Novus Biologicals, LLC
PO Box 802; Littleton, CO, 80160 USA
Phone: 303.730.1950 Fax: 303.730.1966


ACOX2 Antibody (NB100-57853)

ACOX2 Antibody

Catalog#: NB100-57853 100% Guarantee
This product is fully covered under guarantee+
Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer: Tris-saline [ pH7.3] with 0.5% BSA
Preservative: 0.02% Sodium Azide
Purity: Immunogen affinity purified
Host: Gt
Clonality: Polyclonal
Specificity: ACOX2
Modification: No
Isotype: IgG
Immunogen: Synthetic peptide, C-QWAQKSPTNTQENP, representing the internal region (near the C Terminus) of the human sequence according to NP_003491.1
Epitope: QWAQKSPTNTQENP
Gene Symbol: ACOX2 
Entrez: 8309 (Human)
Related Genes: 8309
Swiss Prot: NP_003491.1 (Human)
Background:
The product of this gene belongs to the acyl-CoA oxidases family. It encodes the branched-chain acyl-CoA oxidase which is involved in the degradation of long branched fatty acids and bile acid intermediates in peroxisomes. Deficiency of this enzyme results in the accumulation of branched fatty acids and bile acid intermediates, and may lead to Zellweger syndrome, severe mental retardation and death in children.
Species:
Rb, Hu
reactivity: Human and Rabbit. .
Applications:
WB, ELISA
Uses: This antibody is useful for Peptide ELISA and Western Blot. Approx 75kDa band observed in Human Liver lysates (calculated MW of 76.8kDa according to NP_003491.1). An additional band of 37kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.
Dilutions: ELISA 1:4000, Western Blot 0.5-1.5µg/ml
Positive Controls: Whole Normal Liver Lysate (Whole Normal Liver Lysate (NB820-59232)(1mg))
Publications: Baumgart E, Vanhooren JC, Fransen M, Marynen P, Puype M, Vekerckhove J, Leunissen JA, Fahimi HD, Mannaerts GP, van Veldhoven PP. Molecular characterization of the human peroxisomal branched-chain acyl-CoA oxidase: cDNA cloning, chromosomal assignment, tissue distribution, evidence for the absence of the protein in Zellweger syndrome.Proc Natl Acad Sci U S A. 1996 Nov 26;93(24):13748-53. pubmed: 8943006
Reviews
No reviews have been submitted for this product.

Working with ACOX2

Publications (1)
General:

Baumgart E, Vanhooren JC, Fransen M, Marynen P, Puype M, Vekerckhove J, Leunissen JA, Fahimi HD, Mannaerts GP, van Veldhoven PP. Molecular characterization of the human peroxisomal branched-chain acyl-CoA oxidase: cDNA cloning, chromosomal assignment, tissue distribution, evidence for the absence of the protein in Zellweger syndrome.Proc Natl Acad Sci U S A. 1996 Nov 26;93(24):13748-53. pubmed: 8943006


 
Images (1)
Western Blot: ACOX2 Antibody [NB100-57853] - NB100-57853 (0.5µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence. Western Blot: ACOX2 Antibody [NB100-57853] - NB100-57853 (0.5µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.