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TOR/mTOR Antibody

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Product Details

Summary
Product Discontinued
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    • Catalog Number
      NB100-241
    • Availability
      Product Discontinued

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TOR/mTOR Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 200 and 250 of human Mammalian Target of Rapamycin using the numbering given in entry NP_004949.1 (GeneID 2475).
Specificity
This is specific for Mammalian Target of Rapamycin (mTOR).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MTOR
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunoprecipitation 2 - 10 ug/mg lysate
  • Western Blot 1:1000 - 1:5000
Application Notes
Western analysis: An overnight incubation at 4 degrees Celcius is recommended for primary antibody use. mTOR is ~235 kDa and optimally requires detergent and extended Western blotting transfer times. For example from 5% acrylamide gels to PVDF: 50V for 180 min. in CAPS buffer with 10% MeOH and 0.1% SDS was found to be optimal. Immunohistochemistry/Immunocytochemistry is not recommended. Please use NB100-240. Nuclear extracts are recommended.
Control
TOR/mTOR Overexpression Lysate
Publications
Read Publication using NB100-241.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TOR/mTOR Antibody

  • EC 2.7.11.1
  • FK506 binding protein 12-rapamycin associated protein 1
  • FK506 binding protein 12-rapamycin associated protein 2
  • FK506-binding protein 12-rapamycin complex-associated protein 1
  • FKBP12-rapamycin complex-associated protein
  • FLJ44809
  • FRAP
  • FRAP1
  • FRAP1FKBP12-rapamycin complex-associated protein 1
  • FRAP2
  • Mammalian target of rapamycin
  • mechanistic target of rapamycin (serine/threonine kinase)
  • Mechanistic target of rapamycin
  • MTOR
  • RAFT1
  • rapamycin and FKBP12 target 1
  • rapamycin associated protein FRAP2
  • Rapamycin target protein 1
  • RAPT1FKBP-rapamycin associated protein
  • serine/threonine-protein kinase mTOR
  • TOR

Background

FKBP 12 rapamycin associated protein (FRAP) is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, it is a 235 kDa protein (symbolized RAFT 1) with significant homology to the Saccharomyces cerevisiae protein TOR 1 and has been shown to associate with the immunophilin FKBP 12 in a rapamycin-dependent fashion. The FKBP 12 rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of FRAP to FKBP 12 rapamycin correlated with the ability of these ligands to inhibit cell cycle progression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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TOR/mTOR Antibody
NB100-241
TOR/mTOR Antibody
Species: Hu, Mu, Rt
Applications: WB, IP

Publications for TOR/mTOR Antibody (NB100-241)(1)


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Control Lysate(s)

TOR/mTOR Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

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Research Areas for TOR/mTOR Antibody (NB100-241)

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Blogs on TOR/mTOR.

Check out the latest blog posts on TOR/mTOR.
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mTOR Signaling and the Tumor Microenvironment
By Yoskaly Lazo-Fernandez, PhD The mammalian target of rapamycin (mTOR) is a conserved serine/threonine kinase that, as a member of two distinct intracellular protein complexes, mTORC1 and mTORC2, regulates protein ...  Read full blog post.
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mTOR - a central regulator of cell metabolism
The mammalian target of rapamycin (mTOR) signaling pathway allows cells to monitor environmental signals like nutrient availability and oxygen levels. mTOR is a phosphoinositide 3-kinase (PI3K)-related protein that assembles into large protein comp...  Read full blog post.

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Bioinformatics

Gene Symbol MTOR