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PO Box 802; Littleton, CO, 80160 USA
Phone: 303.730.1950 Fax: 303.730.1966


ACYP1 Antibody (NB100-57091)

ACYP1 Antibody

Catalog#: NB100-57091 100% Guarantee
This product is fully covered under guarantee+
Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer: Tris-saline [ pH7.3] with 0.5% BSA
Preservative: 0.02% Sodium Azide
Purity: Immunogen affinity purified
Host: Gt
Clonality: Polyclonal
Specificity: ACYP1
Modification: No
Isotype: IgG
Immunogen: Synthetic peptide, C-PKSHIDKANFNNEK, representing the internal region of the human protein according to NP_001098.1
Epitope: PKSHIDKANFNNEK
Gene Symbol: ACYP1 
Entrez: 97 (Human)
Related Genes: 97
Swiss Prot: NP_001098.1 (Human)
Background:
Acylphosphatase is a small cytosolic enzyme that catalyzes the hydrolysis of the carboxyl-phosphate bond of acylphosphates. Two isoenzymes have been isolated, called muscle acylphosphatase and erythrocyte acylphosphatase, on the basis of their tissue localization. This gene encodes the erythrocyte acylphosphatase isoenzyme. Alternatively spliced transcript variants that encode different proteins were identified through data analysis.
Species:
Rt, Mu, Hu
reactivity: Human, mouse and rat. .
Applications:
WB, ELISA
Uses: This antibody is useful for Peptide ELISA and Western Blot. Approx 15kDa band observed in Human Testis lysates (calculated MW of 11.3kDa according to NP_001098.1).
Dilutions: ELISA 1:128000, Western Blot 0.01-0.03µg/ml
Positive Controls: Whole Normal Testis Lysate (Whole Normal Testis Lysate (NB820-59266)(1mg)) ACYP1 Lysate (ACYP1 Lysate (NBL1-07302)(0.1mg))
Publications: Paoli P, Pazzagli L, Giannoni E, Caselli A, Manao G, Camici G, Ramponi G. A nucleophilic catalysis step is involved in the hydrolysis of aryl phosphate monoesters by human CT acylphosphatase. J Biol Chem. 2003 Jan 3;278(1):194-9. Epub 2002 Oct 29. pubmed: 12409302
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Working with ACYP1

Publications (1)
General:

Paoli P, Pazzagli L, Giannoni E, Caselli A, Manao G, Camici G, Ramponi G. A nucleophilic catalysis step is involved in the hydrolysis of aryl phosphate monoesters by human CT acylphosphatase. J Biol Chem. 2003 Jan 3;278(1):194-9. Epub 2002 Oct 29. pubmed: 12409302


 
Images (1)
Western Blot: ACYP1 Antibody [NB100-57091] - NB100-57091 (0.01ug/ml) staining of Human Testes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Western Blot: ACYP1 Antibody [NB100-57091] - NB100-57091 (0.01ug/ml) staining of Human Testes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.