AKT1, RAF1, MAPK1, MAPK1, CDC25C, CDC25C, PIK3CG, BCL2L1, BCL2L1, BCL2L1, RAF1, CASP3, MAPK8, MAPK1, PRNP, TNF, RAF1, PTK2B, CHEK1, CHEK1, GSTP1, CHEK1, GSTA4, ZHX2, PRKACA, RASGRF1, MAPK8, ZHX2, ZHX2, HCLS1, GSK3B

Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf 1 kinase activity and phosphorylation amoung many other functions.

Reactivity:	Human. Not yet tested in other species.

Uses:	This antibody is useful for Western Blot
Dilutions:	Western Blot 1:10 - 1:50
Concentrations:	2.5 mg/ml
Positive Controls:	1 positive controls

Buffer:	Phosphate buffered saline, pH 7.2
Storage:	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Preservative:	0.09% Sodium Azide
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.