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Clonality:	Polyclonal
Specificity:	ACYP1
Purity:	Immunogen affinity purified
Host:	Goat

Immunogen:	Synthetic peptide, C-PKSHIDKANFNNEK, representing the internal region of the human protein according to NP_001098.1
Epitope:	PKSHIDKANFNNEK
Isotype:	IgG

Entrez:	97 (Human)
Swiss Prot:	NP_001098.1 (Human)

Acylphosphatase is a small cytosolic enzyme that catalyzes the hydrolysis of the carboxyl-phosphate bond of acylphosphates. Two isoenzymes have been isolated, called muscle acylphosphatase and erythrocyte acylphosphatase, on the basis of their tissue localization. This gene encodes the erythrocyte acylphosphatase isoenzyme. Alternatively spliced transcript variants that encode different proteins were identified through data analysis.

	Rat, Mouse, Human
Reactivity:	Human, mouse and rat. .
*Note not all species have been tested for reactivity. Only those species listed have been tested. We cannot make any guarantees about additional reactivities or cross reactivities beyond those that have been tested. This antibody may or may not react with other species.

	WB, ELISA
Uses:	This antibody is useful for Peptide ELISA and Western Blot. Approx 15kDa band observed in Human Testis lysates (calculated MW of 11.3kDa according to NP_001098.1).
Dilutions:	ELISA 1:128000, Western Blot 0.01-0.03µg/ml
Positive Controls:	Whole Normal Testis Lysate (Whole Normal Testis Lysate (NB820-59266)(1.0mg)) ACYP1 Lysate (ACYP1 Lysate (NBL1-07302)(0.1mg))

Storage:	Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer:	Tris-saline [ pH7.3] with 0.5% BSA
Preservative:	0.02% Sodium Azide
Concentration:	0.5 mg/ml
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

1. Paoli P, Pazzagli L, Giannoni E, Caselli A, Manao G, Camici G, Ramponi G. A nucleophilic catalysis step is involved in the hydrolysis of aryl phosphate monoesters by human CT acylphosphatase. J Biol Chem. 2003 Jan 3;278(1):194-9. Epub 2002 Oct 29. pubmed: 12409302

 

Western Blot: ACYP1 Antibody [NB100-57091] - NB100-57091 (0.01ug/ml) staining of Human Testes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

1 Peptides and Proteins

2 Primary Antibodies

2 RNAi

95 Secondary Antibodies

FADD, FJX1, CD44, JDP2, APIP, CD109, ZNF410, JAK2, TRIM44, PDHX, ABCD4, SLC1A1, CTTN, TRAF6, CD274, LTBP2, COMMD9, PPFIA1, ISCA2, ZFP410, RLN1, COQ6, LTBP3, HBLD1, MYO6