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Clonality:	Polyclonal
Specificity:	In MCF-7, an approx. 60 kDa band is observed.
Purity:	Protein G purified
Host:	Rabbit

Immunogen:	A mixture of synthetic peptides corresponding to amino acids 341-351 (CSFPGLPRHQDQPHHQ), 91-104 (CLRTQVLEDSEDSF) and 562-574 (PRGPLPTLQVVPLC) of human ACT1 was used as immunogen, GenBank no. gi|10242290|gb|AAG15367.1|AF274303_1 .
Isotype:	IgG

Entrez:	10758 (Human)

NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus (3). External stimuli such as tumor necrosis factor or other cytokines, such as IL-1 results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes (2). Recently, Li et al. (1) have isolated a cDNA coding for a protein named Act1 (NF- B activator 1). Act1 cDNA codes for a protein containing 574 aa with a predicated molecular mass of 60 kDa. Act1 protein contains a helix-loop-helix domain in its N-terminal portion and a coiled-coil structure at the C terminus. Act1 lacks any catalytic domain, however, it activates IKK through the helix-loop-helix domain. The activation of NF- B by Act1 appears to be through the NIK-IKK complex, because a NIK dominant-negative mutant protein inhibited Act1-induced NF- B activation. IL-1 responsive pathway is also necessary for Act1 function. Act1 mRNA is expressed in a variety of tissues, with high level in thymus, kidney and placenta; moderate level in heart, skeletal muscle, colon, liver, lung and small intestine; and at a very low level in brain, spleen, and peripheral blood leukocytes.

	Human
Reactivity:	Cross reacts with Human. .
*Note not all species have been tested for reactivity. Only those species listed have been tested. We cannot make any guarantees about additional reactivities or cross reactivities beyond those that have been tested. This antibody may or may not react with other species.

	WB
Uses:	Western Blot
Dilutions:	Western Blot 1-2 ug/ml
Positive Controls:	MCF-7 Whole Cell Lysate (MCF-7 Whole Cell Lysate (NB820-59465)(1mg)) TRAF3IP2 Lysate (TRAF3IP2 Lysate (NBL1-17243)(0.1mg))

Storage:	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer:	PBS
Preservative:	0.02% Sodium Azide
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

1. Verma, I.M., Stevenson, J.K., Schwarz, EM., Van Antwerp, D. Miyamoto, S. Genes. Dev. 9: 2723-2735 (1995).
2. Verma, I. Stevenson, J.K. Proc. Nat. Acad. Sci. USA 94: 11758 (1997).
3. Li X, Commane M, Nie H, Hua X, Chatterjee-Kishore M, Wald D, Haag M, Stark GR. Proc Natl Acad Sci U S A 97(19):10489-10493 (2000).

3 publications available on website

 

Western Blot: ACT1 Antibody [NB100-56317] - Western blot analysis of 10 ug of total cell lysate from MCF-7, a human breast cancer cell line, with NB100-56317 at 1 ug/ml dilution.

1 Lysates

2 Peptides and Proteins

12 Primary Antibodies

2 RNAi

123 Secondary Antibodies

NFKB1, TNF, IL17A, TRAF6, GUSB, ACT1, ACT2, CCL4, CD40, JUND, JUNB, FOSB, FOS, JUN, SLC25A4, GAL, LOC222344, NAT1, IKBKB, MAPK8, CD40LG, IL1B, LGALS1, IKBKE, IKBKG, CHUK, PLS1, ANGPTL4, TNFSF13B, MAP3K7, ACTR3B, RELA, PGK1, ACTL6A, CD8A, APOBEC3B, DEFB4A, PARK2, CXCL1, RACGAP1