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TRAF3IP2 Antibody (NB100-56317)

TRAF3IP2 Antibody

Catalog#:	NB100-56317	This product is fully covered under guarantee+

Storage:	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer:	PBS
Preservative:	0.02% Sodium Azide

Purity:	protein G purified
Host:	Rb
Clonality:	Polyclonal
Specificity:	In MCF-7, an approx. 60 kDa band is observed.
Modification:	No
Isotype:	IgG
Immunogen:	A mixture of synthetic peptides corresponding to amino acids 341-351 (CSFPGLPRHQDQPHHQ), 91-104 (CLRTQVLEDSEDSF) and 562-574 (PRGPLPTLQVVPLC) of human ACT1 was used as immunogen, GenBank no. gi\|10242290\|gb\|AAG15367.1\|AF274303_1 .

Gene Symbol: TRAF3IP2

Entrez:	10758 (Human)
Related Genes:	10758

Background:

NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus (3). External stimuli such as tumor necrosis factor or other cytokines, such as IL-1 results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes (2). Recently, Li et al. (1) have isolated a cDNA coding for a protein named Act1 (NF- B activator 1). Act1 cDNA codes for a protein containing 574 aa with a predicated molecular mass of 60 kDa. Act1 protein contains a helix-loop-helix domain in its N-terminal portion and a coiled-coil structure at the C terminus. Act1 lacks any catalytic domain, however, it activates IKK through the helix-loop-helix domain. The activation of NF- B by Act1 appears to be through the NIK-IKK complex, because a NIK dominant-negative mutant protein inhibited Act1-induced NF- B activation. IL-1 responsive pathway is also necessary for Act1 function. Act1 mRNA is expressed in a variety of tissues, with high level in thymus, kidney and placenta; moderate level in heart, skeletal muscle, colon, liver, lung and small intestine; and at a very low level in brain, spleen, and peripheral blood leukocytes.

Species:

	Hu
reactivity:	Cross reacts with Human. .

Applications:

	WB
Uses:	Western Blot
Dilutions:	Western Blot 1-2 ug/ml
Positive Controls:	MCF-7 Whole Cell Lysate (MCF-7 Whole Cell Lysate (NB820-59465)(1mg))	TRAF3IP2 Lysate (TRAF3IP2 Lysate (NBL1-17243)(0.1mg))

Publications:	Verma, I.M., Stevenson, J.K., Schwarz, EM., Van Antwerp, D. Miyamoto, S. Genes. Dev. 9: 2723-2735 (1995). Verma, I. Stevenson, J.K. Proc. Nat. Acad. Sci. USA 94: 11758 (1997). Li X, Commane M, Nie H, Hua X, Chatterjee-Kishore M, Wald D, Haag M, Stark GR. Proc Natl Acad Sci U S A 97(19):10489-10493 (2000). 3 publications available on website

Reviews

No reviews have been submitted for this product.

TRAF3IP2 in Context

Research Areas related to TRAF3IP2 (1):

Signal Transduction

Working with TRAF3IP2

Publications (3)

General:

Verma, I.M., Stevenson, J.K., Schwarz, EM., Van Antwerp, D. Miyamoto, S. Genes. Dev. 9: 2723-2735 (1995).

Verma, I. Stevenson, J.K. Proc. Nat. Acad. Sci. USA 94: 11758 (1997).

Li X, Commane M, Nie H, Hua X, Chatterjee-Kishore M, Wald D, Haag M, Stark GR. Proc Natl Acad Sci U S A 97(19):10489-10493 (2000).

Images (1)

Western Blot: ACT1 Antibody [NB100-56317] - Western blot analysis of 10 ug of total cell lysate from MCF-7, a human breast cancer cell line, with NB100-56317 at 1 ug/ml dilution.