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Clonality:	Polyclonal
Specificity:	ACSL5
Purity:	Immunogen affinity purified
Host:	Goat

Immunogen:	C-RTQIDSLYEHIQDC TerminusNP_057318.2; NP_976313.1; NP_976314.1

Entrez:	51703 (Human)
Swiss Prot:	NP_057318.2; NP_976313.1; NP_976314.1 (Human)

The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in uterus and spleen, and in trace amounts in normal brain, but has markedly increased levels in malignant gliomas. This gene functions in mediating fatty acid-induced glioma cell growth. Three transcript variants encoding two different isoforms have been found for this gene.

	Human
Reactivity:	Human. .
*Note not all species have been tested for reactivity. Only those species listed have been tested. We cannot make any guarantees about additional reactivities or cross reactivities beyond those that have been tested. This antibody may or may not react with other species.

	WB, PEP-ELISA
Uses:	This antibody is useful for Peptide ELISA an Western Blot. Western Blot: Approx 75kDa band observed in human spleen lysates.
Dilutions:	Peptide ELISA , Western Blot

Storage:	Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer:	Tris-saline (pH7.3) with 0.5% BSA
Preservative:	0.02% Sodium Azide
Concentration:	0.5 mg/ml
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

1. Obermuller N, Keith M, Kopitz J, Autschbach F, Schirmacher P, Gassler N. Coeliac disease is associated with impaired expression of acyl-CoA-synthetase 5.Int J Colorectal Dis. 2005 Apr 5; [Epub ahead of print] pubmed: 15809837

 

Western Blot: ACSL5 Antibody [NB100-2918] - NB100-2918 (1ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

1 Antibody Pairs

2 Lysates

3 Peptides and Proteins

5 Primary Antibodies

3 RNAi

95 Secondary Antibodies

ACSL1, ACSS2, PLA2G15, ACCS, ACSL4, ACSL3, ACSL6, TWIST1, ACACA, PPARG, ACOX2, KLK15, PPARA, LYST, GUSB, CLU, ECHS1, EHHADH, GCDH, A2M, INS, PZP, ATF7IP, SGCG, ACADS, C9ORF7, FASN, CX3CL1, CD36, CAT, PCX, ACOT7, FABP4, SQLE, SLC27A1, MAPK1, HNRNPC, CYCS, GORAB, ACO1