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Clonality:	Polyclonal
Specificity:	This is specific for HIF-1 alpha.
Purity:	Immunogen affinity purified
Host:	Rabbit

Immunogen:	A fusion protein including residues 530-825 of the mouse HIF-1 alpha protein.

Entrez:	3091 (Human)
	15251 (Mouse)
	29560 (Rat)
	Q61221 (Mouse)

Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF hydroxylases provide insight into hypoxic cell responses, which may be used to help isolate therapeutic targets.

	Rat, Primate, Mouse, Human
Reactivity:	This antibody reacts against monkey, mouse, rat, and human protein..
*Note not all species have been tested for reactivity. Only those species listed have been tested. We cannot make any guarantees about additional reactivities or cross reactivities beyond those that have been tested. This antibody may or may not react with other species.

	IHC-Fr, IHC-P, WB
Uses:	This antibody has been tested in Western blot, recognizing a band in hypoxic samples at ~115 kDa. It has only been tested against mouse, rat, human and primate protein. Rat retina and brain cortex have been tested. Rat brain cortex is a bit dirty but this antibody does detect upregulation of HIF-1 alpha in hypoxic samples. This antibody is not recommended for immunoprecipitation. It has also been used for immunohistochemistry (see procedure guide).
Dilutions:	Immunohistochemistry-Frozen 5-10 ug/ml, Immunohistochemistry-Paraffin 5-10 ug/ml, Western Blot 1:500-1:2000
Conjugated Ab's:	5 conjugated antibodies
Positive Controls:	RCC4 Lysate (RCC4 Lysate (NBP1-30412)(1.0mg)) COS-7 [Hypoxia induced and uninduced Nuclear Extract Kit] Lysate (COS-7 Lysate (NB800-PC26)(4 vials))

Storage:	Store at 4C. Do not freeze.
Buffer:	Tris-glycine, 150mM NaCl
Preservative:	0.05% Sodium Azide
Unit Size:	0.1ml (also available: 0.025ml (sample size))
Concentration:	1.0 mg/ml
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

1. Ramanathan M, Pinhal-Enfield G, Hao I, et al. Synergistic up-regulation of vascular endothelial growth factor (VEGF) expression in macrophages by adenosine A2A receptor agonists and endotoxin involves transcriptional regulation via the hypoxia response element in the VEGF promoter. Mol Biol Cell. 2007 Jan;18(1):14-23. [PMID: 17065555] (Mouse, WB)
2. Bayele HK, Peyssonnaux C, Giatromanolaki A, et al. HIF-1 regulates heritable variation and allele expression phenotypes of the macrophage immune response gene SLC11A1 from a Z-DNA forming microsatellite. Blood. 2007 Oct 15;110(8):3039-48. [PMID: 1760676] (Mouse, WB)
3. Kundu CN, Balusu R, Jaiswal AS, et al. Adenomatous polyposis coli-mediated hypersensitivity of mouse embryonic fibroblast cell lines to methylmethane sulfonate treatment: implication of base excision repair pathways. Carcinogenesis. 2007 Oct;28(10):2089-95. [PMID: 1752206] (Mouse, WB)

16 publications available on website

 

Western Blot: HIF-1 alpha Antibody [NB100-479] - Detection of HIF-1 alpha in a hypoxic sample. Lane 1: CoCl2 treated Cos-7 nuclear extract (50 ug, hypoxic). Lane 2: Untreated Cos-7 nuclear extract (50 ug, normoxic). 10 second ECL exposure.

immunohistochemistry: HIF-1 alpha [NB100-479] - Staining of placenta, villi using NB100-479.

3 Antibody Packs

1 Antibody Pairs

1 Lysates

4 Peptides and Proteins

63 Primary Antibodies

2 RNAi

123 Secondary Antibodies

VEGFA, SETD2, ARNT, EPO, VHL, EPAS1, AKT1, MAPK3, SLC2A1, MIA3, TRP53, NOS2, CA9, TP53, PTGS2, MAPK1, KDR, TNF, EPX, EGLN1, JUN, MTOR, PIK3R1, EP300, PIK3CG, PIK3CB, NFKB1, NOTCH1, BCL2, SLC1A3, HMOX1, IL6, CDKN1A, CASP3, MMP2, HIF1AN, CAR9, EGFR, PSMC6, MMP9

General Protocols

• Protocol specific for HIF-1 alpha Antibody (NB100-479)

FAQs (1) (Available Online)

• How do you induce HIF-1 alpha in samples?